Loss of tumor suppressor menin expression in high grade cholangiocarcinomas

BACKGROUND: MEN1, which codes for the protein menin, is a tumor suppressor in neuroendocrine tissue. In cholangiocarcinoma (CCA) cell lines the overexpression of menin decreased proliferation, angiogenesis, migration, and invasion in vitro and in xenografts, but its expression in CCA tumor tissue sa...

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Autores principales: Lairmore, Terry C., Abdulsattar, Jehan, De Benedetti, Arrigo, Shi, Runhua, Huang, Shile, Khalil, Md Imtiaz, Witt, Stephan N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9923918/
https://www.ncbi.nlm.nih.gov/pubmed/36782257
http://dx.doi.org/10.1186/s13104-023-06282-6
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author Lairmore, Terry C.
Abdulsattar, Jehan
De Benedetti, Arrigo
Shi, Runhua
Huang, Shile
Khalil, Md Imtiaz
Witt, Stephan N.
author_facet Lairmore, Terry C.
Abdulsattar, Jehan
De Benedetti, Arrigo
Shi, Runhua
Huang, Shile
Khalil, Md Imtiaz
Witt, Stephan N.
author_sort Lairmore, Terry C.
collection PubMed
description BACKGROUND: MEN1, which codes for the protein menin, is a tumor suppressor in neuroendocrine tissue. In cholangiocarcinoma (CCA) cell lines the overexpression of menin decreased proliferation, angiogenesis, migration, and invasion in vitro and in xenografts, but its expression in CCA tumor tissue samples is not established. OBJECTIVE: Determine whether the expression of menin correlates with disease progression in patient samples of CCA in a tissue microarray (TMA) by immunohistochemical (IHC) staining. RESULTS: IHC analysis of 97 biopsies revealed that low-grade tumors (Grade I) exhibited intense, diffuse, finely granular nuclear menin immunoreactivity with a pronounced linear perinuclear pattern (mean IHC score = 2.00), whereas high-grade tumors (Grade III) mostly lacked such staining (mean IHC score = 0.35). Collectively, there was a significant inverse association between tumor grade and menin staining (P = 0.0005). We also found a significant association between fibrosis status and menin staining, in that, 81.2% (56/69) of patients without fibrosis had no menin staining, whereas 92.9% (26/28) patients with fibrosis exhibited menin staining (P < 0.0001). No association was found between fibrosis status and grade. Overall, menin expression is inversely associated with tumor grade and positively associated with fibrosis status. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-023-06282-6.
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spelling pubmed-99239182023-02-14 Loss of tumor suppressor menin expression in high grade cholangiocarcinomas Lairmore, Terry C. Abdulsattar, Jehan De Benedetti, Arrigo Shi, Runhua Huang, Shile Khalil, Md Imtiaz Witt, Stephan N. BMC Res Notes Research Note BACKGROUND: MEN1, which codes for the protein menin, is a tumor suppressor in neuroendocrine tissue. In cholangiocarcinoma (CCA) cell lines the overexpression of menin decreased proliferation, angiogenesis, migration, and invasion in vitro and in xenografts, but its expression in CCA tumor tissue samples is not established. OBJECTIVE: Determine whether the expression of menin correlates with disease progression in patient samples of CCA in a tissue microarray (TMA) by immunohistochemical (IHC) staining. RESULTS: IHC analysis of 97 biopsies revealed that low-grade tumors (Grade I) exhibited intense, diffuse, finely granular nuclear menin immunoreactivity with a pronounced linear perinuclear pattern (mean IHC score = 2.00), whereas high-grade tumors (Grade III) mostly lacked such staining (mean IHC score = 0.35). Collectively, there was a significant inverse association between tumor grade and menin staining (P = 0.0005). We also found a significant association between fibrosis status and menin staining, in that, 81.2% (56/69) of patients without fibrosis had no menin staining, whereas 92.9% (26/28) patients with fibrosis exhibited menin staining (P < 0.0001). No association was found between fibrosis status and grade. Overall, menin expression is inversely associated with tumor grade and positively associated with fibrosis status. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-023-06282-6. BioMed Central 2023-02-13 /pmc/articles/PMC9923918/ /pubmed/36782257 http://dx.doi.org/10.1186/s13104-023-06282-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Lairmore, Terry C.
Abdulsattar, Jehan
De Benedetti, Arrigo
Shi, Runhua
Huang, Shile
Khalil, Md Imtiaz
Witt, Stephan N.
Loss of tumor suppressor menin expression in high grade cholangiocarcinomas
title Loss of tumor suppressor menin expression in high grade cholangiocarcinomas
title_full Loss of tumor suppressor menin expression in high grade cholangiocarcinomas
title_fullStr Loss of tumor suppressor menin expression in high grade cholangiocarcinomas
title_full_unstemmed Loss of tumor suppressor menin expression in high grade cholangiocarcinomas
title_short Loss of tumor suppressor menin expression in high grade cholangiocarcinomas
title_sort loss of tumor suppressor menin expression in high grade cholangiocarcinomas
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9923918/
https://www.ncbi.nlm.nih.gov/pubmed/36782257
http://dx.doi.org/10.1186/s13104-023-06282-6
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