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Fucosyltransferase 8 (FUT8) and core fucose expression in oxidative stress response

GlycoMaple is a new tool to predict glycan structures based on the expression levels of 950 genes encoding glycan biosynthesis-related enzymes and proteins using RNA-seq data. The antioxidant response, protecting cells from oxidative stress, has been focused on because its activation may relieve pat...

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Autores principales: Kyunai, Yuki M., Sakamoto, Mika, Koreishi, Mayuko, Tsujino, Yoshio, Satoh, Ayano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9924996/
https://www.ncbi.nlm.nih.gov/pubmed/36780470
http://dx.doi.org/10.1371/journal.pone.0281516
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author Kyunai, Yuki M.
Sakamoto, Mika
Koreishi, Mayuko
Tsujino, Yoshio
Satoh, Ayano
author_facet Kyunai, Yuki M.
Sakamoto, Mika
Koreishi, Mayuko
Tsujino, Yoshio
Satoh, Ayano
author_sort Kyunai, Yuki M.
collection PubMed
description GlycoMaple is a new tool to predict glycan structures based on the expression levels of 950 genes encoding glycan biosynthesis-related enzymes and proteins using RNA-seq data. The antioxidant response, protecting cells from oxidative stress, has been focused on because its activation may relieve pathological conditions, such as neurodegenerative diseases. Genes involved in the antioxidant response are defined within the GO:0006979 category, including 441 human genes. Fifteen genes overlap between the glycan biosynthesis-related genes defined by GlycoMaple and the antioxidant response genes defined by GO:0006979, one of which is FUT8. 5-Hydroxy-4-phenyl-butenolide (5H4PB) extracted from Chinese aromatic vinegar induces the expression of a series of antioxidant response genes that protect cells from oxidative stress via activation of the nuclear factor erythroid 2-related factor 2–antioxidant response element pathway. Here, we show that FUT8 is upregulated in both our RNA-seq data set of 5H4PB-treated cells and publicly available RNA-seq data set of cells treated with another antioxidant, sulforaphane. Applying our RNA-seq data set to GlycoMaple led to a prediction of an increase in the core fucose of N-glycan that was confirmed by flow cytometry using a fucose-binding lectin. These results suggest that FUT8 and core fucose expression may increase upon the antioxidant response.
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spelling pubmed-99249962023-02-14 Fucosyltransferase 8 (FUT8) and core fucose expression in oxidative stress response Kyunai, Yuki M. Sakamoto, Mika Koreishi, Mayuko Tsujino, Yoshio Satoh, Ayano PLoS One Research Article GlycoMaple is a new tool to predict glycan structures based on the expression levels of 950 genes encoding glycan biosynthesis-related enzymes and proteins using RNA-seq data. The antioxidant response, protecting cells from oxidative stress, has been focused on because its activation may relieve pathological conditions, such as neurodegenerative diseases. Genes involved in the antioxidant response are defined within the GO:0006979 category, including 441 human genes. Fifteen genes overlap between the glycan biosynthesis-related genes defined by GlycoMaple and the antioxidant response genes defined by GO:0006979, one of which is FUT8. 5-Hydroxy-4-phenyl-butenolide (5H4PB) extracted from Chinese aromatic vinegar induces the expression of a series of antioxidant response genes that protect cells from oxidative stress via activation of the nuclear factor erythroid 2-related factor 2–antioxidant response element pathway. Here, we show that FUT8 is upregulated in both our RNA-seq data set of 5H4PB-treated cells and publicly available RNA-seq data set of cells treated with another antioxidant, sulforaphane. Applying our RNA-seq data set to GlycoMaple led to a prediction of an increase in the core fucose of N-glycan that was confirmed by flow cytometry using a fucose-binding lectin. These results suggest that FUT8 and core fucose expression may increase upon the antioxidant response. Public Library of Science 2023-02-13 /pmc/articles/PMC9924996/ /pubmed/36780470 http://dx.doi.org/10.1371/journal.pone.0281516 Text en © 2023 Kyunai et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kyunai, Yuki M.
Sakamoto, Mika
Koreishi, Mayuko
Tsujino, Yoshio
Satoh, Ayano
Fucosyltransferase 8 (FUT8) and core fucose expression in oxidative stress response
title Fucosyltransferase 8 (FUT8) and core fucose expression in oxidative stress response
title_full Fucosyltransferase 8 (FUT8) and core fucose expression in oxidative stress response
title_fullStr Fucosyltransferase 8 (FUT8) and core fucose expression in oxidative stress response
title_full_unstemmed Fucosyltransferase 8 (FUT8) and core fucose expression in oxidative stress response
title_short Fucosyltransferase 8 (FUT8) and core fucose expression in oxidative stress response
title_sort fucosyltransferase 8 (fut8) and core fucose expression in oxidative stress response
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9924996/
https://www.ncbi.nlm.nih.gov/pubmed/36780470
http://dx.doi.org/10.1371/journal.pone.0281516
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