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T-type Ca(2+) channels and their relationship with pre-neoplastic and neoplastic lesions in the human breast

The expression of T-type voltage-dependent Ca(2+) channels (Cav3) has been previously observed in breast cancer, but their expression and subcellular localization were not evaluated in pre-neoplastic lesions. Therefore, this work aimed to evaluate protein expression and subcellular localization of T...

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Autores principales: Aguiar, F., Rhana, P., Bloise, E., Nunes, C.B., Rodrigues, A.L., Ferreira, E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Divulgação Científica 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9925191/
https://www.ncbi.nlm.nih.gov/pubmed/36790286
http://dx.doi.org/10.1590/1414-431X2023e11879
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author Aguiar, F.
Rhana, P.
Bloise, E.
Nunes, C.B.
Rodrigues, A.L.
Ferreira, E.
author_facet Aguiar, F.
Rhana, P.
Bloise, E.
Nunes, C.B.
Rodrigues, A.L.
Ferreira, E.
author_sort Aguiar, F.
collection PubMed
description The expression of T-type voltage-dependent Ca(2+) channels (Cav3) has been previously observed in breast cancer, but their expression and subcellular localization were not evaluated in pre-neoplastic lesions. Therefore, this work aimed to evaluate protein expression and subcellular localization of T-type channel isoforms in human breast tissue samples. Protein expressions of Ca(V)3.1, Ca(V)3.2, and Ca(V)3.3 were evaluated by immunohistochemistry in breast without alteration, in proliferative non-neoplastic lesions, and in neoplastic ductal epithelial lesions of the human breast. Ca(V)3.1, Ca(V)3.2, and Ca(V)3.3 nuclear expressions were decreased in advanced stages of neoplastic transformation, whereas Ca(V)3.1 and Ca(V)3.2 cytoplasmic expression increased. Also, the decrease in nuclear expression was correlated with an increase in cytoplasmic expression for Ca(V)3.1 isoform. The change in Ca(V)3 protein expression and subcellular localization are consistent with the neoplastic transformation stages of mammary epithelial cells, evident in early neoplastic lesions, such as ductal carcinomas in situ. These results suggest a possible involvement of Ca(V)3 in the carcinogenic processes and could be considered as a potential pharmacological target in new therapies for breast cancer treatment.
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spelling pubmed-99251912023-02-14 T-type Ca(2+) channels and their relationship with pre-neoplastic and neoplastic lesions in the human breast Aguiar, F. Rhana, P. Bloise, E. Nunes, C.B. Rodrigues, A.L. Ferreira, E. Braz J Med Biol Res Research Article The expression of T-type voltage-dependent Ca(2+) channels (Cav3) has been previously observed in breast cancer, but their expression and subcellular localization were not evaluated in pre-neoplastic lesions. Therefore, this work aimed to evaluate protein expression and subcellular localization of T-type channel isoforms in human breast tissue samples. Protein expressions of Ca(V)3.1, Ca(V)3.2, and Ca(V)3.3 were evaluated by immunohistochemistry in breast without alteration, in proliferative non-neoplastic lesions, and in neoplastic ductal epithelial lesions of the human breast. Ca(V)3.1, Ca(V)3.2, and Ca(V)3.3 nuclear expressions were decreased in advanced stages of neoplastic transformation, whereas Ca(V)3.1 and Ca(V)3.2 cytoplasmic expression increased. Also, the decrease in nuclear expression was correlated with an increase in cytoplasmic expression for Ca(V)3.1 isoform. The change in Ca(V)3 protein expression and subcellular localization are consistent with the neoplastic transformation stages of mammary epithelial cells, evident in early neoplastic lesions, such as ductal carcinomas in situ. These results suggest a possible involvement of Ca(V)3 in the carcinogenic processes and could be considered as a potential pharmacological target in new therapies for breast cancer treatment. Associação Brasileira de Divulgação Científica 2023-02-10 /pmc/articles/PMC9925191/ /pubmed/36790286 http://dx.doi.org/10.1590/1414-431X2023e11879 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Aguiar, F.
Rhana, P.
Bloise, E.
Nunes, C.B.
Rodrigues, A.L.
Ferreira, E.
T-type Ca(2+) channels and their relationship with pre-neoplastic and neoplastic lesions in the human breast
title T-type Ca(2+) channels and their relationship with pre-neoplastic and neoplastic lesions in the human breast
title_full T-type Ca(2+) channels and their relationship with pre-neoplastic and neoplastic lesions in the human breast
title_fullStr T-type Ca(2+) channels and their relationship with pre-neoplastic and neoplastic lesions in the human breast
title_full_unstemmed T-type Ca(2+) channels and their relationship with pre-neoplastic and neoplastic lesions in the human breast
title_short T-type Ca(2+) channels and their relationship with pre-neoplastic and neoplastic lesions in the human breast
title_sort t-type ca(2+) channels and their relationship with pre-neoplastic and neoplastic lesions in the human breast
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9925191/
https://www.ncbi.nlm.nih.gov/pubmed/36790286
http://dx.doi.org/10.1590/1414-431X2023e11879
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