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High-resolution synchrotron K-edge subtraction CT allows tracking and quantifying therapeutic cells and their scaffold in a rat model of focal cerebral injury and can serve as a reference for spectral photon counting CT

Background: The objective of this study was to demonstrate that synchrotron K-edge subtraction tomography (SKES-CT) can simultaneously track therapeutic cells and their encapsulating carrier, in vivo, in a rat model of focal brain injury using a dual-contrast agent approach. The second objective was...

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Autores principales: Tavakoli, Clément, Cuccione, Elisa, Dumot, Chloé, Balegamire, Joëlle, Si-Mohamed, Salim Aymeric, Kim, Johoon, Crola-da-Silva, Claire, Chevalier, Yves, Boussel, Loïc, Douek, Philippe, Cormode, David, Elleaume, Hélène, Brun, Emmanuel, Wiart, Marlène
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9925349/
https://www.ncbi.nlm.nih.gov/pubmed/36793350
http://dx.doi.org/10.7150/ntno.79575
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author Tavakoli, Clément
Cuccione, Elisa
Dumot, Chloé
Balegamire, Joëlle
Si-Mohamed, Salim Aymeric
Kim, Johoon
Crola-da-Silva, Claire
Chevalier, Yves
Boussel, Loïc
Douek, Philippe
Cormode, David
Elleaume, Hélène
Brun, Emmanuel
Wiart, Marlène
author_facet Tavakoli, Clément
Cuccione, Elisa
Dumot, Chloé
Balegamire, Joëlle
Si-Mohamed, Salim Aymeric
Kim, Johoon
Crola-da-Silva, Claire
Chevalier, Yves
Boussel, Loïc
Douek, Philippe
Cormode, David
Elleaume, Hélène
Brun, Emmanuel
Wiart, Marlène
author_sort Tavakoli, Clément
collection PubMed
description Background: The objective of this study was to demonstrate that synchrotron K-edge subtraction tomography (SKES-CT) can simultaneously track therapeutic cells and their encapsulating carrier, in vivo, in a rat model of focal brain injury using a dual-contrast agent approach. The second objective was to determine if SKES-CT could be used as a reference method for spectral photon counting tomography (SPCCT). Methods: Phantoms containing different concentrations of gold and iodine nanoparticles (AuNPS/INPs) were imaged with SKES-CT and SPCCT to assess their performances. A pre-clinical study was performed in rats with focal cerebral injury which intracerebrally received AuNPs-labelled therapeutic cells encapsulated in a INPs-labelled scaffold. Animals were imaged in vivo with SKES-CT and back-to-back with SPCCT. Results: SKES-CT revealed to be reliable for quantification of gold and iodine, whether alone or mixed. In the preclinical model, SKES-CT showed that AuNPs remained at the site of cell injection, while INPs expanded within and/or along the lesion border, suggesting dissociation of both components in the first days post-administration. Compared to SKES-CT, SPCCT was able to correctly locate gold, but not completely located iodine. When SKES-CT was used as reference, SPCCT gold quantification appeared very accurate both in vitro and in vivo. Iodine quantification by SPCCT was also quite accurate, albeit less so than for gold. Conclusion: We here provide the proof-of-concept that SKES-CT is a novel method of choice for performing dual-contrast agent imaging in the context of brain regenerative therapy. SKES-CT may also serve as ground truth for emerging technologies such as multicolour clinical SPCCT.
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spelling pubmed-99253492023-02-14 High-resolution synchrotron K-edge subtraction CT allows tracking and quantifying therapeutic cells and their scaffold in a rat model of focal cerebral injury and can serve as a reference for spectral photon counting CT Tavakoli, Clément Cuccione, Elisa Dumot, Chloé Balegamire, Joëlle Si-Mohamed, Salim Aymeric Kim, Johoon Crola-da-Silva, Claire Chevalier, Yves Boussel, Loïc Douek, Philippe Cormode, David Elleaume, Hélène Brun, Emmanuel Wiart, Marlène Nanotheranostics Research Paper Background: The objective of this study was to demonstrate that synchrotron K-edge subtraction tomography (SKES-CT) can simultaneously track therapeutic cells and their encapsulating carrier, in vivo, in a rat model of focal brain injury using a dual-contrast agent approach. The second objective was to determine if SKES-CT could be used as a reference method for spectral photon counting tomography (SPCCT). Methods: Phantoms containing different concentrations of gold and iodine nanoparticles (AuNPS/INPs) were imaged with SKES-CT and SPCCT to assess their performances. A pre-clinical study was performed in rats with focal cerebral injury which intracerebrally received AuNPs-labelled therapeutic cells encapsulated in a INPs-labelled scaffold. Animals were imaged in vivo with SKES-CT and back-to-back with SPCCT. Results: SKES-CT revealed to be reliable for quantification of gold and iodine, whether alone or mixed. In the preclinical model, SKES-CT showed that AuNPs remained at the site of cell injection, while INPs expanded within and/or along the lesion border, suggesting dissociation of both components in the first days post-administration. Compared to SKES-CT, SPCCT was able to correctly locate gold, but not completely located iodine. When SKES-CT was used as reference, SPCCT gold quantification appeared very accurate both in vitro and in vivo. Iodine quantification by SPCCT was also quite accurate, albeit less so than for gold. Conclusion: We here provide the proof-of-concept that SKES-CT is a novel method of choice for performing dual-contrast agent imaging in the context of brain regenerative therapy. SKES-CT may also serve as ground truth for emerging technologies such as multicolour clinical SPCCT. Ivyspring International Publisher 2023-01-16 /pmc/articles/PMC9925349/ /pubmed/36793350 http://dx.doi.org/10.7150/ntno.79575 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Tavakoli, Clément
Cuccione, Elisa
Dumot, Chloé
Balegamire, Joëlle
Si-Mohamed, Salim Aymeric
Kim, Johoon
Crola-da-Silva, Claire
Chevalier, Yves
Boussel, Loïc
Douek, Philippe
Cormode, David
Elleaume, Hélène
Brun, Emmanuel
Wiart, Marlène
High-resolution synchrotron K-edge subtraction CT allows tracking and quantifying therapeutic cells and their scaffold in a rat model of focal cerebral injury and can serve as a reference for spectral photon counting CT
title High-resolution synchrotron K-edge subtraction CT allows tracking and quantifying therapeutic cells and their scaffold in a rat model of focal cerebral injury and can serve as a reference for spectral photon counting CT
title_full High-resolution synchrotron K-edge subtraction CT allows tracking and quantifying therapeutic cells and their scaffold in a rat model of focal cerebral injury and can serve as a reference for spectral photon counting CT
title_fullStr High-resolution synchrotron K-edge subtraction CT allows tracking and quantifying therapeutic cells and their scaffold in a rat model of focal cerebral injury and can serve as a reference for spectral photon counting CT
title_full_unstemmed High-resolution synchrotron K-edge subtraction CT allows tracking and quantifying therapeutic cells and their scaffold in a rat model of focal cerebral injury and can serve as a reference for spectral photon counting CT
title_short High-resolution synchrotron K-edge subtraction CT allows tracking and quantifying therapeutic cells and their scaffold in a rat model of focal cerebral injury and can serve as a reference for spectral photon counting CT
title_sort high-resolution synchrotron k-edge subtraction ct allows tracking and quantifying therapeutic cells and their scaffold in a rat model of focal cerebral injury and can serve as a reference for spectral photon counting ct
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9925349/
https://www.ncbi.nlm.nih.gov/pubmed/36793350
http://dx.doi.org/10.7150/ntno.79575
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