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Pseudomonas syringae DC3000 infection increases glucosylated N-glycans in Arabidopsis thaliana
Studying the interaction between the hemibiotrophic bacterium Pseudomonas syringae pv. tomato DC3000 and Arabidopsis thaliana has shed light onto the various forms of mechanisms plants use to defend themselves against pathogen attack. While a lot of emphasis has been put on investigating changes in...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9925501/ https://www.ncbi.nlm.nih.gov/pubmed/36269466 http://dx.doi.org/10.1007/s10719-022-10084-6 |
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author | Beihammer, Gernot Romero-Pérez, Andrea Maresch, Daniel Figl, Rudolf Mócsai, Réka Grünwald-Gruber, Clemens Altmann, Friedrich Van Damme, Els J. M. Strasser, Richard |
author_facet | Beihammer, Gernot Romero-Pérez, Andrea Maresch, Daniel Figl, Rudolf Mócsai, Réka Grünwald-Gruber, Clemens Altmann, Friedrich Van Damme, Els J. M. Strasser, Richard |
author_sort | Beihammer, Gernot |
collection | PubMed |
description | Studying the interaction between the hemibiotrophic bacterium Pseudomonas syringae pv. tomato DC3000 and Arabidopsis thaliana has shed light onto the various forms of mechanisms plants use to defend themselves against pathogen attack. While a lot of emphasis has been put on investigating changes in protein expression in infected plants, only little information is available on the effect infection plays on the plants N-glycan composition. To close this gap in knowledge, total N-glycans were enriched from P. syringae DC3000-infected and mock treated Arabidopsis seedlings and analyzed via MALDI-TOF–MS. Additionally, fluorescently labelled N-glycans were quantified via HPLC-FLD. N-glycans from infected plants were overall less processed and displayed increased amounts of oligomannosidic N-glycans. As multiple peaks for certain oligomannosidic glycoforms were detected upon separation via liquid chromatography, a porous graphitic carbon (PGC)-analysis was conducted to separate individual N-glycan isomers. Indeed, multiple different N-glycan isomers with masses of two N-acetylhexosamine residues plus 8, 9 or 10 hexoses were detected in the infected plants which were absent in the mock controls. Treatment with jack bean α-mannosidase resulted in incomplete removal of hexoses from these N-glycans, indicating the presence of glucose residues. This hints at the accumulation of misfolded glycoproteins in the infected plants, likely because of endoplasmic reticulum (ER) stress. In addition, poly-hexose structures susceptible to α-amylase treatment were found in the DC3000-infected plants, indicating alterations in starch metabolism due to the infection process. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10719-022-10084-6. |
format | Online Article Text |
id | pubmed-9925501 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-99255012023-02-15 Pseudomonas syringae DC3000 infection increases glucosylated N-glycans in Arabidopsis thaliana Beihammer, Gernot Romero-Pérez, Andrea Maresch, Daniel Figl, Rudolf Mócsai, Réka Grünwald-Gruber, Clemens Altmann, Friedrich Van Damme, Els J. M. Strasser, Richard Glycoconj J Original Article Studying the interaction between the hemibiotrophic bacterium Pseudomonas syringae pv. tomato DC3000 and Arabidopsis thaliana has shed light onto the various forms of mechanisms plants use to defend themselves against pathogen attack. While a lot of emphasis has been put on investigating changes in protein expression in infected plants, only little information is available on the effect infection plays on the plants N-glycan composition. To close this gap in knowledge, total N-glycans were enriched from P. syringae DC3000-infected and mock treated Arabidopsis seedlings and analyzed via MALDI-TOF–MS. Additionally, fluorescently labelled N-glycans were quantified via HPLC-FLD. N-glycans from infected plants were overall less processed and displayed increased amounts of oligomannosidic N-glycans. As multiple peaks for certain oligomannosidic glycoforms were detected upon separation via liquid chromatography, a porous graphitic carbon (PGC)-analysis was conducted to separate individual N-glycan isomers. Indeed, multiple different N-glycan isomers with masses of two N-acetylhexosamine residues plus 8, 9 or 10 hexoses were detected in the infected plants which were absent in the mock controls. Treatment with jack bean α-mannosidase resulted in incomplete removal of hexoses from these N-glycans, indicating the presence of glucose residues. This hints at the accumulation of misfolded glycoproteins in the infected plants, likely because of endoplasmic reticulum (ER) stress. In addition, poly-hexose structures susceptible to α-amylase treatment were found in the DC3000-infected plants, indicating alterations in starch metabolism due to the infection process. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10719-022-10084-6. Springer US 2022-10-21 2023 /pmc/articles/PMC9925501/ /pubmed/36269466 http://dx.doi.org/10.1007/s10719-022-10084-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Beihammer, Gernot Romero-Pérez, Andrea Maresch, Daniel Figl, Rudolf Mócsai, Réka Grünwald-Gruber, Clemens Altmann, Friedrich Van Damme, Els J. M. Strasser, Richard Pseudomonas syringae DC3000 infection increases glucosylated N-glycans in Arabidopsis thaliana |
title | Pseudomonas syringae DC3000 infection increases glucosylated N-glycans in Arabidopsis thaliana |
title_full | Pseudomonas syringae DC3000 infection increases glucosylated N-glycans in Arabidopsis thaliana |
title_fullStr | Pseudomonas syringae DC3000 infection increases glucosylated N-glycans in Arabidopsis thaliana |
title_full_unstemmed | Pseudomonas syringae DC3000 infection increases glucosylated N-glycans in Arabidopsis thaliana |
title_short | Pseudomonas syringae DC3000 infection increases glucosylated N-glycans in Arabidopsis thaliana |
title_sort | pseudomonas syringae dc3000 infection increases glucosylated n-glycans in arabidopsis thaliana |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9925501/ https://www.ncbi.nlm.nih.gov/pubmed/36269466 http://dx.doi.org/10.1007/s10719-022-10084-6 |
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