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Phosphorylated chitosan accelerates dermal wound healing in diabetic wistar rats

Phosphorylated chitosan (PC), a water-soluble derivative of chitosan possesses several biological and chemical properties suitable for diabetic wound healing. In the present study, we report the synthesis and diabetic wound healing capabilities of PC. Elemental analysis, FT-IR, (13)C-NMR and (31)P-N...

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Autores principales: Anushree, U., Punj, Pratik, Vasumathi, Bharati, Sanjay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9925528/
https://www.ncbi.nlm.nih.gov/pubmed/36447107
http://dx.doi.org/10.1007/s10719-022-10093-5
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author Anushree, U.
Punj, Pratik
Vasumathi
Bharati, Sanjay
author_facet Anushree, U.
Punj, Pratik
Vasumathi
Bharati, Sanjay
author_sort Anushree, U.
collection PubMed
description Phosphorylated chitosan (PC), a water-soluble derivative of chitosan possesses several biological and chemical properties suitable for diabetic wound healing. In the present study, we report the synthesis and diabetic wound healing capabilities of PC. Elemental analysis, FT-IR, (13)C-NMR and (31)P-NMR techniques were employed for the chemical characterization of PC. In vitro, antioxidant properties of PC were determined in terms of Fe(3+) reducing, metal chelating, lipid peroxidation and superoxide scavenging ability. The wound healing potential of PC was assessed in diabetic excisional wound rat model. PC exhibited good water solubility, and in vitro antioxidant capacity. Wound contraction was higher in PC-treated wounds (91.11%) as compared to untreated wounds (67.26%) on 14(th)-day post wound creation. Histopathology of PC-treated wounds revealed improved tissue morphology with higher number of fibroblasts, a thicker epithelial layer, enhanced collagen deposits and angiogenesis as compared to untreated wounds. An overall increase of 57% and 25% in hydroxylamine and hexosamine content respectively were noted as compared to untreated wounds. A significant (P ≤ 0.05) increase in SOD activity and a significant (P ≤ 0.05) decrease in lipid peroxides were recorded in PC-treated wounds as compared to untreated wounds. These observations demonstrated that PC can be used as an effective agent in diabetic wound healing. GRAPHICAL ABSTRACT: Illustration of phosphorylated chitosan (PC) synthesis and its wound healing potential: Chitosan was phosphorylated to impart diabetic wound healing properties. Chemical characterizations such as elemental analysis, FT-IR and NMR confirmed successful phosphorylation of chitosan. PC exhibited good in vitro antioxidant properties. To assess the diabetic wound healing potential, an excisional wound model was developed in diabetic rats. PC treatment demonstrated accelerated wound healing. [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10719-022-10093-5.
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spelling pubmed-99255282023-02-15 Phosphorylated chitosan accelerates dermal wound healing in diabetic wistar rats Anushree, U. Punj, Pratik Vasumathi Bharati, Sanjay Glycoconj J Original Article Phosphorylated chitosan (PC), a water-soluble derivative of chitosan possesses several biological and chemical properties suitable for diabetic wound healing. In the present study, we report the synthesis and diabetic wound healing capabilities of PC. Elemental analysis, FT-IR, (13)C-NMR and (31)P-NMR techniques were employed for the chemical characterization of PC. In vitro, antioxidant properties of PC were determined in terms of Fe(3+) reducing, metal chelating, lipid peroxidation and superoxide scavenging ability. The wound healing potential of PC was assessed in diabetic excisional wound rat model. PC exhibited good water solubility, and in vitro antioxidant capacity. Wound contraction was higher in PC-treated wounds (91.11%) as compared to untreated wounds (67.26%) on 14(th)-day post wound creation. Histopathology of PC-treated wounds revealed improved tissue morphology with higher number of fibroblasts, a thicker epithelial layer, enhanced collagen deposits and angiogenesis as compared to untreated wounds. An overall increase of 57% and 25% in hydroxylamine and hexosamine content respectively were noted as compared to untreated wounds. A significant (P ≤ 0.05) increase in SOD activity and a significant (P ≤ 0.05) decrease in lipid peroxides were recorded in PC-treated wounds as compared to untreated wounds. These observations demonstrated that PC can be used as an effective agent in diabetic wound healing. GRAPHICAL ABSTRACT: Illustration of phosphorylated chitosan (PC) synthesis and its wound healing potential: Chitosan was phosphorylated to impart diabetic wound healing properties. Chemical characterizations such as elemental analysis, FT-IR and NMR confirmed successful phosphorylation of chitosan. PC exhibited good in vitro antioxidant properties. To assess the diabetic wound healing potential, an excisional wound model was developed in diabetic rats. PC treatment demonstrated accelerated wound healing. [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10719-022-10093-5. Springer US 2022-11-30 2023 /pmc/articles/PMC9925528/ /pubmed/36447107 http://dx.doi.org/10.1007/s10719-022-10093-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Anushree, U.
Punj, Pratik
Vasumathi
Bharati, Sanjay
Phosphorylated chitosan accelerates dermal wound healing in diabetic wistar rats
title Phosphorylated chitosan accelerates dermal wound healing in diabetic wistar rats
title_full Phosphorylated chitosan accelerates dermal wound healing in diabetic wistar rats
title_fullStr Phosphorylated chitosan accelerates dermal wound healing in diabetic wistar rats
title_full_unstemmed Phosphorylated chitosan accelerates dermal wound healing in diabetic wistar rats
title_short Phosphorylated chitosan accelerates dermal wound healing in diabetic wistar rats
title_sort phosphorylated chitosan accelerates dermal wound healing in diabetic wistar rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9925528/
https://www.ncbi.nlm.nih.gov/pubmed/36447107
http://dx.doi.org/10.1007/s10719-022-10093-5
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