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Effect of MAOA DNA Methylation on Human in Vivo Protein Expression Measured by [(11)C]harmine Positron Emission Tomography

BACKGROUND: Epigenetic modifications like DNA methylation are understood as an intermediary between environmental factors and neurobiology. Cerebral monoamine oxidase A (MAO-A) levels are altered in depression, as are DNA methylation levels within the MAOA gene, particularly in the promoter/exon I/i...

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Autores principales: Handschuh, Patricia A, Murgaš, Matej, Vraka, Chrysoula, Nics, Lukas, Hartmann, Annette M, Winkler-Pjrek, Edda, Baldinger-Melich, Pia, Wadsak, Wolfgang, Winkler, Dietmar, Hacker, Marcus, Rujescu, Dan, Domschke, Katharina, Lanzenberger, Rupert, Spies, Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9926052/
https://www.ncbi.nlm.nih.gov/pubmed/36573644
http://dx.doi.org/10.1093/ijnp/pyac085
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author Handschuh, Patricia A
Murgaš, Matej
Vraka, Chrysoula
Nics, Lukas
Hartmann, Annette M
Winkler-Pjrek, Edda
Baldinger-Melich, Pia
Wadsak, Wolfgang
Winkler, Dietmar
Hacker, Marcus
Rujescu, Dan
Domschke, Katharina
Lanzenberger, Rupert
Spies, Marie
author_facet Handschuh, Patricia A
Murgaš, Matej
Vraka, Chrysoula
Nics, Lukas
Hartmann, Annette M
Winkler-Pjrek, Edda
Baldinger-Melich, Pia
Wadsak, Wolfgang
Winkler, Dietmar
Hacker, Marcus
Rujescu, Dan
Domschke, Katharina
Lanzenberger, Rupert
Spies, Marie
author_sort Handschuh, Patricia A
collection PubMed
description BACKGROUND: Epigenetic modifications like DNA methylation are understood as an intermediary between environmental factors and neurobiology. Cerebral monoamine oxidase A (MAO-A) levels are altered in depression, as are DNA methylation levels within the MAOA gene, particularly in the promoter/exon I/intron I region. An effect of MAOA methylation on peripheral protein expression was shown, but the extent to which methylation affects brain MAO-A levels is not fully understood. METHODS: Here, the influence of MAOA promoter/exon I/intron I region DNA methylation on global MAO-A distribution volume (V(T)), an index of MAO-A density, was assessed via [(11)C]harmine positron emission tomography in 22 patients (14 females) suffering from seasonal affective disorder and 30 healthy controls (17 females). RESULTS: No significant influence of MAOA DNA methylation on global MAO-A V(T) was found, despite correction for health status, sex, season, and MAOA variable number of tandem repeat genotype. However, season affected average methylation in women, with higher levels in spring and summer (P(uncorr) = .03). We thus did not find evidence for an effect of MAOA DNA methylation on brain MAO-A V(T). CONCLUSIONS: In contrast to a previous study demonstrating an effect of methylation of a MAOA promoter region located further 5’ on brain MAO-A, MAOA methylation of the region assessed here appears to affect brain protein levels to a limited extent at most. The observed effect of season on methylation levels is in accordance with extensive evidence for seasonal effects within the serotonergic system. CLINICALTRIALS.GOV IDENTIFIER: NCT02582398 (https://clinicaltrials.gov/ct2/show/NCT02582398).
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spelling pubmed-99260522023-02-14 Effect of MAOA DNA Methylation on Human in Vivo Protein Expression Measured by [(11)C]harmine Positron Emission Tomography Handschuh, Patricia A Murgaš, Matej Vraka, Chrysoula Nics, Lukas Hartmann, Annette M Winkler-Pjrek, Edda Baldinger-Melich, Pia Wadsak, Wolfgang Winkler, Dietmar Hacker, Marcus Rujescu, Dan Domschke, Katharina Lanzenberger, Rupert Spies, Marie Int J Neuropsychopharmacol Regular Research Articles BACKGROUND: Epigenetic modifications like DNA methylation are understood as an intermediary between environmental factors and neurobiology. Cerebral monoamine oxidase A (MAO-A) levels are altered in depression, as are DNA methylation levels within the MAOA gene, particularly in the promoter/exon I/intron I region. An effect of MAOA methylation on peripheral protein expression was shown, but the extent to which methylation affects brain MAO-A levels is not fully understood. METHODS: Here, the influence of MAOA promoter/exon I/intron I region DNA methylation on global MAO-A distribution volume (V(T)), an index of MAO-A density, was assessed via [(11)C]harmine positron emission tomography in 22 patients (14 females) suffering from seasonal affective disorder and 30 healthy controls (17 females). RESULTS: No significant influence of MAOA DNA methylation on global MAO-A V(T) was found, despite correction for health status, sex, season, and MAOA variable number of tandem repeat genotype. However, season affected average methylation in women, with higher levels in spring and summer (P(uncorr) = .03). We thus did not find evidence for an effect of MAOA DNA methylation on brain MAO-A V(T). CONCLUSIONS: In contrast to a previous study demonstrating an effect of methylation of a MAOA promoter region located further 5’ on brain MAO-A, MAOA methylation of the region assessed here appears to affect brain protein levels to a limited extent at most. The observed effect of season on methylation levels is in accordance with extensive evidence for seasonal effects within the serotonergic system. CLINICALTRIALS.GOV IDENTIFIER: NCT02582398 (https://clinicaltrials.gov/ct2/show/NCT02582398). Oxford University Press 2022-12-27 /pmc/articles/PMC9926052/ /pubmed/36573644 http://dx.doi.org/10.1093/ijnp/pyac085 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of CINP. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Regular Research Articles
Handschuh, Patricia A
Murgaš, Matej
Vraka, Chrysoula
Nics, Lukas
Hartmann, Annette M
Winkler-Pjrek, Edda
Baldinger-Melich, Pia
Wadsak, Wolfgang
Winkler, Dietmar
Hacker, Marcus
Rujescu, Dan
Domschke, Katharina
Lanzenberger, Rupert
Spies, Marie
Effect of MAOA DNA Methylation on Human in Vivo Protein Expression Measured by [(11)C]harmine Positron Emission Tomography
title Effect of MAOA DNA Methylation on Human in Vivo Protein Expression Measured by [(11)C]harmine Positron Emission Tomography
title_full Effect of MAOA DNA Methylation on Human in Vivo Protein Expression Measured by [(11)C]harmine Positron Emission Tomography
title_fullStr Effect of MAOA DNA Methylation on Human in Vivo Protein Expression Measured by [(11)C]harmine Positron Emission Tomography
title_full_unstemmed Effect of MAOA DNA Methylation on Human in Vivo Protein Expression Measured by [(11)C]harmine Positron Emission Tomography
title_short Effect of MAOA DNA Methylation on Human in Vivo Protein Expression Measured by [(11)C]harmine Positron Emission Tomography
title_sort effect of maoa dna methylation on human in vivo protein expression measured by [(11)c]harmine positron emission tomography
topic Regular Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9926052/
https://www.ncbi.nlm.nih.gov/pubmed/36573644
http://dx.doi.org/10.1093/ijnp/pyac085
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