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Rice DST transcription factor negatively regulates heat tolerance through ROS-mediated stomatal movement and heat-responsive gene expression

Plants are frequently subjected to a broad spectrum of abiotic stresses including drought, salinity and extreme temperatures and have evolved both common and stress-specific responses to promote fitness and survival. Understanding the components and mechanisms that underlie both common and stress-sp...

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Autores principales: Ding, Yanfei, Zhou, Mei, Wang, Ke, Qu, Aili, Hu, Shanshan, Jiang, Qiong, Yi, Keke, Wang, Feijuan, Cai, Chong, Zhu, Cheng, Chen, Zhixiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9927019/
https://www.ncbi.nlm.nih.gov/pubmed/36798712
http://dx.doi.org/10.3389/fpls.2023.1068296
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author Ding, Yanfei
Zhou, Mei
Wang, Ke
Qu, Aili
Hu, Shanshan
Jiang, Qiong
Yi, Keke
Wang, Feijuan
Cai, Chong
Zhu, Cheng
Chen, Zhixiang
author_facet Ding, Yanfei
Zhou, Mei
Wang, Ke
Qu, Aili
Hu, Shanshan
Jiang, Qiong
Yi, Keke
Wang, Feijuan
Cai, Chong
Zhu, Cheng
Chen, Zhixiang
author_sort Ding, Yanfei
collection PubMed
description Plants are frequently subjected to a broad spectrum of abiotic stresses including drought, salinity and extreme temperatures and have evolved both common and stress-specific responses to promote fitness and survival. Understanding the components and mechanisms that underlie both common and stress-specific responses can enable development of crop plants tolerant to different stresses. Here, we report a rice heat stress-tolerant 1 (hst1) mutant with increased heat tolerance. HST1 encodes the DST transcription factor, which also regulates drought and salinity tolerance. Increased heat tolerance of hst1 was associated with suppressed expression of reactive oxygen species (ROS)-scavenging peroxidases and increased ROS levels, which reduced water loss by decreasing stomatal aperture under heat stress. In addition, increased ROS levels enhanced expression of genes encoding heat shock protein (HSPs) including HSP80, HSP74, HSP58 and small HSPs. HSPs promote stabilization of proteins and protein refolding under heat stress and accordingly mutation of HST1 also improved reproductive traits including pollen viability and seed setting under high temperature. These results broaden the negative roles of DST in abiotic stress tolerance and provide important new insights into DST-regulated tolerance to diverse abiotic stresses through both shared and stress-specific mechanisms.
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spelling pubmed-99270192023-02-15 Rice DST transcription factor negatively regulates heat tolerance through ROS-mediated stomatal movement and heat-responsive gene expression Ding, Yanfei Zhou, Mei Wang, Ke Qu, Aili Hu, Shanshan Jiang, Qiong Yi, Keke Wang, Feijuan Cai, Chong Zhu, Cheng Chen, Zhixiang Front Plant Sci Plant Science Plants are frequently subjected to a broad spectrum of abiotic stresses including drought, salinity and extreme temperatures and have evolved both common and stress-specific responses to promote fitness and survival. Understanding the components and mechanisms that underlie both common and stress-specific responses can enable development of crop plants tolerant to different stresses. Here, we report a rice heat stress-tolerant 1 (hst1) mutant with increased heat tolerance. HST1 encodes the DST transcription factor, which also regulates drought and salinity tolerance. Increased heat tolerance of hst1 was associated with suppressed expression of reactive oxygen species (ROS)-scavenging peroxidases and increased ROS levels, which reduced water loss by decreasing stomatal aperture under heat stress. In addition, increased ROS levels enhanced expression of genes encoding heat shock protein (HSPs) including HSP80, HSP74, HSP58 and small HSPs. HSPs promote stabilization of proteins and protein refolding under heat stress and accordingly mutation of HST1 also improved reproductive traits including pollen viability and seed setting under high temperature. These results broaden the negative roles of DST in abiotic stress tolerance and provide important new insights into DST-regulated tolerance to diverse abiotic stresses through both shared and stress-specific mechanisms. Frontiers Media S.A. 2023-01-31 /pmc/articles/PMC9927019/ /pubmed/36798712 http://dx.doi.org/10.3389/fpls.2023.1068296 Text en Copyright © 2023 Ding, Zhou, Wang, Qu, Hu, Jiang, Yi, Wang, Cai, Zhu and Chen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ding, Yanfei
Zhou, Mei
Wang, Ke
Qu, Aili
Hu, Shanshan
Jiang, Qiong
Yi, Keke
Wang, Feijuan
Cai, Chong
Zhu, Cheng
Chen, Zhixiang
Rice DST transcription factor negatively regulates heat tolerance through ROS-mediated stomatal movement and heat-responsive gene expression
title Rice DST transcription factor negatively regulates heat tolerance through ROS-mediated stomatal movement and heat-responsive gene expression
title_full Rice DST transcription factor negatively regulates heat tolerance through ROS-mediated stomatal movement and heat-responsive gene expression
title_fullStr Rice DST transcription factor negatively regulates heat tolerance through ROS-mediated stomatal movement and heat-responsive gene expression
title_full_unstemmed Rice DST transcription factor negatively regulates heat tolerance through ROS-mediated stomatal movement and heat-responsive gene expression
title_short Rice DST transcription factor negatively regulates heat tolerance through ROS-mediated stomatal movement and heat-responsive gene expression
title_sort rice dst transcription factor negatively regulates heat tolerance through ros-mediated stomatal movement and heat-responsive gene expression
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9927019/
https://www.ncbi.nlm.nih.gov/pubmed/36798712
http://dx.doi.org/10.3389/fpls.2023.1068296
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