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Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice
Development of SARS-CoV-2 vaccines that protect vulnerable populations is a public health priority. Here, we took a systematic and iterative approach by testing several adjuvants and SARS-CoV-2 antigens to identify a combination that elicits antibodies and protection in young and aged mice. While de...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9927065/ https://www.ncbi.nlm.nih.gov/pubmed/36788219 http://dx.doi.org/10.1038/s41541-023-00610-4 |
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| author | Nanishi, Etsuro Borriello, Francesco Seo, Hyuk-Soo O’Meara, Timothy R. McGrath, Marisa E. Saito, Yoshine Chen, Jing Diray-Arce, Joann Song, Kijun Xu, Andrew Z. Barman, Soumik Menon, Manisha Dong, Danica Caradonna, Timothy M. Feldman, Jared Hauser, Blake M. Schmidt, Aaron G. Baden, Lindsey R. Ernst, Robert K. Dillen, Carly Yu, Jingyou Chang, Aiquan Hilgers, Luuk Platenburg, Peter Paul Dhe-Paganon, Sirano Barouch, Dan H. Ozonoff, Al Zanoni, Ivan Frieman, Matthew B. Dowling, David J. Levy, Ofer |
| author_facet | Nanishi, Etsuro Borriello, Francesco Seo, Hyuk-Soo O’Meara, Timothy R. McGrath, Marisa E. Saito, Yoshine Chen, Jing Diray-Arce, Joann Song, Kijun Xu, Andrew Z. Barman, Soumik Menon, Manisha Dong, Danica Caradonna, Timothy M. Feldman, Jared Hauser, Blake M. Schmidt, Aaron G. Baden, Lindsey R. Ernst, Robert K. Dillen, Carly Yu, Jingyou Chang, Aiquan Hilgers, Luuk Platenburg, Peter Paul Dhe-Paganon, Sirano Barouch, Dan H. Ozonoff, Al Zanoni, Ivan Frieman, Matthew B. Dowling, David J. Levy, Ofer |
| author_sort | Nanishi, Etsuro |
| collection | PubMed |
| description | Development of SARS-CoV-2 vaccines that protect vulnerable populations is a public health priority. Here, we took a systematic and iterative approach by testing several adjuvants and SARS-CoV-2 antigens to identify a combination that elicits antibodies and protection in young and aged mice. While demonstrating superior immunogenicity to soluble receptor-binding domain (RBD), RBD displayed as a protein nanoparticle (RBD-NP) generated limited antibody responses. Comparison of multiple adjuvants including AddaVax, AddaS03, and AS01B in young and aged mice demonstrated that an oil-in-water emulsion containing carbohydrate fatty acid monosulphate derivative (CMS:O/W) most effectively enhanced RBD-NP-induced cross-neutralizing antibodies and protection across age groups. CMS:O/W enhanced antigen retention in the draining lymph node, induced injection site, and lymph node cytokines, with CMS inducing MyD88-dependent Th1 cytokine polarization. Furthermore, CMS and O/W synergistically induced chemokine production from human PBMCs. Overall, CMS:O/W adjuvant may enhance immunogenicity and protection of vulnerable populations against SARS-CoV-2 and other infectious pathogens. |
| format | Online Article Text |
| id | pubmed-9927065 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-99270652023-02-15 Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice Nanishi, Etsuro Borriello, Francesco Seo, Hyuk-Soo O’Meara, Timothy R. McGrath, Marisa E. Saito, Yoshine Chen, Jing Diray-Arce, Joann Song, Kijun Xu, Andrew Z. Barman, Soumik Menon, Manisha Dong, Danica Caradonna, Timothy M. Feldman, Jared Hauser, Blake M. Schmidt, Aaron G. Baden, Lindsey R. Ernst, Robert K. Dillen, Carly Yu, Jingyou Chang, Aiquan Hilgers, Luuk Platenburg, Peter Paul Dhe-Paganon, Sirano Barouch, Dan H. Ozonoff, Al Zanoni, Ivan Frieman, Matthew B. Dowling, David J. Levy, Ofer NPJ Vaccines Article Development of SARS-CoV-2 vaccines that protect vulnerable populations is a public health priority. Here, we took a systematic and iterative approach by testing several adjuvants and SARS-CoV-2 antigens to identify a combination that elicits antibodies and protection in young and aged mice. While demonstrating superior immunogenicity to soluble receptor-binding domain (RBD), RBD displayed as a protein nanoparticle (RBD-NP) generated limited antibody responses. Comparison of multiple adjuvants including AddaVax, AddaS03, and AS01B in young and aged mice demonstrated that an oil-in-water emulsion containing carbohydrate fatty acid monosulphate derivative (CMS:O/W) most effectively enhanced RBD-NP-induced cross-neutralizing antibodies and protection across age groups. CMS:O/W enhanced antigen retention in the draining lymph node, induced injection site, and lymph node cytokines, with CMS inducing MyD88-dependent Th1 cytokine polarization. Furthermore, CMS and O/W synergistically induced chemokine production from human PBMCs. Overall, CMS:O/W adjuvant may enhance immunogenicity and protection of vulnerable populations against SARS-CoV-2 and other infectious pathogens. Nature Publishing Group UK 2023-02-14 /pmc/articles/PMC9927065/ /pubmed/36788219 http://dx.doi.org/10.1038/s41541-023-00610-4 Text en © The Author(s) 2023, corrected publication 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
| spellingShingle | Article Nanishi, Etsuro Borriello, Francesco Seo, Hyuk-Soo O’Meara, Timothy R. McGrath, Marisa E. Saito, Yoshine Chen, Jing Diray-Arce, Joann Song, Kijun Xu, Andrew Z. Barman, Soumik Menon, Manisha Dong, Danica Caradonna, Timothy M. Feldman, Jared Hauser, Blake M. Schmidt, Aaron G. Baden, Lindsey R. Ernst, Robert K. Dillen, Carly Yu, Jingyou Chang, Aiquan Hilgers, Luuk Platenburg, Peter Paul Dhe-Paganon, Sirano Barouch, Dan H. Ozonoff, Al Zanoni, Ivan Frieman, Matthew B. Dowling, David J. Levy, Ofer Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice |
| title | Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice |
| title_full | Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice |
| title_fullStr | Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice |
| title_full_unstemmed | Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice |
| title_short | Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice |
| title_sort | carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances sars-cov-2 rbd nanoparticle-induced immunogenicity and protection in mice |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9927065/ https://www.ncbi.nlm.nih.gov/pubmed/36788219 http://dx.doi.org/10.1038/s41541-023-00610-4 |
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