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Antigen B modulates anti‐inflammatory cytokines in the EAE model of multiple sclerosis
INTRODUCTION: : Multiple sclerosis (MS) is characterized by the destruction of the blood–brain barrier, loss of myelin sheath, and contribution of inflammatory interleukins such as TNF‐alpha, interleukin‐17, and interleukin‐6. METHODS: : The current study investigated the effect of antigen B of hyda...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9927863/ https://www.ncbi.nlm.nih.gov/pubmed/36582052 http://dx.doi.org/10.1002/brb3.2874 |
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author | Mariki, Aliakbar Barzin, Zahra Fasihi Harandi, Majid Karbasi Ravari, Kimia Davoodi, Mahboubeh Mousavi, Seyed Mohammad Rezakhani, Soheila Nazeri, Masoud Shabani, Mohammad |
author_facet | Mariki, Aliakbar Barzin, Zahra Fasihi Harandi, Majid Karbasi Ravari, Kimia Davoodi, Mahboubeh Mousavi, Seyed Mohammad Rezakhani, Soheila Nazeri, Masoud Shabani, Mohammad |
author_sort | Mariki, Aliakbar |
collection | PubMed |
description | INTRODUCTION: : Multiple sclerosis (MS) is characterized by the destruction of the blood–brain barrier, loss of myelin sheath, and contribution of inflammatory interleukins such as TNF‐alpha, interleukin‐17, and interleukin‐6. METHODS: : The current study investigated the effect of antigen B of hydatid cyst fluid on the reduction of anti‐inflammatory cytokines and nerve conduction velocity in rats with experimental autoimmune encephalomyelitis (EAE)‐induced MS. After isolation of antigen B from sterile cyst fluid, the rats were randomly divided into four groups: saline, EAE, EAE + teriflunomide (EAE + TF), and EAE + antigen B (EAE + AngB). The EAE model was induced using cow spinal cord homogenization, in combination with Freund's complete adjuvant. The serum concentration of cytokines including IL‐1B and IL‐17, IL‐10, IL‐6, and TNF‐X was measured by the ELISA method, and real‐time PCR was performed to study gene expression. Electrophysiological, behavioral, and neuropathological tests were also conducted. RESULTS: : Nerve conduction velocity and IL‐10 concentration were increased in the antigen B group. The results of this study showed that antigen B reduced the inflammatory component of the EAE MS animal model by modulating the immune system compared to teriflunomide, which eventually led to a reduction in symptoms at the behavioral and electrophysiological level. CONCLUSIONS: : It seems that antigen B plays a critical role in regulating immunity and it can be used as a possible therapeutic agent to modulate the immune system in MS patients. It might be rational to consider hydatid cyst fluid antigen as a modifier in MS. |
format | Online Article Text |
id | pubmed-9927863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99278632023-02-16 Antigen B modulates anti‐inflammatory cytokines in the EAE model of multiple sclerosis Mariki, Aliakbar Barzin, Zahra Fasihi Harandi, Majid Karbasi Ravari, Kimia Davoodi, Mahboubeh Mousavi, Seyed Mohammad Rezakhani, Soheila Nazeri, Masoud Shabani, Mohammad Brain Behav Original Articles INTRODUCTION: : Multiple sclerosis (MS) is characterized by the destruction of the blood–brain barrier, loss of myelin sheath, and contribution of inflammatory interleukins such as TNF‐alpha, interleukin‐17, and interleukin‐6. METHODS: : The current study investigated the effect of antigen B of hydatid cyst fluid on the reduction of anti‐inflammatory cytokines and nerve conduction velocity in rats with experimental autoimmune encephalomyelitis (EAE)‐induced MS. After isolation of antigen B from sterile cyst fluid, the rats were randomly divided into four groups: saline, EAE, EAE + teriflunomide (EAE + TF), and EAE + antigen B (EAE + AngB). The EAE model was induced using cow spinal cord homogenization, in combination with Freund's complete adjuvant. The serum concentration of cytokines including IL‐1B and IL‐17, IL‐10, IL‐6, and TNF‐X was measured by the ELISA method, and real‐time PCR was performed to study gene expression. Electrophysiological, behavioral, and neuropathological tests were also conducted. RESULTS: : Nerve conduction velocity and IL‐10 concentration were increased in the antigen B group. The results of this study showed that antigen B reduced the inflammatory component of the EAE MS animal model by modulating the immune system compared to teriflunomide, which eventually led to a reduction in symptoms at the behavioral and electrophysiological level. CONCLUSIONS: : It seems that antigen B plays a critical role in regulating immunity and it can be used as a possible therapeutic agent to modulate the immune system in MS patients. It might be rational to consider hydatid cyst fluid antigen as a modifier in MS. John Wiley and Sons Inc. 2022-12-29 /pmc/articles/PMC9927863/ /pubmed/36582052 http://dx.doi.org/10.1002/brb3.2874 Text en © 2022 The Authors. Brain and Behavior published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Mariki, Aliakbar Barzin, Zahra Fasihi Harandi, Majid Karbasi Ravari, Kimia Davoodi, Mahboubeh Mousavi, Seyed Mohammad Rezakhani, Soheila Nazeri, Masoud Shabani, Mohammad Antigen B modulates anti‐inflammatory cytokines in the EAE model of multiple sclerosis |
title | Antigen B modulates anti‐inflammatory cytokines in the EAE model of multiple sclerosis |
title_full | Antigen B modulates anti‐inflammatory cytokines in the EAE model of multiple sclerosis |
title_fullStr | Antigen B modulates anti‐inflammatory cytokines in the EAE model of multiple sclerosis |
title_full_unstemmed | Antigen B modulates anti‐inflammatory cytokines in the EAE model of multiple sclerosis |
title_short | Antigen B modulates anti‐inflammatory cytokines in the EAE model of multiple sclerosis |
title_sort | antigen b modulates anti‐inflammatory cytokines in the eae model of multiple sclerosis |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9927863/ https://www.ncbi.nlm.nih.gov/pubmed/36582052 http://dx.doi.org/10.1002/brb3.2874 |
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