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Advancement and obstacles in microfluidics-based isolation of extracellular vesicles

There is a great need for techniques which enable reproducible separation of extracellular vesicles (EVs) from biofluids with high recovery, purity and throughput. The development of new techniques for isolation of EVs from minute sample volumes is instrumental in enabling EV-based biomarker profili...

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Autores principales: Havers, Megan, Broman, Axel, Lenshof, Andreas, Laurell, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9928917/
https://www.ncbi.nlm.nih.gov/pubmed/36284018
http://dx.doi.org/10.1007/s00216-022-04362-3
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author Havers, Megan
Broman, Axel
Lenshof, Andreas
Laurell, Thomas
author_facet Havers, Megan
Broman, Axel
Lenshof, Andreas
Laurell, Thomas
author_sort Havers, Megan
collection PubMed
description There is a great need for techniques which enable reproducible separation of extracellular vesicles (EVs) from biofluids with high recovery, purity and throughput. The development of new techniques for isolation of EVs from minute sample volumes is instrumental in enabling EV-based biomarker profiling in large biobank cohorts and paves the way to improved diagnostic profiles in precision medicine. Recent advances in microfluidics-based devices offer a toolbox for separating EVs from small sample volumes. Microfluidic devices that have been used in EV isolation utilise different fundamental principles and rely largely on benefits of scaling laws as the biofluid processing is miniaturised to chip level. Here, we review the progress in the practicality and performance of both passive devices (such as mechanical filtering and hydrodynamic focusing) and active devices (using magnetic, electric or acoustic fields). As it stands, many microfluidic devices isolate intact EV populations at higher purities than centrifugation, precipitation or size-exclusion chromatography. However, this comes at a cost. We address challenges (in particular low throughput, clogging risks and ability to process biofluids) and highlight the need for more improvements in microfluidic devices. Finally, we conclude that there is a need to refine and standardise these lab-on-a-chip techniques to meet the growing interest in the diagnostic and therapeutic value of purified EVs. GRAPHICAL ABSTRACT: [Image: see text]
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spelling pubmed-99289172023-02-16 Advancement and obstacles in microfluidics-based isolation of extracellular vesicles Havers, Megan Broman, Axel Lenshof, Andreas Laurell, Thomas Anal Bioanal Chem Review There is a great need for techniques which enable reproducible separation of extracellular vesicles (EVs) from biofluids with high recovery, purity and throughput. The development of new techniques for isolation of EVs from minute sample volumes is instrumental in enabling EV-based biomarker profiling in large biobank cohorts and paves the way to improved diagnostic profiles in precision medicine. Recent advances in microfluidics-based devices offer a toolbox for separating EVs from small sample volumes. Microfluidic devices that have been used in EV isolation utilise different fundamental principles and rely largely on benefits of scaling laws as the biofluid processing is miniaturised to chip level. Here, we review the progress in the practicality and performance of both passive devices (such as mechanical filtering and hydrodynamic focusing) and active devices (using magnetic, electric or acoustic fields). As it stands, many microfluidic devices isolate intact EV populations at higher purities than centrifugation, precipitation or size-exclusion chromatography. However, this comes at a cost. We address challenges (in particular low throughput, clogging risks and ability to process biofluids) and highlight the need for more improvements in microfluidic devices. Finally, we conclude that there is a need to refine and standardise these lab-on-a-chip techniques to meet the growing interest in the diagnostic and therapeutic value of purified EVs. GRAPHICAL ABSTRACT: [Image: see text] Springer Berlin Heidelberg 2022-10-26 2023 /pmc/articles/PMC9928917/ /pubmed/36284018 http://dx.doi.org/10.1007/s00216-022-04362-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review
Havers, Megan
Broman, Axel
Lenshof, Andreas
Laurell, Thomas
Advancement and obstacles in microfluidics-based isolation of extracellular vesicles
title Advancement and obstacles in microfluidics-based isolation of extracellular vesicles
title_full Advancement and obstacles in microfluidics-based isolation of extracellular vesicles
title_fullStr Advancement and obstacles in microfluidics-based isolation of extracellular vesicles
title_full_unstemmed Advancement and obstacles in microfluidics-based isolation of extracellular vesicles
title_short Advancement and obstacles in microfluidics-based isolation of extracellular vesicles
title_sort advancement and obstacles in microfluidics-based isolation of extracellular vesicles
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9928917/
https://www.ncbi.nlm.nih.gov/pubmed/36284018
http://dx.doi.org/10.1007/s00216-022-04362-3
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