Cargando…

Comprehensive investigating of mismatch repair genes (MMR) polymorphisms in participants with chronic hepatitis B virus infection

Background and aim: In this study, we focused on the relationship between single nucleotide polymorphisms in MMR genes and the occurrence and development of HBV infection. Materials and methods: A total of 3,128 participants were divided into five groups: negative control group (NeC), spontaneous cl...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Ning, Sun, Yucheng, Kong, Yanan, Jin, Yiyao, Yu, Fengxue, Liu, Lianfeng, Yang, Lei, Liu, Wenxuan, Gao, Xia, Liu, Dianwu, Zhang, Xiaolin, Li, Lu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9928949/
https://www.ncbi.nlm.nih.gov/pubmed/36816025
http://dx.doi.org/10.3389/fgene.2023.1077297
_version_ 1784888740895784960
author Ma, Ning
Sun, Yucheng
Kong, Yanan
Jin, Yiyao
Yu, Fengxue
Liu, Lianfeng
Yang, Lei
Liu, Wenxuan
Gao, Xia
Liu, Dianwu
Zhang, Xiaolin
Li, Lu
author_facet Ma, Ning
Sun, Yucheng
Kong, Yanan
Jin, Yiyao
Yu, Fengxue
Liu, Lianfeng
Yang, Lei
Liu, Wenxuan
Gao, Xia
Liu, Dianwu
Zhang, Xiaolin
Li, Lu
author_sort Ma, Ning
collection PubMed
description Background and aim: In this study, we focused on the relationship between single nucleotide polymorphisms in MMR genes and the occurrence and development of HBV infection. Materials and methods: A total of 3,128 participants were divided into five groups: negative control group (NeC), spontaneous clearance group (SC), chronic hepatitis B group (CHB), liver cirrhosis group (LC) and hepatocellular carcinoma group (HCC), CHB, liver cirrhosis and hepatocellular carcinoma constitute HLD. We conducted three case-control studies: NeC (840 cases) vs. HLD (1792 cases), SC (486 cases) vs. HLD (1792 cases) and CHB + LC (1,371 cases) vs. HCC (421 cases). 11 polymorphic loci in MLH1, MLH3, MSH5, PMS1 and PMS2 were involved in genotyping by Sequenom MassArray. The SNPStats performed Hardy-Weinberg equilibrium test. Linkage disequilibrium patterns were visualized using Haploview4.2. The GMDR (v0.9) was conducted to generalized multifactor dimension reduction analysis. The correlation, multiplicative interaction and additive interaction analyses were calculated by Logistic Regression through SPSS21.0. Matrix and programmed excel were also involved in the calculation of additive interaction. Results: In NeC vs. HLD group, MSH5-rs1150793(G) was a risk base to HBV susceptibility (nominal p = 0.002, OR = 1.346). We found multiplicative interaction between MLH1-rs1540354 (AA + AT) and PMS1-rs1233255 (AA) (nominal p = 0.024, OR = 1.240). There was additive interaction between PMS1-rs1233255 (AA) and PMS1-rs256554(CA + CC). In SC vs. HLD group, MLH1-rs1540354 (TT) was a risk genotype (nominal p < 0.05, OR>1). Through haplotype analysis, we found the linkage disequilibrium of three loci in MLH1. The results of GMDR showed the optimal five-locus model about the spontaneous clearance of HBV. In CHB + LC vs. HCC group, PMS2-rs12112229(A) was related to the cancerization of liver. Conclusion: We found rs1150793(G), rs1540354(T) and rs12112229(A) were significantly related to HBV susceptibility, spontaneous clearance of HBV and cancerization after infection, respectively.
format Online
Article
Text
id pubmed-9928949
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-99289492023-02-16 Comprehensive investigating of mismatch repair genes (MMR) polymorphisms in participants with chronic hepatitis B virus infection Ma, Ning Sun, Yucheng Kong, Yanan Jin, Yiyao Yu, Fengxue Liu, Lianfeng Yang, Lei Liu, Wenxuan Gao, Xia Liu, Dianwu Zhang, Xiaolin Li, Lu Front Genet Genetics Background and aim: In this study, we focused on the relationship between single nucleotide polymorphisms in MMR genes and the occurrence and development of HBV infection. Materials and methods: A total of 3,128 participants were divided into five groups: negative control group (NeC), spontaneous clearance group (SC), chronic hepatitis B group (CHB), liver cirrhosis group (LC) and hepatocellular carcinoma group (HCC), CHB, liver cirrhosis and hepatocellular carcinoma constitute HLD. We conducted three case-control studies: NeC (840 cases) vs. HLD (1792 cases), SC (486 cases) vs. HLD (1792 cases) and CHB + LC (1,371 cases) vs. HCC (421 cases). 11 polymorphic loci in MLH1, MLH3, MSH5, PMS1 and PMS2 were involved in genotyping by Sequenom MassArray. The SNPStats performed Hardy-Weinberg equilibrium test. Linkage disequilibrium patterns were visualized using Haploview4.2. The GMDR (v0.9) was conducted to generalized multifactor dimension reduction analysis. The correlation, multiplicative interaction and additive interaction analyses were calculated by Logistic Regression through SPSS21.0. Matrix and programmed excel were also involved in the calculation of additive interaction. Results: In NeC vs. HLD group, MSH5-rs1150793(G) was a risk base to HBV susceptibility (nominal p = 0.002, OR = 1.346). We found multiplicative interaction between MLH1-rs1540354 (AA + AT) and PMS1-rs1233255 (AA) (nominal p = 0.024, OR = 1.240). There was additive interaction between PMS1-rs1233255 (AA) and PMS1-rs256554(CA + CC). In SC vs. HLD group, MLH1-rs1540354 (TT) was a risk genotype (nominal p < 0.05, OR>1). Through haplotype analysis, we found the linkage disequilibrium of three loci in MLH1. The results of GMDR showed the optimal five-locus model about the spontaneous clearance of HBV. In CHB + LC vs. HCC group, PMS2-rs12112229(A) was related to the cancerization of liver. Conclusion: We found rs1150793(G), rs1540354(T) and rs12112229(A) were significantly related to HBV susceptibility, spontaneous clearance of HBV and cancerization after infection, respectively. Frontiers Media S.A. 2023-02-01 /pmc/articles/PMC9928949/ /pubmed/36816025 http://dx.doi.org/10.3389/fgene.2023.1077297 Text en Copyright © 2023 Ma, Sun, Kong, Jin, Yu, Liu, Yang, Liu, Gao, Liu, Zhang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Ma, Ning
Sun, Yucheng
Kong, Yanan
Jin, Yiyao
Yu, Fengxue
Liu, Lianfeng
Yang, Lei
Liu, Wenxuan
Gao, Xia
Liu, Dianwu
Zhang, Xiaolin
Li, Lu
Comprehensive investigating of mismatch repair genes (MMR) polymorphisms in participants with chronic hepatitis B virus infection
title Comprehensive investigating of mismatch repair genes (MMR) polymorphisms in participants with chronic hepatitis B virus infection
title_full Comprehensive investigating of mismatch repair genes (MMR) polymorphisms in participants with chronic hepatitis B virus infection
title_fullStr Comprehensive investigating of mismatch repair genes (MMR) polymorphisms in participants with chronic hepatitis B virus infection
title_full_unstemmed Comprehensive investigating of mismatch repair genes (MMR) polymorphisms in participants with chronic hepatitis B virus infection
title_short Comprehensive investigating of mismatch repair genes (MMR) polymorphisms in participants with chronic hepatitis B virus infection
title_sort comprehensive investigating of mismatch repair genes (mmr) polymorphisms in participants with chronic hepatitis b virus infection
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9928949/
https://www.ncbi.nlm.nih.gov/pubmed/36816025
http://dx.doi.org/10.3389/fgene.2023.1077297
work_keys_str_mv AT maning comprehensiveinvestigatingofmismatchrepairgenesmmrpolymorphismsinparticipantswithchronichepatitisbvirusinfection
AT sunyucheng comprehensiveinvestigatingofmismatchrepairgenesmmrpolymorphismsinparticipantswithchronichepatitisbvirusinfection
AT kongyanan comprehensiveinvestigatingofmismatchrepairgenesmmrpolymorphismsinparticipantswithchronichepatitisbvirusinfection
AT jinyiyao comprehensiveinvestigatingofmismatchrepairgenesmmrpolymorphismsinparticipantswithchronichepatitisbvirusinfection
AT yufengxue comprehensiveinvestigatingofmismatchrepairgenesmmrpolymorphismsinparticipantswithchronichepatitisbvirusinfection
AT liulianfeng comprehensiveinvestigatingofmismatchrepairgenesmmrpolymorphismsinparticipantswithchronichepatitisbvirusinfection
AT yanglei comprehensiveinvestigatingofmismatchrepairgenesmmrpolymorphismsinparticipantswithchronichepatitisbvirusinfection
AT liuwenxuan comprehensiveinvestigatingofmismatchrepairgenesmmrpolymorphismsinparticipantswithchronichepatitisbvirusinfection
AT gaoxia comprehensiveinvestigatingofmismatchrepairgenesmmrpolymorphismsinparticipantswithchronichepatitisbvirusinfection
AT liudianwu comprehensiveinvestigatingofmismatchrepairgenesmmrpolymorphismsinparticipantswithchronichepatitisbvirusinfection
AT zhangxiaolin comprehensiveinvestigatingofmismatchrepairgenesmmrpolymorphismsinparticipantswithchronichepatitisbvirusinfection
AT lilu comprehensiveinvestigatingofmismatchrepairgenesmmrpolymorphismsinparticipantswithchronichepatitisbvirusinfection