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Management of take-all disease caused by Gaeumannomyces graminis var. tritici in wheat through Bacillus subtilis strains

Wheat (Triticum aestivum) is the second largest grain crop worldwide, and one of the three major grain crops produced in China. Take-all disease, caused by Gaeumannomyces graminis var. tritici (Ggt) infection, is a widespread and devastating soil-borne disease that harms wheat production. At present...

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Autores principales: Zhao, Gangyi, Sun, Tianjie, Zhang, Zina, Zhang, Jingjing, Bian, Yinbo, Hou, Chunyan, Zhang, Dongdong, Han, Shengfang, Wang, Dongmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929034/
https://www.ncbi.nlm.nih.gov/pubmed/36819043
http://dx.doi.org/10.3389/fmicb.2023.1118176
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author Zhao, Gangyi
Sun, Tianjie
Zhang, Zina
Zhang, Jingjing
Bian, Yinbo
Hou, Chunyan
Zhang, Dongdong
Han, Shengfang
Wang, Dongmei
author_facet Zhao, Gangyi
Sun, Tianjie
Zhang, Zina
Zhang, Jingjing
Bian, Yinbo
Hou, Chunyan
Zhang, Dongdong
Han, Shengfang
Wang, Dongmei
author_sort Zhao, Gangyi
collection PubMed
description Wheat (Triticum aestivum) is the second largest grain crop worldwide, and one of the three major grain crops produced in China. Take-all disease, caused by Gaeumannomyces graminis var. tritici (Ggt) infection, is a widespread and devastating soil-borne disease that harms wheat production. At present, the prevention and control of wheat take-all depend largely on the application of chemical pesticides. Chemical pesticides, however, not only lead to increased drug resistance of pathogens but also leave significant residues in the soil, causing serious environmental pollution. In this study, we investigated the application of Bacillus subtilis to achieve take-all disease control in wheat while reducing pesticide application. Antagonistic bacteria were screened by plate test, species identification of strains was performed by Gram staining and sequencing of 16s rDNA, secondary metabolite activity of strains was detected by clear circle method, strain compatibility and effect of compounding on Ggt were detected by plate, and the application prospects of specific strains were analyzed by greenhouse and field experiments. We found that five B. subtilis strains, JY122, JY214, ZY133, NW03, Z-14, had significant antagonistic effects against Ggt, and could secrete antimicrobial proteins including amylase, protease, and cellulase. Furthermore, Z-14 and JY214 cultures have also been shown to change the morphology of Ggt mycelium. These results also showed that Z-14, JY214, and their combination can control take-all disease in wheat at a reduced level of pesticide use. In summary, we screened two Bacillus spp. strains, Z-14 and JY214, that could act as antagonists that contribute to the biological control of wheat take-all disease. These findings provide resources and ideas for controlling crop diseases in an environmentally friendly manner.
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spelling pubmed-99290342023-02-16 Management of take-all disease caused by Gaeumannomyces graminis var. tritici in wheat through Bacillus subtilis strains Zhao, Gangyi Sun, Tianjie Zhang, Zina Zhang, Jingjing Bian, Yinbo Hou, Chunyan Zhang, Dongdong Han, Shengfang Wang, Dongmei Front Microbiol Microbiology Wheat (Triticum aestivum) is the second largest grain crop worldwide, and one of the three major grain crops produced in China. Take-all disease, caused by Gaeumannomyces graminis var. tritici (Ggt) infection, is a widespread and devastating soil-borne disease that harms wheat production. At present, the prevention and control of wheat take-all depend largely on the application of chemical pesticides. Chemical pesticides, however, not only lead to increased drug resistance of pathogens but also leave significant residues in the soil, causing serious environmental pollution. In this study, we investigated the application of Bacillus subtilis to achieve take-all disease control in wheat while reducing pesticide application. Antagonistic bacteria were screened by plate test, species identification of strains was performed by Gram staining and sequencing of 16s rDNA, secondary metabolite activity of strains was detected by clear circle method, strain compatibility and effect of compounding on Ggt were detected by plate, and the application prospects of specific strains were analyzed by greenhouse and field experiments. We found that five B. subtilis strains, JY122, JY214, ZY133, NW03, Z-14, had significant antagonistic effects against Ggt, and could secrete antimicrobial proteins including amylase, protease, and cellulase. Furthermore, Z-14 and JY214 cultures have also been shown to change the morphology of Ggt mycelium. These results also showed that Z-14, JY214, and their combination can control take-all disease in wheat at a reduced level of pesticide use. In summary, we screened two Bacillus spp. strains, Z-14 and JY214, that could act as antagonists that contribute to the biological control of wheat take-all disease. These findings provide resources and ideas for controlling crop diseases in an environmentally friendly manner. Frontiers Media S.A. 2023-02-01 /pmc/articles/PMC9929034/ /pubmed/36819043 http://dx.doi.org/10.3389/fmicb.2023.1118176 Text en Copyright © 2023 Zhao, Sun, Zhang, Zhang, Bian, Hou, Zhang, Han and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhao, Gangyi
Sun, Tianjie
Zhang, Zina
Zhang, Jingjing
Bian, Yinbo
Hou, Chunyan
Zhang, Dongdong
Han, Shengfang
Wang, Dongmei
Management of take-all disease caused by Gaeumannomyces graminis var. tritici in wheat through Bacillus subtilis strains
title Management of take-all disease caused by Gaeumannomyces graminis var. tritici in wheat through Bacillus subtilis strains
title_full Management of take-all disease caused by Gaeumannomyces graminis var. tritici in wheat through Bacillus subtilis strains
title_fullStr Management of take-all disease caused by Gaeumannomyces graminis var. tritici in wheat through Bacillus subtilis strains
title_full_unstemmed Management of take-all disease caused by Gaeumannomyces graminis var. tritici in wheat through Bacillus subtilis strains
title_short Management of take-all disease caused by Gaeumannomyces graminis var. tritici in wheat through Bacillus subtilis strains
title_sort management of take-all disease caused by gaeumannomyces graminis var. tritici in wheat through bacillus subtilis strains
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929034/
https://www.ncbi.nlm.nih.gov/pubmed/36819043
http://dx.doi.org/10.3389/fmicb.2023.1118176
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