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Prognostic and immunological role of sulfatide-related lncRNAs in hepatocellular carcinoma
BACKGROUND: Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver. Long non-coding RNAs (lncRNAs) play important roles in the occurrence and development of HCC through multiple pathways. Our previous study reported the specific molecular mechanism for sulfatide regulation...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929346/ https://www.ncbi.nlm.nih.gov/pubmed/36816914 http://dx.doi.org/10.3389/fonc.2023.1091132 |
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author | Huang, Xing Feng Fu, Li Sheng Cai, Qian Qian Fan, Fei |
author_facet | Huang, Xing Feng Fu, Li Sheng Cai, Qian Qian Fan, Fei |
author_sort | Huang, Xing Feng |
collection | PubMed |
description | BACKGROUND: Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver. Long non-coding RNAs (lncRNAs) play important roles in the occurrence and development of HCC through multiple pathways. Our previous study reported the specific molecular mechanism for sulfatide regulation of integrin αV expression and cell adhesion in HCC cells through lncRNA AY927503. Next, it is necessary to identify more sulfatide-related lncRNAs, explore their clinical signifcance, and determine new targeted treatment strategies. METHODS: Microarrays were used to screen a complete set of lncRNAs with different expression profiles in sulfatide-treated cells. Sulfatide-related lncRNAs expression data and corresponding HCC patient survival information were obtained from the The Cancer Genome Atlas (TCGA) database, and the prognosis prediction model was constructed based on Cox regression analysis. Methylated RNA immunoprecipitation with next generation sequencing (MeRIP-seq) was used to detemine the effect of sulfatide on lncRNAs m6A modification. Tumor Immune Estimation Resource (TIMER) and Gene set nnrichment analysis (GSEA) were utilized to enrich the immune and functional pathways of sulfatide-related lncRNAs. RESULTS: A total of 85 differentially expressed lncRNAs (|Fold Change (FC)|>2, P<0.05) were screened in sulfatide-treated HCC cells. As a result, 24 sulfatide-related lncRNAs were highly expressed in HCC tissues, six of which were associated with poor prognosis in HCC patients. Based on thses data, a sulfatide-related lncRNAs prognosis assessment model for HCC was constructed. According to this risk score analysis, the overall survival (OS) curve showed that the OS of high-risk patients was significantly lower than that of low-risk patients (P<0.05). Notably, the expression difference in sulfatide-related lncRNA NRSN2-AS1 may be related to sulfatide-induced RNA m6A methylation. In addition, the expression level of NRSN2-AS1 was significantly positively correlated with immune cell infiltration in HCC and participated in the peroxisome and Peroxisome proliferator-activated receptor (PPAR) signaling pathways. CONCLUSIONS: In conclusion, sulfatide-related lncRNAs might be promising prognostic and therapeutic targets for HCC. |
format | Online Article Text |
id | pubmed-9929346 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99293462023-02-16 Prognostic and immunological role of sulfatide-related lncRNAs in hepatocellular carcinoma Huang, Xing Feng Fu, Li Sheng Cai, Qian Qian Fan, Fei Front Oncol Oncology BACKGROUND: Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver. Long non-coding RNAs (lncRNAs) play important roles in the occurrence and development of HCC through multiple pathways. Our previous study reported the specific molecular mechanism for sulfatide regulation of integrin αV expression and cell adhesion in HCC cells through lncRNA AY927503. Next, it is necessary to identify more sulfatide-related lncRNAs, explore their clinical signifcance, and determine new targeted treatment strategies. METHODS: Microarrays were used to screen a complete set of lncRNAs with different expression profiles in sulfatide-treated cells. Sulfatide-related lncRNAs expression data and corresponding HCC patient survival information were obtained from the The Cancer Genome Atlas (TCGA) database, and the prognosis prediction model was constructed based on Cox regression analysis. Methylated RNA immunoprecipitation with next generation sequencing (MeRIP-seq) was used to detemine the effect of sulfatide on lncRNAs m6A modification. Tumor Immune Estimation Resource (TIMER) and Gene set nnrichment analysis (GSEA) were utilized to enrich the immune and functional pathways of sulfatide-related lncRNAs. RESULTS: A total of 85 differentially expressed lncRNAs (|Fold Change (FC)|>2, P<0.05) were screened in sulfatide-treated HCC cells. As a result, 24 sulfatide-related lncRNAs were highly expressed in HCC tissues, six of which were associated with poor prognosis in HCC patients. Based on thses data, a sulfatide-related lncRNAs prognosis assessment model for HCC was constructed. According to this risk score analysis, the overall survival (OS) curve showed that the OS of high-risk patients was significantly lower than that of low-risk patients (P<0.05). Notably, the expression difference in sulfatide-related lncRNA NRSN2-AS1 may be related to sulfatide-induced RNA m6A methylation. In addition, the expression level of NRSN2-AS1 was significantly positively correlated with immune cell infiltration in HCC and participated in the peroxisome and Peroxisome proliferator-activated receptor (PPAR) signaling pathways. CONCLUSIONS: In conclusion, sulfatide-related lncRNAs might be promising prognostic and therapeutic targets for HCC. Frontiers Media S.A. 2023-02-01 /pmc/articles/PMC9929346/ /pubmed/36816914 http://dx.doi.org/10.3389/fonc.2023.1091132 Text en Copyright © 2023 Huang, Fu, Cai and Fan https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Huang, Xing Feng Fu, Li Sheng Cai, Qian Qian Fan, Fei Prognostic and immunological role of sulfatide-related lncRNAs in hepatocellular carcinoma |
title | Prognostic and immunological role of sulfatide-related lncRNAs in hepatocellular carcinoma |
title_full | Prognostic and immunological role of sulfatide-related lncRNAs in hepatocellular carcinoma |
title_fullStr | Prognostic and immunological role of sulfatide-related lncRNAs in hepatocellular carcinoma |
title_full_unstemmed | Prognostic and immunological role of sulfatide-related lncRNAs in hepatocellular carcinoma |
title_short | Prognostic and immunological role of sulfatide-related lncRNAs in hepatocellular carcinoma |
title_sort | prognostic and immunological role of sulfatide-related lncrnas in hepatocellular carcinoma |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929346/ https://www.ncbi.nlm.nih.gov/pubmed/36816914 http://dx.doi.org/10.3389/fonc.2023.1091132 |
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