Mechanical conditioning of human mesenchymal stem cells for enhancing vascular regeneration

Human mesenchymal stem cells (hMSCs) are an appealing cell type for therapeutic applications but remain limited by poor efficacy in clinical trials. Here, we describe a conditioning technique that enhances the vascular regenerative properties of hMSCs and increases their expression of endothelial ce...

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Detalles Bibliográficos
Autores principales: Massidda, Miles W., Im, ByungGee, Lee, Jason, Baker, Aaron B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929623/
https://www.ncbi.nlm.nih.gov/pubmed/36853695
http://dx.doi.org/10.1016/j.xpro.2023.102103
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author Massidda, Miles W.
Im, ByungGee
Lee, Jason
Baker, Aaron B.
author_facet Massidda, Miles W.
Im, ByungGee
Lee, Jason
Baker, Aaron B.
author_sort Massidda, Miles W.
collection PubMed
description Human mesenchymal stem cells (hMSCs) are an appealing cell type for therapeutic applications but remain limited by poor efficacy in clinical trials. Here, we describe a conditioning technique that enhances the vascular regenerative properties of hMSCs and increases their expression of endothelial cell and pericyte markers. We also describe an alginate gel encapsulation protocol for delivering the conditioned cells. For complete details on the use and execution of this protocol, please refer to Lee et al. (2021).(1)
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spelling pubmed-99296232023-02-16 Mechanical conditioning of human mesenchymal stem cells for enhancing vascular regeneration Massidda, Miles W. Im, ByungGee Lee, Jason Baker, Aaron B. STAR Protoc Protocol Human mesenchymal stem cells (hMSCs) are an appealing cell type for therapeutic applications but remain limited by poor efficacy in clinical trials. Here, we describe a conditioning technique that enhances the vascular regenerative properties of hMSCs and increases their expression of endothelial cell and pericyte markers. We also describe an alginate gel encapsulation protocol for delivering the conditioned cells. For complete details on the use and execution of this protocol, please refer to Lee et al. (2021).(1) Elsevier 2023-02-07 /pmc/articles/PMC9929623/ /pubmed/36853695 http://dx.doi.org/10.1016/j.xpro.2023.102103 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Massidda, Miles W.
Im, ByungGee
Lee, Jason
Baker, Aaron B.
Mechanical conditioning of human mesenchymal stem cells for enhancing vascular regeneration
title Mechanical conditioning of human mesenchymal stem cells for enhancing vascular regeneration
title_full Mechanical conditioning of human mesenchymal stem cells for enhancing vascular regeneration
title_fullStr Mechanical conditioning of human mesenchymal stem cells for enhancing vascular regeneration
title_full_unstemmed Mechanical conditioning of human mesenchymal stem cells for enhancing vascular regeneration
title_short Mechanical conditioning of human mesenchymal stem cells for enhancing vascular regeneration
title_sort mechanical conditioning of human mesenchymal stem cells for enhancing vascular regeneration
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929623/
https://www.ncbi.nlm.nih.gov/pubmed/36853695
http://dx.doi.org/10.1016/j.xpro.2023.102103
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