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Proteomics comparison between mass-like and non-mass-like breast lesions

BACKGROUND: The proteomic differences between mass-like (ML) breast lesions and non-mass-like (NML) breast lesions were compared to explore the formation mechanism of NML ultrasonic morphological characteristics. METHODS: From January to August 2021, tissue samples were collected from 10 patients wi...

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Autores principales: Li, Shi-Yu, Wang, Bo, Jiang, Ying, Li, Junkang, Liu, Gang, Wang, Zhi-Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929763/
https://www.ncbi.nlm.nih.gov/pubmed/36819556
http://dx.doi.org/10.21037/atm-22-6655
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author Li, Shi-Yu
Wang, Bo
Jiang, Ying
Li, Junkang
Liu, Gang
Wang, Zhi-Li
author_facet Li, Shi-Yu
Wang, Bo
Jiang, Ying
Li, Junkang
Liu, Gang
Wang, Zhi-Li
author_sort Li, Shi-Yu
collection PubMed
description BACKGROUND: The proteomic differences between mass-like (ML) breast lesions and non-mass-like (NML) breast lesions were compared to explore the formation mechanism of NML ultrasonic morphological characteristics. METHODS: From January to August 2021, tissue samples were collected from 10 patients with malignant ML (MML), 10 patients with malignant NML (MNML), 10 patients with benign ML (BML), and seven patients with benign NML (BNML). The proteomic differences between the BML and BNML groups and the MML and MNML groups were compared by data-independent acquisition (DIA) quantitative mass spectrometry. The differentially expressed proteins were analyzed by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI) networks analyses. RESULTS: We identified a total of 623 significantly differentially expressed proteins in the MML/MNML group, and 167 significantly differentially expressed proteins in the BML/BNML group, with relative ratios >1.2 or <−0.83. The up-regulated differential proteins were more abundant in the tumor necrosis factor (TNF) signaling pathway in both the MML/MNML and BML/BNML groups, suggesting that the TNF signaling pathway may be related to the ultrasonic morphological characteristics of breast lesions. Dual specificity mitogen-activated protein kinase 3 (MP2K3), a protein factor in the TNF signaling pathway, exhibited significant upregulation in both the malignant and BML groups. CONCLUSIONS: The TNF signaling pathway may be associated with the ultrasonic morphological characteristics of breast lesions. MP2K3 is the up-regulated differential expression protein in the MML/MNML and BML/BNML groups, which may be related to the ultrasonic morphological characteristics of breast lesions.
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spelling pubmed-99297632023-02-16 Proteomics comparison between mass-like and non-mass-like breast lesions Li, Shi-Yu Wang, Bo Jiang, Ying Li, Junkang Liu, Gang Wang, Zhi-Li Ann Transl Med Original Article BACKGROUND: The proteomic differences between mass-like (ML) breast lesions and non-mass-like (NML) breast lesions were compared to explore the formation mechanism of NML ultrasonic morphological characteristics. METHODS: From January to August 2021, tissue samples were collected from 10 patients with malignant ML (MML), 10 patients with malignant NML (MNML), 10 patients with benign ML (BML), and seven patients with benign NML (BNML). The proteomic differences between the BML and BNML groups and the MML and MNML groups were compared by data-independent acquisition (DIA) quantitative mass spectrometry. The differentially expressed proteins were analyzed by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI) networks analyses. RESULTS: We identified a total of 623 significantly differentially expressed proteins in the MML/MNML group, and 167 significantly differentially expressed proteins in the BML/BNML group, with relative ratios >1.2 or <−0.83. The up-regulated differential proteins were more abundant in the tumor necrosis factor (TNF) signaling pathway in both the MML/MNML and BML/BNML groups, suggesting that the TNF signaling pathway may be related to the ultrasonic morphological characteristics of breast lesions. Dual specificity mitogen-activated protein kinase 3 (MP2K3), a protein factor in the TNF signaling pathway, exhibited significant upregulation in both the malignant and BML groups. CONCLUSIONS: The TNF signaling pathway may be associated with the ultrasonic morphological characteristics of breast lesions. MP2K3 is the up-regulated differential expression protein in the MML/MNML and BML/BNML groups, which may be related to the ultrasonic morphological characteristics of breast lesions. AME Publishing Company 2023-01-31 2023-01-31 /pmc/articles/PMC9929763/ /pubmed/36819556 http://dx.doi.org/10.21037/atm-22-6655 Text en 2023 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Li, Shi-Yu
Wang, Bo
Jiang, Ying
Li, Junkang
Liu, Gang
Wang, Zhi-Li
Proteomics comparison between mass-like and non-mass-like breast lesions
title Proteomics comparison between mass-like and non-mass-like breast lesions
title_full Proteomics comparison between mass-like and non-mass-like breast lesions
title_fullStr Proteomics comparison between mass-like and non-mass-like breast lesions
title_full_unstemmed Proteomics comparison between mass-like and non-mass-like breast lesions
title_short Proteomics comparison between mass-like and non-mass-like breast lesions
title_sort proteomics comparison between mass-like and non-mass-like breast lesions
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929763/
https://www.ncbi.nlm.nih.gov/pubmed/36819556
http://dx.doi.org/10.21037/atm-22-6655
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