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Proteomics comparison between mass-like and non-mass-like breast lesions
BACKGROUND: The proteomic differences between mass-like (ML) breast lesions and non-mass-like (NML) breast lesions were compared to explore the formation mechanism of NML ultrasonic morphological characteristics. METHODS: From January to August 2021, tissue samples were collected from 10 patients wi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AME Publishing Company
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929763/ https://www.ncbi.nlm.nih.gov/pubmed/36819556 http://dx.doi.org/10.21037/atm-22-6655 |
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author | Li, Shi-Yu Wang, Bo Jiang, Ying Li, Junkang Liu, Gang Wang, Zhi-Li |
author_facet | Li, Shi-Yu Wang, Bo Jiang, Ying Li, Junkang Liu, Gang Wang, Zhi-Li |
author_sort | Li, Shi-Yu |
collection | PubMed |
description | BACKGROUND: The proteomic differences between mass-like (ML) breast lesions and non-mass-like (NML) breast lesions were compared to explore the formation mechanism of NML ultrasonic morphological characteristics. METHODS: From January to August 2021, tissue samples were collected from 10 patients with malignant ML (MML), 10 patients with malignant NML (MNML), 10 patients with benign ML (BML), and seven patients with benign NML (BNML). The proteomic differences between the BML and BNML groups and the MML and MNML groups were compared by data-independent acquisition (DIA) quantitative mass spectrometry. The differentially expressed proteins were analyzed by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI) networks analyses. RESULTS: We identified a total of 623 significantly differentially expressed proteins in the MML/MNML group, and 167 significantly differentially expressed proteins in the BML/BNML group, with relative ratios >1.2 or <−0.83. The up-regulated differential proteins were more abundant in the tumor necrosis factor (TNF) signaling pathway in both the MML/MNML and BML/BNML groups, suggesting that the TNF signaling pathway may be related to the ultrasonic morphological characteristics of breast lesions. Dual specificity mitogen-activated protein kinase 3 (MP2K3), a protein factor in the TNF signaling pathway, exhibited significant upregulation in both the malignant and BML groups. CONCLUSIONS: The TNF signaling pathway may be associated with the ultrasonic morphological characteristics of breast lesions. MP2K3 is the up-regulated differential expression protein in the MML/MNML and BML/BNML groups, which may be related to the ultrasonic morphological characteristics of breast lesions. |
format | Online Article Text |
id | pubmed-9929763 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | AME Publishing Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-99297632023-02-16 Proteomics comparison between mass-like and non-mass-like breast lesions Li, Shi-Yu Wang, Bo Jiang, Ying Li, Junkang Liu, Gang Wang, Zhi-Li Ann Transl Med Original Article BACKGROUND: The proteomic differences between mass-like (ML) breast lesions and non-mass-like (NML) breast lesions were compared to explore the formation mechanism of NML ultrasonic morphological characteristics. METHODS: From January to August 2021, tissue samples were collected from 10 patients with malignant ML (MML), 10 patients with malignant NML (MNML), 10 patients with benign ML (BML), and seven patients with benign NML (BNML). The proteomic differences between the BML and BNML groups and the MML and MNML groups were compared by data-independent acquisition (DIA) quantitative mass spectrometry. The differentially expressed proteins were analyzed by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI) networks analyses. RESULTS: We identified a total of 623 significantly differentially expressed proteins in the MML/MNML group, and 167 significantly differentially expressed proteins in the BML/BNML group, with relative ratios >1.2 or <−0.83. The up-regulated differential proteins were more abundant in the tumor necrosis factor (TNF) signaling pathway in both the MML/MNML and BML/BNML groups, suggesting that the TNF signaling pathway may be related to the ultrasonic morphological characteristics of breast lesions. Dual specificity mitogen-activated protein kinase 3 (MP2K3), a protein factor in the TNF signaling pathway, exhibited significant upregulation in both the malignant and BML groups. CONCLUSIONS: The TNF signaling pathway may be associated with the ultrasonic morphological characteristics of breast lesions. MP2K3 is the up-regulated differential expression protein in the MML/MNML and BML/BNML groups, which may be related to the ultrasonic morphological characteristics of breast lesions. AME Publishing Company 2023-01-31 2023-01-31 /pmc/articles/PMC9929763/ /pubmed/36819556 http://dx.doi.org/10.21037/atm-22-6655 Text en 2023 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Original Article Li, Shi-Yu Wang, Bo Jiang, Ying Li, Junkang Liu, Gang Wang, Zhi-Li Proteomics comparison between mass-like and non-mass-like breast lesions |
title | Proteomics comparison between mass-like and non-mass-like breast lesions |
title_full | Proteomics comparison between mass-like and non-mass-like breast lesions |
title_fullStr | Proteomics comparison between mass-like and non-mass-like breast lesions |
title_full_unstemmed | Proteomics comparison between mass-like and non-mass-like breast lesions |
title_short | Proteomics comparison between mass-like and non-mass-like breast lesions |
title_sort | proteomics comparison between mass-like and non-mass-like breast lesions |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929763/ https://www.ncbi.nlm.nih.gov/pubmed/36819556 http://dx.doi.org/10.21037/atm-22-6655 |
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