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Rapid molecular imaging of active thrombi in vivo using aptamer-antidote probes
Pathological blood clotting, or thrombosis, limits vital blood flow to organs; such deprivation can lead to catastrophic events including myocardial infarction, pulmonary embolism, and ischemic stroke. Prompt restoration of blood flow greatly improves outcomes. We explored whether aptamers could ser...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Gene & Cell Therapy
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930157/ https://www.ncbi.nlm.nih.gov/pubmed/36817726 http://dx.doi.org/10.1016/j.omtn.2023.01.009 |
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author | Gray, Bethany Powell Kelly, Linsley Steen-Burrell, Kady-Ann Layzer, Juliana M. Rempel, Rachel E. Nimjee, Shahid M. Cooley, Brian C. Tarantal, Alice F. Sullenger, Bruce A. |
author_facet | Gray, Bethany Powell Kelly, Linsley Steen-Burrell, Kady-Ann Layzer, Juliana M. Rempel, Rachel E. Nimjee, Shahid M. Cooley, Brian C. Tarantal, Alice F. Sullenger, Bruce A. |
author_sort | Gray, Bethany Powell |
collection | PubMed |
description | Pathological blood clotting, or thrombosis, limits vital blood flow to organs; such deprivation can lead to catastrophic events including myocardial infarction, pulmonary embolism, and ischemic stroke. Prompt restoration of blood flow greatly improves outcomes. We explored whether aptamers could serve as molecular imaging probes to rapidly detect thrombi. An aptamer targeting thrombin, Tog25t, was found to rapidly localize to and visualize pre-existing clots in the femoral and jugular veins of mice using fluorescence imaging and, when circulating, was able to image clots as they form. Since free aptamer is quickly cleared from circulation, contrast is rapidly developed, allowing clot visualization within minutes. Moreover, administration of an antidote oligonucleotide further enhanced contrast development, causing the unbound aptamer to clear within 5min while impacting the clot-bound aptamer more slowly. These findings suggest that aptamers can serve as imaging agents for rapid detection of thrombi in acute care and perioperative settings. |
format | Online Article Text |
id | pubmed-9930157 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Society of Gene & Cell Therapy |
record_format | MEDLINE/PubMed |
spelling | pubmed-99301572023-02-16 Rapid molecular imaging of active thrombi in vivo using aptamer-antidote probes Gray, Bethany Powell Kelly, Linsley Steen-Burrell, Kady-Ann Layzer, Juliana M. Rempel, Rachel E. Nimjee, Shahid M. Cooley, Brian C. Tarantal, Alice F. Sullenger, Bruce A. Mol Ther Nucleic Acids Original Article Pathological blood clotting, or thrombosis, limits vital blood flow to organs; such deprivation can lead to catastrophic events including myocardial infarction, pulmonary embolism, and ischemic stroke. Prompt restoration of blood flow greatly improves outcomes. We explored whether aptamers could serve as molecular imaging probes to rapidly detect thrombi. An aptamer targeting thrombin, Tog25t, was found to rapidly localize to and visualize pre-existing clots in the femoral and jugular veins of mice using fluorescence imaging and, when circulating, was able to image clots as they form. Since free aptamer is quickly cleared from circulation, contrast is rapidly developed, allowing clot visualization within minutes. Moreover, administration of an antidote oligonucleotide further enhanced contrast development, causing the unbound aptamer to clear within 5min while impacting the clot-bound aptamer more slowly. These findings suggest that aptamers can serve as imaging agents for rapid detection of thrombi in acute care and perioperative settings. American Society of Gene & Cell Therapy 2023-01-21 /pmc/articles/PMC9930157/ /pubmed/36817726 http://dx.doi.org/10.1016/j.omtn.2023.01.009 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Gray, Bethany Powell Kelly, Linsley Steen-Burrell, Kady-Ann Layzer, Juliana M. Rempel, Rachel E. Nimjee, Shahid M. Cooley, Brian C. Tarantal, Alice F. Sullenger, Bruce A. Rapid molecular imaging of active thrombi in vivo using aptamer-antidote probes |
title | Rapid molecular imaging of active thrombi in vivo using aptamer-antidote probes |
title_full | Rapid molecular imaging of active thrombi in vivo using aptamer-antidote probes |
title_fullStr | Rapid molecular imaging of active thrombi in vivo using aptamer-antidote probes |
title_full_unstemmed | Rapid molecular imaging of active thrombi in vivo using aptamer-antidote probes |
title_short | Rapid molecular imaging of active thrombi in vivo using aptamer-antidote probes |
title_sort | rapid molecular imaging of active thrombi in vivo using aptamer-antidote probes |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930157/ https://www.ncbi.nlm.nih.gov/pubmed/36817726 http://dx.doi.org/10.1016/j.omtn.2023.01.009 |
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