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SWR1 chromatin remodeling complex prevents mitotic slippage during spindle position checkpoint arrest
Faithful chromosome segregation in budding yeast requires correct positioning of the mitotic spindle along the mother to daughter cell polarity axis. When the anaphase spindle is not correctly positioned, a surveillance mechanism, named as the spindle position checkpoint (SPOC), prevents the progres...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930528/ https://www.ncbi.nlm.nih.gov/pubmed/36542480 http://dx.doi.org/10.1091/mbc.E20-03-0179 |
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author | Caydasi, Ayse Koca Khmelinskii, Anton Darieva, Zoulfia Kurtulmus, Bahtiyar Knop, Michael Pereira, Gislene |
author_facet | Caydasi, Ayse Koca Khmelinskii, Anton Darieva, Zoulfia Kurtulmus, Bahtiyar Knop, Michael Pereira, Gislene |
author_sort | Caydasi, Ayse Koca |
collection | PubMed |
description | Faithful chromosome segregation in budding yeast requires correct positioning of the mitotic spindle along the mother to daughter cell polarity axis. When the anaphase spindle is not correctly positioned, a surveillance mechanism, named as the spindle position checkpoint (SPOC), prevents the progression out of mitosis until correct spindle positioning is achieved. How SPOC works on a molecular level is not well understood. Here we performed a genome-wide genetic screen to search for components required for SPOC. We identified the SWR1 chromatin-remodeling complex (SWR1-C) among several novel factors that are essential for SPOC integrity. Cells lacking SWR1-C were able to activate SPOC upon spindle misorientation but underwent mitotic slippage upon prolonged SPOC arrest. This mitotic slippage required the Cdc14-early anaphase release pathway and other factors including the SAGA (Spt-Ada-Gcn5 acetyltransferase) histone acetyltransferase complex, proteasome components and the mitotic cyclin-dependent kinase inhibitor Sic1. Together, our data establish a novel link between SWR1-C chromatin remodeling and robust checkpoint arrest in late anaphase. |
format | Online Article Text |
id | pubmed-9930528 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-99305282023-03-28 SWR1 chromatin remodeling complex prevents mitotic slippage during spindle position checkpoint arrest Caydasi, Ayse Koca Khmelinskii, Anton Darieva, Zoulfia Kurtulmus, Bahtiyar Knop, Michael Pereira, Gislene Mol Biol Cell Articles Faithful chromosome segregation in budding yeast requires correct positioning of the mitotic spindle along the mother to daughter cell polarity axis. When the anaphase spindle is not correctly positioned, a surveillance mechanism, named as the spindle position checkpoint (SPOC), prevents the progression out of mitosis until correct spindle positioning is achieved. How SPOC works on a molecular level is not well understood. Here we performed a genome-wide genetic screen to search for components required for SPOC. We identified the SWR1 chromatin-remodeling complex (SWR1-C) among several novel factors that are essential for SPOC integrity. Cells lacking SWR1-C were able to activate SPOC upon spindle misorientation but underwent mitotic slippage upon prolonged SPOC arrest. This mitotic slippage required the Cdc14-early anaphase release pathway and other factors including the SAGA (Spt-Ada-Gcn5 acetyltransferase) histone acetyltransferase complex, proteasome components and the mitotic cyclin-dependent kinase inhibitor Sic1. Together, our data establish a novel link between SWR1-C chromatin remodeling and robust checkpoint arrest in late anaphase. The American Society for Cell Biology 2023-01-13 /pmc/articles/PMC9930528/ /pubmed/36542480 http://dx.doi.org/10.1091/mbc.E20-03-0179 Text en © 2023 Caydasi et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 4.0 Unported Creative Commons License. |
spellingShingle | Articles Caydasi, Ayse Koca Khmelinskii, Anton Darieva, Zoulfia Kurtulmus, Bahtiyar Knop, Michael Pereira, Gislene SWR1 chromatin remodeling complex prevents mitotic slippage during spindle position checkpoint arrest |
title | SWR1 chromatin remodeling complex prevents mitotic slippage during spindle position checkpoint arrest |
title_full | SWR1 chromatin remodeling complex prevents mitotic slippage during spindle position checkpoint arrest |
title_fullStr | SWR1 chromatin remodeling complex prevents mitotic slippage during spindle position checkpoint arrest |
title_full_unstemmed | SWR1 chromatin remodeling complex prevents mitotic slippage during spindle position checkpoint arrest |
title_short | SWR1 chromatin remodeling complex prevents mitotic slippage during spindle position checkpoint arrest |
title_sort | swr1 chromatin remodeling complex prevents mitotic slippage during spindle position checkpoint arrest |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930528/ https://www.ncbi.nlm.nih.gov/pubmed/36542480 http://dx.doi.org/10.1091/mbc.E20-03-0179 |
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