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Upregulation of p16(INK4A) in Peripheral White Blood Cells as a Novel Screening Marker for Colorectal Carcinoma

OBJECTIVE: Screening of colorectal cancer (CRC) is important for the early detection. CRC is relating to aging and immuno-senescence. One such senescent marker is p16(INK4A) expression in immune cells. The objective of the study is to investigate the protein expression of p16(INK4A) in peripheral wh...

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Autores principales: Thin, Khin Aye, Angsuwatcharakon, Phonthep, Edwards, Steven W, Mutirangura, Apiwat, Puttipanyalears, Charoenchai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930939/
https://www.ncbi.nlm.nih.gov/pubmed/36444588
http://dx.doi.org/10.31557/APJCP.2022.23.11.3753
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author Thin, Khin Aye
Angsuwatcharakon, Phonthep
Edwards, Steven W
Mutirangura, Apiwat
Puttipanyalears, Charoenchai
author_facet Thin, Khin Aye
Angsuwatcharakon, Phonthep
Edwards, Steven W
Mutirangura, Apiwat
Puttipanyalears, Charoenchai
author_sort Thin, Khin Aye
collection PubMed
description OBJECTIVE: Screening of colorectal cancer (CRC) is important for the early detection. CRC is relating to aging and immuno-senescence. One such senescent marker is p16(INK4A) expression in immune cells. The objective of the study is to investigate the protein expression of p16(INK4A) in peripheral white blood cells as a screening marker for colorectal cancer. METHODS: A case-control studies were conducted. Cases were patients with colorectal cancer and controls were matched with cases based on age and sex. Peripheral blood was collected from patients and controls and the protein p16(INK4A) was measured with immunofluorescent techniques. The p16(INK4A) levels from cases and controls were evaluated using ROC analysis to be used as a screening marker in CRC patients. Mean fluorescent intensity of p16(INK4A) of cases and controls were analyzed in CD45+, CD3+ or CD14+ cells. The p16(INK4A) levels of cases were also correlated with clinical data. RESULT: Statistically significant increased expression of p16(INK4A) levels were found in cases compared to controls. p16(INK4A) in peripheral immune cells had 78% sensitivity and 71% specificity which can possibly be used as a diagnosis tool for colorectal cancer. P16(INK4A)-positive cell percentage and mean florescent intensity were significantly higher in CD45+ cells, CD3 positive cells and CD14 positive cells. No significant correlation was observed with the clinical data and p16(INK4A) level of CRC patients. CONCLUSION: The significant increase of p16 (INK4A) expression level in peripheral immune cells represents potential for use as a CRC screening marker.
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spelling pubmed-99309392023-02-16 Upregulation of p16(INK4A) in Peripheral White Blood Cells as a Novel Screening Marker for Colorectal Carcinoma Thin, Khin Aye Angsuwatcharakon, Phonthep Edwards, Steven W Mutirangura, Apiwat Puttipanyalears, Charoenchai Asian Pac J Cancer Prev Research Article OBJECTIVE: Screening of colorectal cancer (CRC) is important for the early detection. CRC is relating to aging and immuno-senescence. One such senescent marker is p16(INK4A) expression in immune cells. The objective of the study is to investigate the protein expression of p16(INK4A) in peripheral white blood cells as a screening marker for colorectal cancer. METHODS: A case-control studies were conducted. Cases were patients with colorectal cancer and controls were matched with cases based on age and sex. Peripheral blood was collected from patients and controls and the protein p16(INK4A) was measured with immunofluorescent techniques. The p16(INK4A) levels from cases and controls were evaluated using ROC analysis to be used as a screening marker in CRC patients. Mean fluorescent intensity of p16(INK4A) of cases and controls were analyzed in CD45+, CD3+ or CD14+ cells. The p16(INK4A) levels of cases were also correlated with clinical data. RESULT: Statistically significant increased expression of p16(INK4A) levels were found in cases compared to controls. p16(INK4A) in peripheral immune cells had 78% sensitivity and 71% specificity which can possibly be used as a diagnosis tool for colorectal cancer. P16(INK4A)-positive cell percentage and mean florescent intensity were significantly higher in CD45+ cells, CD3 positive cells and CD14 positive cells. No significant correlation was observed with the clinical data and p16(INK4A) level of CRC patients. CONCLUSION: The significant increase of p16 (INK4A) expression level in peripheral immune cells represents potential for use as a CRC screening marker. West Asia Organization for Cancer Prevention 2022-11 /pmc/articles/PMC9930939/ /pubmed/36444588 http://dx.doi.org/10.31557/APJCP.2022.23.11.3753 Text en https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License. https://creativecommons.org/licenses/by-nc/4.0/
spellingShingle Research Article
Thin, Khin Aye
Angsuwatcharakon, Phonthep
Edwards, Steven W
Mutirangura, Apiwat
Puttipanyalears, Charoenchai
Upregulation of p16(INK4A) in Peripheral White Blood Cells as a Novel Screening Marker for Colorectal Carcinoma
title Upregulation of p16(INK4A) in Peripheral White Blood Cells as a Novel Screening Marker for Colorectal Carcinoma
title_full Upregulation of p16(INK4A) in Peripheral White Blood Cells as a Novel Screening Marker for Colorectal Carcinoma
title_fullStr Upregulation of p16(INK4A) in Peripheral White Blood Cells as a Novel Screening Marker for Colorectal Carcinoma
title_full_unstemmed Upregulation of p16(INK4A) in Peripheral White Blood Cells as a Novel Screening Marker for Colorectal Carcinoma
title_short Upregulation of p16(INK4A) in Peripheral White Blood Cells as a Novel Screening Marker for Colorectal Carcinoma
title_sort upregulation of p16(ink4a) in peripheral white blood cells as a novel screening marker for colorectal carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930939/
https://www.ncbi.nlm.nih.gov/pubmed/36444588
http://dx.doi.org/10.31557/APJCP.2022.23.11.3753
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