A three-step stacking capillary electrophoresis of field-amplified sample injection, sweeping, and micellar collapse for determination of dabigatran and its active metabolite in human plasma

A three-step stacking capillary electrophoresis (CE) composed of field-amplified sample injection, sweeping, and analyte focusing by micellar collapse (FASI-sweeping-AFMC) was developed to determine dabigatran (D) and its major active metabolite, dabigatran acyl-beta-d-glucuronide (DAG), in human pl...

Descripción completa

Detalles Bibliográficos
Autores principales: Cheng, Cheng-Wei, Feng, Wen-Han, Tang, Wei-Hua, Kou, Hwang-Shang, Wang, Chun-Chi, Wu, Shou-Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930998/
https://www.ncbi.nlm.nih.gov/pubmed/35647720
http://dx.doi.org/10.38212/2224-6614.3391
_version_ 1784889151959597056
author Cheng, Cheng-Wei
Feng, Wen-Han
Tang, Wei-Hua
Kou, Hwang-Shang
Wang, Chun-Chi
Wu, Shou-Mei
author_facet Cheng, Cheng-Wei
Feng, Wen-Han
Tang, Wei-Hua
Kou, Hwang-Shang
Wang, Chun-Chi
Wu, Shou-Mei
author_sort Cheng, Cheng-Wei
collection PubMed
description A three-step stacking capillary electrophoresis (CE) composed of field-amplified sample injection, sweeping, and analyte focusing by micellar collapse (FASI-sweeping-AFMC) was developed to determine dabigatran (D) and its major active metabolite, dabigatran acyl-beta-d-glucuronide (DAG), in human plasma. After optimization and validation, this novel approach was further applied to monitor 5 real samples, and the 25.2–186.8 ng mL(−1) D could be observed among those. Based on these results, the novel CE stacking strategy was successfully applied for the analysis of D and DAG in human plasma and could be served as a tool for clinical assays.
format Online
Article
Text
id pubmed-9930998
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Taiwan Food and Drug Administration
record_format MEDLINE/PubMed
spelling pubmed-99309982023-02-16 A three-step stacking capillary electrophoresis of field-amplified sample injection, sweeping, and micellar collapse for determination of dabigatran and its active metabolite in human plasma Cheng, Cheng-Wei Feng, Wen-Han Tang, Wei-Hua Kou, Hwang-Shang Wang, Chun-Chi Wu, Shou-Mei J Food Drug Anal Original Article A three-step stacking capillary electrophoresis (CE) composed of field-amplified sample injection, sweeping, and analyte focusing by micellar collapse (FASI-sweeping-AFMC) was developed to determine dabigatran (D) and its major active metabolite, dabigatran acyl-beta-d-glucuronide (DAG), in human plasma. After optimization and validation, this novel approach was further applied to monitor 5 real samples, and the 25.2–186.8 ng mL(−1) D could be observed among those. Based on these results, the novel CE stacking strategy was successfully applied for the analysis of D and DAG in human plasma and could be served as a tool for clinical assays. Taiwan Food and Drug Administration 2022-03-15 /pmc/articles/PMC9930998/ /pubmed/35647720 http://dx.doi.org/10.38212/2224-6614.3391 Text en © 2022 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Cheng, Cheng-Wei
Feng, Wen-Han
Tang, Wei-Hua
Kou, Hwang-Shang
Wang, Chun-Chi
Wu, Shou-Mei
A three-step stacking capillary electrophoresis of field-amplified sample injection, sweeping, and micellar collapse for determination of dabigatran and its active metabolite in human plasma
title A three-step stacking capillary electrophoresis of field-amplified sample injection, sweeping, and micellar collapse for determination of dabigatran and its active metabolite in human plasma
title_full A three-step stacking capillary electrophoresis of field-amplified sample injection, sweeping, and micellar collapse for determination of dabigatran and its active metabolite in human plasma
title_fullStr A three-step stacking capillary electrophoresis of field-amplified sample injection, sweeping, and micellar collapse for determination of dabigatran and its active metabolite in human plasma
title_full_unstemmed A three-step stacking capillary electrophoresis of field-amplified sample injection, sweeping, and micellar collapse for determination of dabigatran and its active metabolite in human plasma
title_short A three-step stacking capillary electrophoresis of field-amplified sample injection, sweeping, and micellar collapse for determination of dabigatran and its active metabolite in human plasma
title_sort three-step stacking capillary electrophoresis of field-amplified sample injection, sweeping, and micellar collapse for determination of dabigatran and its active metabolite in human plasma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930998/
https://www.ncbi.nlm.nih.gov/pubmed/35647720
http://dx.doi.org/10.38212/2224-6614.3391
work_keys_str_mv AT chengchengwei athreestepstackingcapillaryelectrophoresisoffieldamplifiedsampleinjectionsweepingandmicellarcollapsefordeterminationofdabigatrananditsactivemetaboliteinhumanplasma
AT fengwenhan athreestepstackingcapillaryelectrophoresisoffieldamplifiedsampleinjectionsweepingandmicellarcollapsefordeterminationofdabigatrananditsactivemetaboliteinhumanplasma
AT tangweihua athreestepstackingcapillaryelectrophoresisoffieldamplifiedsampleinjectionsweepingandmicellarcollapsefordeterminationofdabigatrananditsactivemetaboliteinhumanplasma
AT kouhwangshang athreestepstackingcapillaryelectrophoresisoffieldamplifiedsampleinjectionsweepingandmicellarcollapsefordeterminationofdabigatrananditsactivemetaboliteinhumanplasma
AT wangchunchi athreestepstackingcapillaryelectrophoresisoffieldamplifiedsampleinjectionsweepingandmicellarcollapsefordeterminationofdabigatrananditsactivemetaboliteinhumanplasma
AT wushoumei athreestepstackingcapillaryelectrophoresisoffieldamplifiedsampleinjectionsweepingandmicellarcollapsefordeterminationofdabigatrananditsactivemetaboliteinhumanplasma
AT chengchengwei threestepstackingcapillaryelectrophoresisoffieldamplifiedsampleinjectionsweepingandmicellarcollapsefordeterminationofdabigatrananditsactivemetaboliteinhumanplasma
AT fengwenhan threestepstackingcapillaryelectrophoresisoffieldamplifiedsampleinjectionsweepingandmicellarcollapsefordeterminationofdabigatrananditsactivemetaboliteinhumanplasma
AT tangweihua threestepstackingcapillaryelectrophoresisoffieldamplifiedsampleinjectionsweepingandmicellarcollapsefordeterminationofdabigatrananditsactivemetaboliteinhumanplasma
AT kouhwangshang threestepstackingcapillaryelectrophoresisoffieldamplifiedsampleinjectionsweepingandmicellarcollapsefordeterminationofdabigatrananditsactivemetaboliteinhumanplasma
AT wangchunchi threestepstackingcapillaryelectrophoresisoffieldamplifiedsampleinjectionsweepingandmicellarcollapsefordeterminationofdabigatrananditsactivemetaboliteinhumanplasma
AT wushoumei threestepstackingcapillaryelectrophoresisoffieldamplifiedsampleinjectionsweepingandmicellarcollapsefordeterminationofdabigatrananditsactivemetaboliteinhumanplasma

Ejemplares similares