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Impaired iron recycling from erythrocytes is an early hallmark of aging
Aging affects iron homeostasis, as evidenced by tissue iron loading and anemia in the elderly. Iron needs in mammals are met primarily by iron recycling from senescent red blood cells (RBCs), a task chiefly accomplished by splenic red pulp macrophages (RPMs) via erythrophagocytosis. Given that RPMs...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9931393/ https://www.ncbi.nlm.nih.gov/pubmed/36719185 http://dx.doi.org/10.7554/eLife.79196 |
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author | Slusarczyk, Patryk Mandal, Pratik Kumar Zurawska, Gabriela Niklewicz, Marta Chouhan, Komal Mahadeva, Raghunandan Jończy, Aneta Macias, Matylda Szybinska, Aleksandra Cybulska-Lubak, Magdalena Krawczyk, Olga Herman, Sylwia Mikula, Michal Serwa, Remigiusz Lenartowicz, Małgorzata Pokrzywa, Wojciech Mleczko-Sanecka, Katarzyna |
author_facet | Slusarczyk, Patryk Mandal, Pratik Kumar Zurawska, Gabriela Niklewicz, Marta Chouhan, Komal Mahadeva, Raghunandan Jończy, Aneta Macias, Matylda Szybinska, Aleksandra Cybulska-Lubak, Magdalena Krawczyk, Olga Herman, Sylwia Mikula, Michal Serwa, Remigiusz Lenartowicz, Małgorzata Pokrzywa, Wojciech Mleczko-Sanecka, Katarzyna |
author_sort | Slusarczyk, Patryk |
collection | PubMed |
description | Aging affects iron homeostasis, as evidenced by tissue iron loading and anemia in the elderly. Iron needs in mammals are met primarily by iron recycling from senescent red blood cells (RBCs), a task chiefly accomplished by splenic red pulp macrophages (RPMs) via erythrophagocytosis. Given that RPMs continuously process iron, their cellular functions might be susceptible to age-dependent decline, a possibility that has been unexplored to date. Here, we found that 10- to 11-month-old female mice exhibit iron loading in RPMs, largely attributable to a drop in iron exporter ferroportin, which diminishes their erythrophagocytosis capacity and lysosomal activity. Furthermore, we identified a loss of RPMs during aging, underlain by the combination of proteotoxic stress and iron-dependent cell death resembling ferroptosis. These impairments lead to the retention of senescent hemolytic RBCs in the spleen, and the formation of undegradable iron- and heme-rich extracellular protein aggregates, likely derived from ferroptotic RPMs. We further found that feeding mice an iron-reduced diet alleviates iron accumulation in RPMs, enhances their ability to clear erythrocytes, and reduces damage. Consequently, this diet ameliorates hemolysis of splenic RBCs and reduces the burden of protein aggregates, mildly increasing serum iron availability in aging mice. Taken together, we identified RPM collapse as an early hallmark of aging and demonstrated that dietary iron reduction improves iron turnover efficacy. |
format | Online Article Text |
id | pubmed-9931393 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-99313932023-02-16 Impaired iron recycling from erythrocytes is an early hallmark of aging Slusarczyk, Patryk Mandal, Pratik Kumar Zurawska, Gabriela Niklewicz, Marta Chouhan, Komal Mahadeva, Raghunandan Jończy, Aneta Macias, Matylda Szybinska, Aleksandra Cybulska-Lubak, Magdalena Krawczyk, Olga Herman, Sylwia Mikula, Michal Serwa, Remigiusz Lenartowicz, Małgorzata Pokrzywa, Wojciech Mleczko-Sanecka, Katarzyna eLife Cell Biology Aging affects iron homeostasis, as evidenced by tissue iron loading and anemia in the elderly. Iron needs in mammals are met primarily by iron recycling from senescent red blood cells (RBCs), a task chiefly accomplished by splenic red pulp macrophages (RPMs) via erythrophagocytosis. Given that RPMs continuously process iron, their cellular functions might be susceptible to age-dependent decline, a possibility that has been unexplored to date. Here, we found that 10- to 11-month-old female mice exhibit iron loading in RPMs, largely attributable to a drop in iron exporter ferroportin, which diminishes their erythrophagocytosis capacity and lysosomal activity. Furthermore, we identified a loss of RPMs during aging, underlain by the combination of proteotoxic stress and iron-dependent cell death resembling ferroptosis. These impairments lead to the retention of senescent hemolytic RBCs in the spleen, and the formation of undegradable iron- and heme-rich extracellular protein aggregates, likely derived from ferroptotic RPMs. We further found that feeding mice an iron-reduced diet alleviates iron accumulation in RPMs, enhances their ability to clear erythrocytes, and reduces damage. Consequently, this diet ameliorates hemolysis of splenic RBCs and reduces the burden of protein aggregates, mildly increasing serum iron availability in aging mice. Taken together, we identified RPM collapse as an early hallmark of aging and demonstrated that dietary iron reduction improves iron turnover efficacy. eLife Sciences Publications, Ltd 2023-01-31 /pmc/articles/PMC9931393/ /pubmed/36719185 http://dx.doi.org/10.7554/eLife.79196 Text en © 2023, Slusarczyk, Mandal et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Slusarczyk, Patryk Mandal, Pratik Kumar Zurawska, Gabriela Niklewicz, Marta Chouhan, Komal Mahadeva, Raghunandan Jończy, Aneta Macias, Matylda Szybinska, Aleksandra Cybulska-Lubak, Magdalena Krawczyk, Olga Herman, Sylwia Mikula, Michal Serwa, Remigiusz Lenartowicz, Małgorzata Pokrzywa, Wojciech Mleczko-Sanecka, Katarzyna Impaired iron recycling from erythrocytes is an early hallmark of aging |
title | Impaired iron recycling from erythrocytes is an early hallmark of aging |
title_full | Impaired iron recycling from erythrocytes is an early hallmark of aging |
title_fullStr | Impaired iron recycling from erythrocytes is an early hallmark of aging |
title_full_unstemmed | Impaired iron recycling from erythrocytes is an early hallmark of aging |
title_short | Impaired iron recycling from erythrocytes is an early hallmark of aging |
title_sort | impaired iron recycling from erythrocytes is an early hallmark of aging |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9931393/ https://www.ncbi.nlm.nih.gov/pubmed/36719185 http://dx.doi.org/10.7554/eLife.79196 |
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