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SPLIT-PIN software enabling confocal and super-resolution imaging with a virtually closed pinhole
In point-scanning microscopy, optical sectioning is achieved using a small aperture placed in front of the detector, i.e. the detection pinhole, which rejects the out-of-focus background. The maximum level of optical sectioning is theoretically obtained for the minimum size of the pinhole aperture,...
Autores principales: | Di Franco, Elisabetta, Costantino, Angelita, Cerutti, Elena, D’Amico, Morgana, Privitera, Anna P., Bianchini, Paolo, Vicidomini, Giuseppe, Gulisano, Massimo, Diaspro, Alberto, Lanzanò, Luca |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9931717/ https://www.ncbi.nlm.nih.gov/pubmed/36792719 http://dx.doi.org/10.1038/s41598-023-29951-9 |
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