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Moxibustion improves hypothalamus Aqp4 polarization in APP/PS1 mice: Evidence from spatial transcriptomics

Aquaporin-4 (AQP4) is highly polarized to perivascular astrocytic endfeet. Loss of AQP4 polarization is associated with many diseases. In Alzheimer’s disease (AD), AQP4 loses its normal location and thus reduces the clearance of amyloid-β plaques and tau protein. Clinical and experimental studies sh...

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Autores principales: Liu, Shuqing, Li, Hongying, Shen, Yuan, Zhu, Weikang, Wang, Yong, Wang, Junmeng, Zhang, Ning, Li, Chenyu, Xie, Lushuang, Wu, Qiaofeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9931733/
https://www.ncbi.nlm.nih.gov/pubmed/36819717
http://dx.doi.org/10.3389/fnagi.2023.1069155
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author Liu, Shuqing
Li, Hongying
Shen, Yuan
Zhu, Weikang
Wang, Yong
Wang, Junmeng
Zhang, Ning
Li, Chenyu
Xie, Lushuang
Wu, Qiaofeng
author_facet Liu, Shuqing
Li, Hongying
Shen, Yuan
Zhu, Weikang
Wang, Yong
Wang, Junmeng
Zhang, Ning
Li, Chenyu
Xie, Lushuang
Wu, Qiaofeng
author_sort Liu, Shuqing
collection PubMed
description Aquaporin-4 (AQP4) is highly polarized to perivascular astrocytic endfeet. Loss of AQP4 polarization is associated with many diseases. In Alzheimer’s disease (AD), AQP4 loses its normal location and thus reduces the clearance of amyloid-β plaques and tau protein. Clinical and experimental studies showed that moxibustion can improve the learning and memory abilities of AD. To explore whether moxibustion can affect the polarization of AQP4 around the blood-brain barrier (BBB), we used spatial transcriptomics (ST) to analyze the expression and polarization of Aqp4 in wild-type mice, APP/PS1 mice, and APP/PS1 mice intervened by moxibustion. The results showed that moxibustion improved the loss of abnormal polarization of AQP4 in APP/PS1 mice, especially in the hypothalamic BBB. Besides, the other 31 genes with Aqp4 as the core have similar depolarization in APP/PS1 mice, most of which are also membrane proteins. The majority of them have been reversed by moxibustion. At the same time, we employed the cerebrospinal fluid circulation gene set, which was found to be at a higher level in the group of APP/PS1 mice with moxibustion treatment. Finally, to further explore its mechanism, we analyzed the mitochondrial respiratory chain complex enzymes closely related to energy metabolism and found that moxibustion can significantly increase the expression of mitochondrial respiratory chain enzymes such as Cox6a2 in the hypothalamus, which could provide energy for mRNA transport. Our research shows that increasing the polarization of hypothalamic Aqp4 through mitochondrial energy supply may be an important target for moxibustion to improve cognitive impairment in APP/PS1 mice.
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spelling pubmed-99317332023-02-17 Moxibustion improves hypothalamus Aqp4 polarization in APP/PS1 mice: Evidence from spatial transcriptomics Liu, Shuqing Li, Hongying Shen, Yuan Zhu, Weikang Wang, Yong Wang, Junmeng Zhang, Ning Li, Chenyu Xie, Lushuang Wu, Qiaofeng Front Aging Neurosci Neuroscience Aquaporin-4 (AQP4) is highly polarized to perivascular astrocytic endfeet. Loss of AQP4 polarization is associated with many diseases. In Alzheimer’s disease (AD), AQP4 loses its normal location and thus reduces the clearance of amyloid-β plaques and tau protein. Clinical and experimental studies showed that moxibustion can improve the learning and memory abilities of AD. To explore whether moxibustion can affect the polarization of AQP4 around the blood-brain barrier (BBB), we used spatial transcriptomics (ST) to analyze the expression and polarization of Aqp4 in wild-type mice, APP/PS1 mice, and APP/PS1 mice intervened by moxibustion. The results showed that moxibustion improved the loss of abnormal polarization of AQP4 in APP/PS1 mice, especially in the hypothalamic BBB. Besides, the other 31 genes with Aqp4 as the core have similar depolarization in APP/PS1 mice, most of which are also membrane proteins. The majority of them have been reversed by moxibustion. At the same time, we employed the cerebrospinal fluid circulation gene set, which was found to be at a higher level in the group of APP/PS1 mice with moxibustion treatment. Finally, to further explore its mechanism, we analyzed the mitochondrial respiratory chain complex enzymes closely related to energy metabolism and found that moxibustion can significantly increase the expression of mitochondrial respiratory chain enzymes such as Cox6a2 in the hypothalamus, which could provide energy for mRNA transport. Our research shows that increasing the polarization of hypothalamic Aqp4 through mitochondrial energy supply may be an important target for moxibustion to improve cognitive impairment in APP/PS1 mice. Frontiers Media S.A. 2023-02-02 /pmc/articles/PMC9931733/ /pubmed/36819717 http://dx.doi.org/10.3389/fnagi.2023.1069155 Text en Copyright © 2023 Liu, Li, Shen, Zhu, Wang, Wang, Zhang, Li, Xie and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Liu, Shuqing
Li, Hongying
Shen, Yuan
Zhu, Weikang
Wang, Yong
Wang, Junmeng
Zhang, Ning
Li, Chenyu
Xie, Lushuang
Wu, Qiaofeng
Moxibustion improves hypothalamus Aqp4 polarization in APP/PS1 mice: Evidence from spatial transcriptomics
title Moxibustion improves hypothalamus Aqp4 polarization in APP/PS1 mice: Evidence from spatial transcriptomics
title_full Moxibustion improves hypothalamus Aqp4 polarization in APP/PS1 mice: Evidence from spatial transcriptomics
title_fullStr Moxibustion improves hypothalamus Aqp4 polarization in APP/PS1 mice: Evidence from spatial transcriptomics
title_full_unstemmed Moxibustion improves hypothalamus Aqp4 polarization in APP/PS1 mice: Evidence from spatial transcriptomics
title_short Moxibustion improves hypothalamus Aqp4 polarization in APP/PS1 mice: Evidence from spatial transcriptomics
title_sort moxibustion improves hypothalamus aqp4 polarization in app/ps1 mice: evidence from spatial transcriptomics
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9931733/
https://www.ncbi.nlm.nih.gov/pubmed/36819717
http://dx.doi.org/10.3389/fnagi.2023.1069155
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