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CRISPR/Cas9-mediated targeted knock-in of large constructs using nocodazole and RNase HII
On-target integration of large cassettes via homology-directed repair (HDR) has several applications. However, the HDR-mediated targeted knock-in suffered from low efficiency. In this study, we made several large plasmids (12.1–13.4 kb) which included the CRISPR/Cas9 system along with a puromycin tr...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9931768/ https://www.ncbi.nlm.nih.gov/pubmed/36792645 http://dx.doi.org/10.1038/s41598-023-29789-1 |