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The power of light: Impact on the performance of biocontrol agents under minimal nutrient conditions

BACKGROUND: The spectral distribution of light (different wavelength) has recently been identified as an important factor in the dynamics and function of leaf-associated microbes. This study investigated the impact of different wavelength on three commercial biocontrol agents (BCA): Bacillus amyloli...

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Autores principales: Karlsson, Maria E., Hellström, Maria, Flöhr, Adam, Bergstrand, Karl-Johan, Alsanius, Beatrix W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932321/
https://www.ncbi.nlm.nih.gov/pubmed/36819051
http://dx.doi.org/10.3389/fmicb.2023.1087639
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author Karlsson, Maria E.
Hellström, Maria
Flöhr, Adam
Bergstrand, Karl-Johan
Alsanius, Beatrix W.
author_facet Karlsson, Maria E.
Hellström, Maria
Flöhr, Adam
Bergstrand, Karl-Johan
Alsanius, Beatrix W.
author_sort Karlsson, Maria E.
collection PubMed
description BACKGROUND: The spectral distribution of light (different wavelength) has recently been identified as an important factor in the dynamics and function of leaf-associated microbes. This study investigated the impact of different wavelength on three commercial biocontrol agents (BCA): Bacillus amyloliquefaciens (BA), Pseudomonas chlororaphis (PC), and Streptomyces griseoviridis (SG). METHODS: The impact of light exposure on sole carbon source utilization, biofilm formation, and biosurfactant production by the selected BCA was studied using phenotypic microarray (PM) including 190 sole carbon sources (OmniLog®, PM panels 1 and 2). The BCA were exposed to five monochromatic light conditions (420, 460, 530, 630, and 660 nm) and darkness during incubation, at an intensity of 50 μmol m(−2) s(−1). RESULTS: Light exposure together with specific carbon source increased respiration in all three BCA. Different wavelengths of light influenced sole carbon utilization for the different BCA, with BA and PC showing increased respiration when exposed to wavelengths within the blue spectrum (420 and 460 nm) while respiration of selected carbon sources by SG increased in the presence of red light (630 and 660 nm). Only one carbon source (capric acid) generated biosurfactant production in all three BCA. A combination of specific wavelength of light and sole carbon source increased biofilm formation in all three BCA. BA showed significantly higher biofilm formation when exposed to blue (460 nm) and green (530 nm) light and propagated in D-sucrose, D-fructose, and dulcitol. PC showed higher biofilm formation when exposed to blue light. Biofilm formation by SG increased when exposed to red light (630 nm) and propagated in citraconic acid. CONCLUSION: To increase attachment and success in BCA introduced into the phyllosphere, a suitable combination of light quality and nutrient conditions could be used.
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spelling pubmed-99323212023-02-17 The power of light: Impact on the performance of biocontrol agents under minimal nutrient conditions Karlsson, Maria E. Hellström, Maria Flöhr, Adam Bergstrand, Karl-Johan Alsanius, Beatrix W. Front Microbiol Microbiology BACKGROUND: The spectral distribution of light (different wavelength) has recently been identified as an important factor in the dynamics and function of leaf-associated microbes. This study investigated the impact of different wavelength on three commercial biocontrol agents (BCA): Bacillus amyloliquefaciens (BA), Pseudomonas chlororaphis (PC), and Streptomyces griseoviridis (SG). METHODS: The impact of light exposure on sole carbon source utilization, biofilm formation, and biosurfactant production by the selected BCA was studied using phenotypic microarray (PM) including 190 sole carbon sources (OmniLog®, PM panels 1 and 2). The BCA were exposed to five monochromatic light conditions (420, 460, 530, 630, and 660 nm) and darkness during incubation, at an intensity of 50 μmol m(−2) s(−1). RESULTS: Light exposure together with specific carbon source increased respiration in all three BCA. Different wavelengths of light influenced sole carbon utilization for the different BCA, with BA and PC showing increased respiration when exposed to wavelengths within the blue spectrum (420 and 460 nm) while respiration of selected carbon sources by SG increased in the presence of red light (630 and 660 nm). Only one carbon source (capric acid) generated biosurfactant production in all three BCA. A combination of specific wavelength of light and sole carbon source increased biofilm formation in all three BCA. BA showed significantly higher biofilm formation when exposed to blue (460 nm) and green (530 nm) light and propagated in D-sucrose, D-fructose, and dulcitol. PC showed higher biofilm formation when exposed to blue light. Biofilm formation by SG increased when exposed to red light (630 nm) and propagated in citraconic acid. CONCLUSION: To increase attachment and success in BCA introduced into the phyllosphere, a suitable combination of light quality and nutrient conditions could be used. Frontiers Media S.A. 2023-02-02 /pmc/articles/PMC9932321/ /pubmed/36819051 http://dx.doi.org/10.3389/fmicb.2023.1087639 Text en Copyright © 2023 Karlsson, Hellström, Flöhr, Bergstrand and Alsanius. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Karlsson, Maria E.
Hellström, Maria
Flöhr, Adam
Bergstrand, Karl-Johan
Alsanius, Beatrix W.
The power of light: Impact on the performance of biocontrol agents under minimal nutrient conditions
title The power of light: Impact on the performance of biocontrol agents under minimal nutrient conditions
title_full The power of light: Impact on the performance of biocontrol agents under minimal nutrient conditions
title_fullStr The power of light: Impact on the performance of biocontrol agents under minimal nutrient conditions
title_full_unstemmed The power of light: Impact on the performance of biocontrol agents under minimal nutrient conditions
title_short The power of light: Impact on the performance of biocontrol agents under minimal nutrient conditions
title_sort power of light: impact on the performance of biocontrol agents under minimal nutrient conditions
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932321/
https://www.ncbi.nlm.nih.gov/pubmed/36819051
http://dx.doi.org/10.3389/fmicb.2023.1087639
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