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Prognostic biomarker NEIL3 and its association with immune infiltration in renal clear cell carcinoma

BACKGROUND: Kidney renal clear cell carcinoma (KIRC) is a malignant tumor with a high degree of immune infiltration. Identifying immune biomarkers is essential for the treatment of KIRC. Studies have identified the potential of NEIL3 to modulate the immune microenvironment and promote tumor progress...

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Autores principales: Sun, Xiaomei, Liu, Pengfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932331/
https://www.ncbi.nlm.nih.gov/pubmed/36816967
http://dx.doi.org/10.3389/fonc.2023.1073941
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author Sun, Xiaomei
Liu, Pengfei
author_facet Sun, Xiaomei
Liu, Pengfei
author_sort Sun, Xiaomei
collection PubMed
description BACKGROUND: Kidney renal clear cell carcinoma (KIRC) is a malignant tumor with a high degree of immune infiltration. Identifying immune biomarkers is essential for the treatment of KIRC. Studies have identified the potential of NEIL3 to modulate the immune microenvironment and promote tumor progression. However, the role of NEIL3 in KIRC remains uncertain. This study was to investigate the effect of NEIL3 on the prognosis and immune infiltration of patients with KIRC. METHODS: TCGA and GEO databases were used to study the expression of NEIL3 in KIRC. Cox regression analysis was used to examine the relationship between the expression of NEIL3 and clinicopathological variables and survival. Furthermore, Gene Set Cancer Analysis (GSCA) was applied to study the impact of NEIL3 methylation on outcomes of KIRC. Through gene ontology (GO) and Gene set enrichment (GSEA) analysis, the biological processes and signal pathways related to NEIL3 expression were identified. In addition, immune infiltration analysis was conducted via CIBERSORT analysis, ssGSEA analysis and TISIDB database. RESULTS: NEIL3 was overexpressed in KIRC, and it was significantly related with histologic grade, pathologic stage, T stage, M stage, and vital status of KIRC patients (P < 0.001). The expression of NEIL3 was associated with worse outcomes. Univariate and multivariate Cox analysis showed that NEIL3 may be an indicator of adverse outcomes in KIRC. GSEA analysis revealed that NEIL3 may be involved in signal pathways including cell cycle, DNA replication, mismatch repair, P53 signal pathway, and antigen processing and presentation. In addition, immune infiltration analysis showed a positive correlation between NEIL3 expression and multiple immune cells (activated CD8 T cells, activated dendritic cells, myeloid-derived suppressor cells, follicular helper T cells, and regulatory T cells) and immunoinhibitors (PD1, CTLA4, LAG3, TIGHT, IL10, and CD96). CONCLUSION: NEIL3 is a potential independent biomarker of KIRC, which is relevant to immune infiltration.
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spelling pubmed-99323312023-02-17 Prognostic biomarker NEIL3 and its association with immune infiltration in renal clear cell carcinoma Sun, Xiaomei Liu, Pengfei Front Oncol Oncology BACKGROUND: Kidney renal clear cell carcinoma (KIRC) is a malignant tumor with a high degree of immune infiltration. Identifying immune biomarkers is essential for the treatment of KIRC. Studies have identified the potential of NEIL3 to modulate the immune microenvironment and promote tumor progression. However, the role of NEIL3 in KIRC remains uncertain. This study was to investigate the effect of NEIL3 on the prognosis and immune infiltration of patients with KIRC. METHODS: TCGA and GEO databases were used to study the expression of NEIL3 in KIRC. Cox regression analysis was used to examine the relationship between the expression of NEIL3 and clinicopathological variables and survival. Furthermore, Gene Set Cancer Analysis (GSCA) was applied to study the impact of NEIL3 methylation on outcomes of KIRC. Through gene ontology (GO) and Gene set enrichment (GSEA) analysis, the biological processes and signal pathways related to NEIL3 expression were identified. In addition, immune infiltration analysis was conducted via CIBERSORT analysis, ssGSEA analysis and TISIDB database. RESULTS: NEIL3 was overexpressed in KIRC, and it was significantly related with histologic grade, pathologic stage, T stage, M stage, and vital status of KIRC patients (P < 0.001). The expression of NEIL3 was associated with worse outcomes. Univariate and multivariate Cox analysis showed that NEIL3 may be an indicator of adverse outcomes in KIRC. GSEA analysis revealed that NEIL3 may be involved in signal pathways including cell cycle, DNA replication, mismatch repair, P53 signal pathway, and antigen processing and presentation. In addition, immune infiltration analysis showed a positive correlation between NEIL3 expression and multiple immune cells (activated CD8 T cells, activated dendritic cells, myeloid-derived suppressor cells, follicular helper T cells, and regulatory T cells) and immunoinhibitors (PD1, CTLA4, LAG3, TIGHT, IL10, and CD96). CONCLUSION: NEIL3 is a potential independent biomarker of KIRC, which is relevant to immune infiltration. Frontiers Media S.A. 2023-02-02 /pmc/articles/PMC9932331/ /pubmed/36816967 http://dx.doi.org/10.3389/fonc.2023.1073941 Text en Copyright © 2023 Sun and Liu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Sun, Xiaomei
Liu, Pengfei
Prognostic biomarker NEIL3 and its association with immune infiltration in renal clear cell carcinoma
title Prognostic biomarker NEIL3 and its association with immune infiltration in renal clear cell carcinoma
title_full Prognostic biomarker NEIL3 and its association with immune infiltration in renal clear cell carcinoma
title_fullStr Prognostic biomarker NEIL3 and its association with immune infiltration in renal clear cell carcinoma
title_full_unstemmed Prognostic biomarker NEIL3 and its association with immune infiltration in renal clear cell carcinoma
title_short Prognostic biomarker NEIL3 and its association with immune infiltration in renal clear cell carcinoma
title_sort prognostic biomarker neil3 and its association with immune infiltration in renal clear cell carcinoma
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932331/
https://www.ncbi.nlm.nih.gov/pubmed/36816967
http://dx.doi.org/10.3389/fonc.2023.1073941
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