Cargando…

Acute phase proteins levels in horses, after a single carbohydrate overload, associated with cecal alkalinization

INTRODUCTION: Horses submitted to carbohydrate overload can develop laminitis due to changes in cecal pH and microbiota, followed by an increase in transmural absorption of luminal content, including bacterial toxins. In response to acute injury there is hepatic overproduction of several proteins kn...

Descripción completa

Detalles Bibliográficos
Autores principales: Peixoto Rabelo, Isabela, Barroco de Paula, Vanessa, Carvalho Bustamante, Caio, Santana, André Marcos, Gomes da Silva, Daniela, Baldassi, Amanda Cristina, Canola, Paulo Aléscio, Araújo Valadão, Carlos Augusto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932335/
https://www.ncbi.nlm.nih.gov/pubmed/36816195
http://dx.doi.org/10.3389/fvets.2023.1043656
_version_ 1784889434105184256
author Peixoto Rabelo, Isabela
Barroco de Paula, Vanessa
Carvalho Bustamante, Caio
Santana, André Marcos
Gomes da Silva, Daniela
Baldassi, Amanda Cristina
Canola, Paulo Aléscio
Araújo Valadão, Carlos Augusto
author_facet Peixoto Rabelo, Isabela
Barroco de Paula, Vanessa
Carvalho Bustamante, Caio
Santana, André Marcos
Gomes da Silva, Daniela
Baldassi, Amanda Cristina
Canola, Paulo Aléscio
Araújo Valadão, Carlos Augusto
author_sort Peixoto Rabelo, Isabela
collection PubMed
description INTRODUCTION: Horses submitted to carbohydrate overload can develop laminitis due to changes in cecal pH and microbiota, followed by an increase in transmural absorption of luminal content, including bacterial toxins. In response to acute injury there is hepatic overproduction of several proteins known as acute phase proteins (APP). Few studies have evaluated protein fractionation to characterize the inflammatory response in acute laminitis. The aim of this study was to test the viability of an experimental model to induce acute laminitis, using a single carbohydrate overload, and the influence of a buffering solution on the development of the disease; also, study the kinetics of APP during acute laminitis, as well as the correlation between these proteins and clinical signs associated to this syndrome. METHODS: Ten healthy horses were divided in a factorial and randomized way into four groups (n = 5): control group (CG), starch group (SG), buffer group (BG), and starch C buffer group (SBG). They were evaluated at seven times (T0h, T4h, T8h, T12h, T24h, T48h, and T72h), which included clinical evaluation and blood sample collection. Total serum protein and albumin concentrations were determined by colorimetry and the other APP by polyacrylamide gel electrophoresis containing sodium dodecyl sulfate and commercial ELISA kits. Data were analyzed by two-way ANOVA, followed by Tukey's test (p < 0.05). The correlation between clinical signs and APP were verified using the Pearson's correlation coefficient. RESULTS AND DISCUSSION: 40% of the animals from SG and 60% from SBG developed clinical laminitis. A single administration of buffer solution was not able to prevent clinical signs of laminitis. There was no difference between groups on total serum protein, albumin, serum amyloid A and C-reactive protein concentrations (p > 0.05). Transferrin, considered a negative APP, showed a positive response pattern in SG and SBG. Ceruloplasmin had a positive correlation with Obel grade, heart rate on animals from SGB and number of steps on horses submitted to starch overload (SG and SBG). Ceruloplasmin, α-1-antitrypsin and haptoglobin concentrations increased in SBG, suggesting an inflammatory response in animals of this group. Changes in clinical parameters were also more evident in the SBG, corroborating the protein fractionation findings.
format Online
Article
Text
id pubmed-9932335
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-99323352023-02-17 Acute phase proteins levels in horses, after a single carbohydrate overload, associated with cecal alkalinization Peixoto Rabelo, Isabela Barroco de Paula, Vanessa Carvalho Bustamante, Caio Santana, André Marcos Gomes da Silva, Daniela Baldassi, Amanda Cristina Canola, Paulo Aléscio Araújo Valadão, Carlos Augusto Front Vet Sci Veterinary Science INTRODUCTION: Horses submitted to carbohydrate overload can develop laminitis due to changes in cecal pH and microbiota, followed by an increase in transmural absorption of luminal content, including bacterial toxins. In response to acute injury there is hepatic overproduction of several proteins known as acute phase proteins (APP). Few studies have evaluated protein fractionation to characterize the inflammatory response in acute laminitis. The aim of this study was to test the viability of an experimental model to induce acute laminitis, using a single carbohydrate overload, and the influence of a buffering solution on the development of the disease; also, study the kinetics of APP during acute laminitis, as well as the correlation between these proteins and clinical signs associated to this syndrome. METHODS: Ten healthy horses were divided in a factorial and randomized way into four groups (n = 5): control group (CG), starch group (SG), buffer group (BG), and starch C buffer group (SBG). They were evaluated at seven times (T0h, T4h, T8h, T12h, T24h, T48h, and T72h), which included clinical evaluation and blood sample collection. Total serum protein and albumin concentrations were determined by colorimetry and the other APP by polyacrylamide gel electrophoresis containing sodium dodecyl sulfate and commercial ELISA kits. Data were analyzed by two-way ANOVA, followed by Tukey's test (p < 0.05). The correlation between clinical signs and APP were verified using the Pearson's correlation coefficient. RESULTS AND DISCUSSION: 40% of the animals from SG and 60% from SBG developed clinical laminitis. A single administration of buffer solution was not able to prevent clinical signs of laminitis. There was no difference between groups on total serum protein, albumin, serum amyloid A and C-reactive protein concentrations (p > 0.05). Transferrin, considered a negative APP, showed a positive response pattern in SG and SBG. Ceruloplasmin had a positive correlation with Obel grade, heart rate on animals from SGB and number of steps on horses submitted to starch overload (SG and SBG). Ceruloplasmin, α-1-antitrypsin and haptoglobin concentrations increased in SBG, suggesting an inflammatory response in animals of this group. Changes in clinical parameters were also more evident in the SBG, corroborating the protein fractionation findings. Frontiers Media S.A. 2023-02-02 /pmc/articles/PMC9932335/ /pubmed/36816195 http://dx.doi.org/10.3389/fvets.2023.1043656 Text en Copyright © 2023 Peixoto Rabelo, Barroco de Paula, Carvalho Bustamante, Santana, Gomes da Silva, Baldassi, Canola and Araújo Valadão. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Peixoto Rabelo, Isabela
Barroco de Paula, Vanessa
Carvalho Bustamante, Caio
Santana, André Marcos
Gomes da Silva, Daniela
Baldassi, Amanda Cristina
Canola, Paulo Aléscio
Araújo Valadão, Carlos Augusto
Acute phase proteins levels in horses, after a single carbohydrate overload, associated with cecal alkalinization
title Acute phase proteins levels in horses, after a single carbohydrate overload, associated with cecal alkalinization
title_full Acute phase proteins levels in horses, after a single carbohydrate overload, associated with cecal alkalinization
title_fullStr Acute phase proteins levels in horses, after a single carbohydrate overload, associated with cecal alkalinization
title_full_unstemmed Acute phase proteins levels in horses, after a single carbohydrate overload, associated with cecal alkalinization
title_short Acute phase proteins levels in horses, after a single carbohydrate overload, associated with cecal alkalinization
title_sort acute phase proteins levels in horses, after a single carbohydrate overload, associated with cecal alkalinization
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932335/
https://www.ncbi.nlm.nih.gov/pubmed/36816195
http://dx.doi.org/10.3389/fvets.2023.1043656
work_keys_str_mv AT peixotorabeloisabela acutephaseproteinslevelsinhorsesafterasinglecarbohydrateoverloadassociatedwithcecalalkalinization
AT barrocodepaulavanessa acutephaseproteinslevelsinhorsesafterasinglecarbohydrateoverloadassociatedwithcecalalkalinization
AT carvalhobustamantecaio acutephaseproteinslevelsinhorsesafterasinglecarbohydrateoverloadassociatedwithcecalalkalinization
AT santanaandremarcos acutephaseproteinslevelsinhorsesafterasinglecarbohydrateoverloadassociatedwithcecalalkalinization
AT gomesdasilvadaniela acutephaseproteinslevelsinhorsesafterasinglecarbohydrateoverloadassociatedwithcecalalkalinization
AT baldassiamandacristina acutephaseproteinslevelsinhorsesafterasinglecarbohydrateoverloadassociatedwithcecalalkalinization
AT canolapauloalescio acutephaseproteinslevelsinhorsesafterasinglecarbohydrateoverloadassociatedwithcecalalkalinization
AT araujovaladaocarlosaugusto acutephaseproteinslevelsinhorsesafterasinglecarbohydrateoverloadassociatedwithcecalalkalinization