Protocol to generate induced trophoblast stem cells from embryonic stem cells in mice

Conversion of trophectoderm (TE)-derived trophoblast stem cells (TSCs) from inner-cell-mass-derived embryonic stem cells (ESCs) in mice is difficult to achieve naturally. Here, we introduce a reliable and repeatable protocol to generate induced TSCs (iTSCs) from ESCs via a Tet-on system in vitro. Th...

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Detalles Bibliográficos
Autores principales: Zhao, Yiding, Wang, Qing, Zhang, Wenhao, Jia, Qingshen, Gao, Qian, Shuai, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932551/
https://www.ncbi.nlm.nih.gov/pubmed/36853659
http://dx.doi.org/10.1016/j.xpro.2023.102092
Descripción
Sumario:Conversion of trophectoderm (TE)-derived trophoblast stem cells (TSCs) from inner-cell-mass-derived embryonic stem cells (ESCs) in mice is difficult to achieve naturally. Here, we introduce a reliable and repeatable protocol to generate induced TSCs (iTSCs) from ESCs via a Tet-on system in vitro. The iTSCs show typical TSC properties and have the potential to differentiate into syncytiotrophoblast cells (STCs) and trophoblast giant cells (TGCs). This cell fate transition provides a general platform to robustly investigate the mechanisms underlying TE specification. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).(1)

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