Protocol to generate induced trophoblast stem cells from embryonic stem cells in mice

Conversion of trophectoderm (TE)-derived trophoblast stem cells (TSCs) from inner-cell-mass-derived embryonic stem cells (ESCs) in mice is difficult to achieve naturally. Here, we introduce a reliable and repeatable protocol to generate induced TSCs (iTSCs) from ESCs via a Tet-on system in vitro. Th...

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Detalles Bibliográficos
Autores principales: Zhao, Yiding, Wang, Qing, Zhang, Wenhao, Jia, Qingshen, Gao, Qian, Shuai, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932551/
https://www.ncbi.nlm.nih.gov/pubmed/36853659
http://dx.doi.org/10.1016/j.xpro.2023.102092
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author Zhao, Yiding
Wang, Qing
Zhang, Wenhao
Jia, Qingshen
Gao, Qian
Shuai, Ling
author_facet Zhao, Yiding
Wang, Qing
Zhang, Wenhao
Jia, Qingshen
Gao, Qian
Shuai, Ling
author_sort Zhao, Yiding
collection PubMed
description Conversion of trophectoderm (TE)-derived trophoblast stem cells (TSCs) from inner-cell-mass-derived embryonic stem cells (ESCs) in mice is difficult to achieve naturally. Here, we introduce a reliable and repeatable protocol to generate induced TSCs (iTSCs) from ESCs via a Tet-on system in vitro. The iTSCs show typical TSC properties and have the potential to differentiate into syncytiotrophoblast cells (STCs) and trophoblast giant cells (TGCs). This cell fate transition provides a general platform to robustly investigate the mechanisms underlying TE specification. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).(1)
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spelling pubmed-99325512023-02-17 Protocol to generate induced trophoblast stem cells from embryonic stem cells in mice Zhao, Yiding Wang, Qing Zhang, Wenhao Jia, Qingshen Gao, Qian Shuai, Ling STAR Protoc Protocol Conversion of trophectoderm (TE)-derived trophoblast stem cells (TSCs) from inner-cell-mass-derived embryonic stem cells (ESCs) in mice is difficult to achieve naturally. Here, we introduce a reliable and repeatable protocol to generate induced TSCs (iTSCs) from ESCs via a Tet-on system in vitro. The iTSCs show typical TSC properties and have the potential to differentiate into syncytiotrophoblast cells (STCs) and trophoblast giant cells (TGCs). This cell fate transition provides a general platform to robustly investigate the mechanisms underlying TE specification. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).(1) Elsevier 2023-02-03 /pmc/articles/PMC9932551/ /pubmed/36853659 http://dx.doi.org/10.1016/j.xpro.2023.102092 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Zhao, Yiding
Wang, Qing
Zhang, Wenhao
Jia, Qingshen
Gao, Qian
Shuai, Ling
Protocol to generate induced trophoblast stem cells from embryonic stem cells in mice
title Protocol to generate induced trophoblast stem cells from embryonic stem cells in mice
title_full Protocol to generate induced trophoblast stem cells from embryonic stem cells in mice
title_fullStr Protocol to generate induced trophoblast stem cells from embryonic stem cells in mice
title_full_unstemmed Protocol to generate induced trophoblast stem cells from embryonic stem cells in mice
title_short Protocol to generate induced trophoblast stem cells from embryonic stem cells in mice
title_sort protocol to generate induced trophoblast stem cells from embryonic stem cells in mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932551/
https://www.ncbi.nlm.nih.gov/pubmed/36853659
http://dx.doi.org/10.1016/j.xpro.2023.102092
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