Cargando…
Protocol for mitochondrial isolation and sub-cellular localization assay for mitochondrial proteins
Here, we provide a protocol to isolate mitochondria from cultured cells and extract differently located mitochondrial proteins. We detail steps to separate both integral and peripheral membrane proteins from soluble proteins using sonication. We describe the separation of integral membrane proteins...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932554/ https://www.ncbi.nlm.nih.gov/pubmed/36853693 http://dx.doi.org/10.1016/j.xpro.2023.102088 |