Detecting RNA-protein proximity at DNA double-strand breaks using combined fluorescence in situ hybridization with proximity ligation assay

RNA transcribed at DNA double-strand breaks (DSBs) contributes to accurate DNA repair. Here, using the repair factors 53BP1 and TIRR as examples, we combine the fluorescence in situ hybridization (FISH) and proximity ligation assay (PLA) techniques to determine protein proximity to DSB-transcribed R...

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Detalles Bibliográficos
Autores principales: Alagia, Adele, Ketley, Ruth F., Gullerova, Monika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932567/
https://www.ncbi.nlm.nih.gov/pubmed/36825808
http://dx.doi.org/10.1016/j.xpro.2023.102096

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932567/
https://www.ncbi.nlm.nih.gov/pubmed/36825808
http://dx.doi.org/10.1016/j.xpro.2023.102096

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