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PM(2.5) induce lifespan reduction, insulin/IGF-1 signaling pathway disruption and lipid metabolism disorder in Caenorhabditis elegans
INTRODUCTION: Exposure to fine particulate matter (PM), especially PM(2.5), can induce various adverse health effects in populations, including diseases and premature death, but the mechanism of its toxicity is largely unknown. METHODS: Water-soluble components of PM(2.5) (WS-PM(2.5)) were collected...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932997/ https://www.ncbi.nlm.nih.gov/pubmed/36817915 http://dx.doi.org/10.3389/fpubh.2023.1055175 |
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author | Zhang, Wenjing Li, Zinan Li, Guojun Kong, Ling Jing, Haiming Zhang, Nan Ning, Junyu Gao, Shan Zhang, Yong Wang, Xinyu Tao, Jing |
author_facet | Zhang, Wenjing Li, Zinan Li, Guojun Kong, Ling Jing, Haiming Zhang, Nan Ning, Junyu Gao, Shan Zhang, Yong Wang, Xinyu Tao, Jing |
author_sort | Zhang, Wenjing |
collection | PubMed |
description | INTRODUCTION: Exposure to fine particulate matter (PM), especially PM(2.5), can induce various adverse health effects in populations, including diseases and premature death, but the mechanism of its toxicity is largely unknown. METHODS: Water-soluble components of PM(2.5) (WS-PM(2.5)) were collected in the north of China in winter, and combined in two groups with the final concentrations of 94 μg/mL (C(L) group, AQI ≤ 100) and 119 μg/mL (C(H) group, 100 < AQI ≤ 200), respectively. The acute and long-term toxic effects of WS-PM(2.5) samples were evaluated in several aspects such as development, lifespan, healthspan (locomotion behavior, heat stress tolerance, lipofucin). DAF mutants and genes were applied to verify the action of IIS pathway in WS-PM(2.5) induced-effects. RNA-Sequencing was performed to elucidate the molecular mechanisms, as well as ROS production and Oil red O staining were also served as means of mechanism exploration. RESULTS: Body length and lifespan were shortened by exposure to WS-PM(2.5). Healthspan of nematodes revealed adverse effects evaluated by head thrash, body bend, pharyngeal pump, as well as intestinal lipofuscin accumulation and survival time under heat stress. The abbreviated lifespan of daf-2(e1370) strain and reduced expression level of daf-16 and hsp-16.2 indicated that IIS pathway might be involved in the mechanism. Thirty-five abnormally expressed genes screened out by RNA-Sequencing techniques, were functionally enriched in lipid/lipid metabolism and transport, and may contribute substantially to the regulation of PM(2.5) induced adverse effects in nematodes. CONCLUSION: WS-PM(2.5) exposure induce varying degrees of toxic effects, such as body development, shorten lifespan and healthspan. The IIS pathway and lipid metabolism/transport were disturbed by WS-PM(2.5) during WS-PM(2.5) exposure, suggesting their regulatory role in lifespan determination. |
format | Online Article Text |
id | pubmed-9932997 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99329972023-02-17 PM(2.5) induce lifespan reduction, insulin/IGF-1 signaling pathway disruption and lipid metabolism disorder in Caenorhabditis elegans Zhang, Wenjing Li, Zinan Li, Guojun Kong, Ling Jing, Haiming Zhang, Nan Ning, Junyu Gao, Shan Zhang, Yong Wang, Xinyu Tao, Jing Front Public Health Public Health INTRODUCTION: Exposure to fine particulate matter (PM), especially PM(2.5), can induce various adverse health effects in populations, including diseases and premature death, but the mechanism of its toxicity is largely unknown. METHODS: Water-soluble components of PM(2.5) (WS-PM(2.5)) were collected in the north of China in winter, and combined in two groups with the final concentrations of 94 μg/mL (C(L) group, AQI ≤ 100) and 119 μg/mL (C(H) group, 100 < AQI ≤ 200), respectively. The acute and long-term toxic effects of WS-PM(2.5) samples were evaluated in several aspects such as development, lifespan, healthspan (locomotion behavior, heat stress tolerance, lipofucin). DAF mutants and genes were applied to verify the action of IIS pathway in WS-PM(2.5) induced-effects. RNA-Sequencing was performed to elucidate the molecular mechanisms, as well as ROS production and Oil red O staining were also served as means of mechanism exploration. RESULTS: Body length and lifespan were shortened by exposure to WS-PM(2.5). Healthspan of nematodes revealed adverse effects evaluated by head thrash, body bend, pharyngeal pump, as well as intestinal lipofuscin accumulation and survival time under heat stress. The abbreviated lifespan of daf-2(e1370) strain and reduced expression level of daf-16 and hsp-16.2 indicated that IIS pathway might be involved in the mechanism. Thirty-five abnormally expressed genes screened out by RNA-Sequencing techniques, were functionally enriched in lipid/lipid metabolism and transport, and may contribute substantially to the regulation of PM(2.5) induced adverse effects in nematodes. CONCLUSION: WS-PM(2.5) exposure induce varying degrees of toxic effects, such as body development, shorten lifespan and healthspan. The IIS pathway and lipid metabolism/transport were disturbed by WS-PM(2.5) during WS-PM(2.5) exposure, suggesting their regulatory role in lifespan determination. Frontiers Media S.A. 2023-02-02 /pmc/articles/PMC9932997/ /pubmed/36817915 http://dx.doi.org/10.3389/fpubh.2023.1055175 Text en Copyright © 2023 Zhang, Li, Li, Kong, Jing, Zhang, Ning, Gao, Zhang, Wang and Tao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Public Health Zhang, Wenjing Li, Zinan Li, Guojun Kong, Ling Jing, Haiming Zhang, Nan Ning, Junyu Gao, Shan Zhang, Yong Wang, Xinyu Tao, Jing PM(2.5) induce lifespan reduction, insulin/IGF-1 signaling pathway disruption and lipid metabolism disorder in Caenorhabditis elegans |
title | PM(2.5) induce lifespan reduction, insulin/IGF-1 signaling pathway disruption and lipid metabolism disorder in Caenorhabditis elegans |
title_full | PM(2.5) induce lifespan reduction, insulin/IGF-1 signaling pathway disruption and lipid metabolism disorder in Caenorhabditis elegans |
title_fullStr | PM(2.5) induce lifespan reduction, insulin/IGF-1 signaling pathway disruption and lipid metabolism disorder in Caenorhabditis elegans |
title_full_unstemmed | PM(2.5) induce lifespan reduction, insulin/IGF-1 signaling pathway disruption and lipid metabolism disorder in Caenorhabditis elegans |
title_short | PM(2.5) induce lifespan reduction, insulin/IGF-1 signaling pathway disruption and lipid metabolism disorder in Caenorhabditis elegans |
title_sort | pm(2.5) induce lifespan reduction, insulin/igf-1 signaling pathway disruption and lipid metabolism disorder in caenorhabditis elegans |
topic | Public Health |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932997/ https://www.ncbi.nlm.nih.gov/pubmed/36817915 http://dx.doi.org/10.3389/fpubh.2023.1055175 |
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