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Self-assembled multiepitope nanovaccine based on NoV P particles induces effective and lasting protection against H3N2 influenza virus
Current seasonal influenza vaccines confer only limited coverage of virus strains due to the frequent genetic and antigenic variability of influenza virus (IV). Epitope vaccines that accurately target conserved domains provide a promising approach to increase the breadth of protection; however, poor...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tsinghua University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933037/ https://www.ncbi.nlm.nih.gov/pubmed/36820263 http://dx.doi.org/10.1007/s12274-023-5395-6 |
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author | Nie, Jiaojiao Wang, Qingyu Jin, Shenghui Yao, Xin Xu, Lipeng Chang, Yaotian Ding, Fan Li, Zeyu Sun, Lulu Shi, Yuhua Shan, Yaming |
author_facet | Nie, Jiaojiao Wang, Qingyu Jin, Shenghui Yao, Xin Xu, Lipeng Chang, Yaotian Ding, Fan Li, Zeyu Sun, Lulu Shi, Yuhua Shan, Yaming |
author_sort | Nie, Jiaojiao |
collection | PubMed |
description | Current seasonal influenza vaccines confer only limited coverage of virus strains due to the frequent genetic and antigenic variability of influenza virus (IV). Epitope vaccines that accurately target conserved domains provide a promising approach to increase the breadth of protection; however, poor immunogenicity greatly hinders their application. The protruding (P) domain of the norovirus (NoV), which can self-assemble into a 24-mer particle called the NoV P particle, offers an ideal antigen presentation platform. In this study, a multiepitope nanovaccine displaying influenza epitopes (HMN-PP) was constructed based on the NoV P particle nanoplatform. Large amounts of HMN-PP were easily expressed in Escherichia coli in soluble form. Animal experiments showed that the adjuvanted HMN-PP nanovaccine induced epitope-specific antibodies and haemagglutinin (HA)-specific neutralizing antibodies, and the antibodies could persist for at least three months after the last immunization. Furthermore, HMN-PP induced matrix protein 2 extracellular domain (M2e)-specific antibody-dependent cell-mediated cytotoxicity, CD4(+) and CD8(+) T-cell responses, and a nucleoprotein (NP)-specific cytotoxic T lymphocyte (CTL) response. These results indicated that the combination of a multiepitope vaccine and self-assembled NoV P particles may be an ideal and effective vaccine strategy for highly variable viruses such as IV and SARS-CoV-2. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available in the online version of this article at 10.1007/s12274-023-5395-6. |
format | Online Article Text |
id | pubmed-9933037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Tsinghua University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-99330372023-02-16 Self-assembled multiepitope nanovaccine based on NoV P particles induces effective and lasting protection against H3N2 influenza virus Nie, Jiaojiao Wang, Qingyu Jin, Shenghui Yao, Xin Xu, Lipeng Chang, Yaotian Ding, Fan Li, Zeyu Sun, Lulu Shi, Yuhua Shan, Yaming Nano Res Research Article Current seasonal influenza vaccines confer only limited coverage of virus strains due to the frequent genetic and antigenic variability of influenza virus (IV). Epitope vaccines that accurately target conserved domains provide a promising approach to increase the breadth of protection; however, poor immunogenicity greatly hinders their application. The protruding (P) domain of the norovirus (NoV), which can self-assemble into a 24-mer particle called the NoV P particle, offers an ideal antigen presentation platform. In this study, a multiepitope nanovaccine displaying influenza epitopes (HMN-PP) was constructed based on the NoV P particle nanoplatform. Large amounts of HMN-PP were easily expressed in Escherichia coli in soluble form. Animal experiments showed that the adjuvanted HMN-PP nanovaccine induced epitope-specific antibodies and haemagglutinin (HA)-specific neutralizing antibodies, and the antibodies could persist for at least three months after the last immunization. Furthermore, HMN-PP induced matrix protein 2 extracellular domain (M2e)-specific antibody-dependent cell-mediated cytotoxicity, CD4(+) and CD8(+) T-cell responses, and a nucleoprotein (NP)-specific cytotoxic T lymphocyte (CTL) response. These results indicated that the combination of a multiepitope vaccine and self-assembled NoV P particles may be an ideal and effective vaccine strategy for highly variable viruses such as IV and SARS-CoV-2. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available in the online version of this article at 10.1007/s12274-023-5395-6. Tsinghua University Press 2023-02-16 2023 /pmc/articles/PMC9933037/ /pubmed/36820263 http://dx.doi.org/10.1007/s12274-023-5395-6 Text en © Tsinghua University Press 2023 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Research Article Nie, Jiaojiao Wang, Qingyu Jin, Shenghui Yao, Xin Xu, Lipeng Chang, Yaotian Ding, Fan Li, Zeyu Sun, Lulu Shi, Yuhua Shan, Yaming Self-assembled multiepitope nanovaccine based on NoV P particles induces effective and lasting protection against H3N2 influenza virus |
title | Self-assembled multiepitope nanovaccine based on NoV P particles induces effective and lasting protection against H3N2 influenza virus |
title_full | Self-assembled multiepitope nanovaccine based on NoV P particles induces effective and lasting protection against H3N2 influenza virus |
title_fullStr | Self-assembled multiepitope nanovaccine based on NoV P particles induces effective and lasting protection against H3N2 influenza virus |
title_full_unstemmed | Self-assembled multiepitope nanovaccine based on NoV P particles induces effective and lasting protection against H3N2 influenza virus |
title_short | Self-assembled multiepitope nanovaccine based on NoV P particles induces effective and lasting protection against H3N2 influenza virus |
title_sort | self-assembled multiepitope nanovaccine based on nov p particles induces effective and lasting protection against h3n2 influenza virus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933037/ https://www.ncbi.nlm.nih.gov/pubmed/36820263 http://dx.doi.org/10.1007/s12274-023-5395-6 |
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