Cargando…

Self-assembled multiepitope nanovaccine based on NoV P particles induces effective and lasting protection against H3N2 influenza virus

Current seasonal influenza vaccines confer only limited coverage of virus strains due to the frequent genetic and antigenic variability of influenza virus (IV). Epitope vaccines that accurately target conserved domains provide a promising approach to increase the breadth of protection; however, poor...

Descripción completa

Detalles Bibliográficos
Autores principales: Nie, Jiaojiao, Wang, Qingyu, Jin, Shenghui, Yao, Xin, Xu, Lipeng, Chang, Yaotian, Ding, Fan, Li, Zeyu, Sun, Lulu, Shi, Yuhua, Shan, Yaming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tsinghua University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933037/
https://www.ncbi.nlm.nih.gov/pubmed/36820263
http://dx.doi.org/10.1007/s12274-023-5395-6
_version_ 1784889590249684992
author Nie, Jiaojiao
Wang, Qingyu
Jin, Shenghui
Yao, Xin
Xu, Lipeng
Chang, Yaotian
Ding, Fan
Li, Zeyu
Sun, Lulu
Shi, Yuhua
Shan, Yaming
author_facet Nie, Jiaojiao
Wang, Qingyu
Jin, Shenghui
Yao, Xin
Xu, Lipeng
Chang, Yaotian
Ding, Fan
Li, Zeyu
Sun, Lulu
Shi, Yuhua
Shan, Yaming
author_sort Nie, Jiaojiao
collection PubMed
description Current seasonal influenza vaccines confer only limited coverage of virus strains due to the frequent genetic and antigenic variability of influenza virus (IV). Epitope vaccines that accurately target conserved domains provide a promising approach to increase the breadth of protection; however, poor immunogenicity greatly hinders their application. The protruding (P) domain of the norovirus (NoV), which can self-assemble into a 24-mer particle called the NoV P particle, offers an ideal antigen presentation platform. In this study, a multiepitope nanovaccine displaying influenza epitopes (HMN-PP) was constructed based on the NoV P particle nanoplatform. Large amounts of HMN-PP were easily expressed in Escherichia coli in soluble form. Animal experiments showed that the adjuvanted HMN-PP nanovaccine induced epitope-specific antibodies and haemagglutinin (HA)-specific neutralizing antibodies, and the antibodies could persist for at least three months after the last immunization. Furthermore, HMN-PP induced matrix protein 2 extracellular domain (M2e)-specific antibody-dependent cell-mediated cytotoxicity, CD4(+) and CD8(+) T-cell responses, and a nucleoprotein (NP)-specific cytotoxic T lymphocyte (CTL) response. These results indicated that the combination of a multiepitope vaccine and self-assembled NoV P particles may be an ideal and effective vaccine strategy for highly variable viruses such as IV and SARS-CoV-2. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available in the online version of this article at 10.1007/s12274-023-5395-6.
format Online
Article
Text
id pubmed-9933037
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Tsinghua University Press
record_format MEDLINE/PubMed
spelling pubmed-99330372023-02-16 Self-assembled multiepitope nanovaccine based on NoV P particles induces effective and lasting protection against H3N2 influenza virus Nie, Jiaojiao Wang, Qingyu Jin, Shenghui Yao, Xin Xu, Lipeng Chang, Yaotian Ding, Fan Li, Zeyu Sun, Lulu Shi, Yuhua Shan, Yaming Nano Res Research Article Current seasonal influenza vaccines confer only limited coverage of virus strains due to the frequent genetic and antigenic variability of influenza virus (IV). Epitope vaccines that accurately target conserved domains provide a promising approach to increase the breadth of protection; however, poor immunogenicity greatly hinders their application. The protruding (P) domain of the norovirus (NoV), which can self-assemble into a 24-mer particle called the NoV P particle, offers an ideal antigen presentation platform. In this study, a multiepitope nanovaccine displaying influenza epitopes (HMN-PP) was constructed based on the NoV P particle nanoplatform. Large amounts of HMN-PP were easily expressed in Escherichia coli in soluble form. Animal experiments showed that the adjuvanted HMN-PP nanovaccine induced epitope-specific antibodies and haemagglutinin (HA)-specific neutralizing antibodies, and the antibodies could persist for at least three months after the last immunization. Furthermore, HMN-PP induced matrix protein 2 extracellular domain (M2e)-specific antibody-dependent cell-mediated cytotoxicity, CD4(+) and CD8(+) T-cell responses, and a nucleoprotein (NP)-specific cytotoxic T lymphocyte (CTL) response. These results indicated that the combination of a multiepitope vaccine and self-assembled NoV P particles may be an ideal and effective vaccine strategy for highly variable viruses such as IV and SARS-CoV-2. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available in the online version of this article at 10.1007/s12274-023-5395-6. Tsinghua University Press 2023-02-16 2023 /pmc/articles/PMC9933037/ /pubmed/36820263 http://dx.doi.org/10.1007/s12274-023-5395-6 Text en © Tsinghua University Press 2023 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Research Article
Nie, Jiaojiao
Wang, Qingyu
Jin, Shenghui
Yao, Xin
Xu, Lipeng
Chang, Yaotian
Ding, Fan
Li, Zeyu
Sun, Lulu
Shi, Yuhua
Shan, Yaming
Self-assembled multiepitope nanovaccine based on NoV P particles induces effective and lasting protection against H3N2 influenza virus
title Self-assembled multiepitope nanovaccine based on NoV P particles induces effective and lasting protection against H3N2 influenza virus
title_full Self-assembled multiepitope nanovaccine based on NoV P particles induces effective and lasting protection against H3N2 influenza virus
title_fullStr Self-assembled multiepitope nanovaccine based on NoV P particles induces effective and lasting protection against H3N2 influenza virus
title_full_unstemmed Self-assembled multiepitope nanovaccine based on NoV P particles induces effective and lasting protection against H3N2 influenza virus
title_short Self-assembled multiepitope nanovaccine based on NoV P particles induces effective and lasting protection against H3N2 influenza virus
title_sort self-assembled multiepitope nanovaccine based on nov p particles induces effective and lasting protection against h3n2 influenza virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933037/
https://www.ncbi.nlm.nih.gov/pubmed/36820263
http://dx.doi.org/10.1007/s12274-023-5395-6
work_keys_str_mv AT niejiaojiao selfassembledmultiepitopenanovaccinebasedonnovpparticlesinduceseffectiveandlastingprotectionagainsth3n2influenzavirus
AT wangqingyu selfassembledmultiepitopenanovaccinebasedonnovpparticlesinduceseffectiveandlastingprotectionagainsth3n2influenzavirus
AT jinshenghui selfassembledmultiepitopenanovaccinebasedonnovpparticlesinduceseffectiveandlastingprotectionagainsth3n2influenzavirus
AT yaoxin selfassembledmultiepitopenanovaccinebasedonnovpparticlesinduceseffectiveandlastingprotectionagainsth3n2influenzavirus
AT xulipeng selfassembledmultiepitopenanovaccinebasedonnovpparticlesinduceseffectiveandlastingprotectionagainsth3n2influenzavirus
AT changyaotian selfassembledmultiepitopenanovaccinebasedonnovpparticlesinduceseffectiveandlastingprotectionagainsth3n2influenzavirus
AT dingfan selfassembledmultiepitopenanovaccinebasedonnovpparticlesinduceseffectiveandlastingprotectionagainsth3n2influenzavirus
AT lizeyu selfassembledmultiepitopenanovaccinebasedonnovpparticlesinduceseffectiveandlastingprotectionagainsth3n2influenzavirus
AT sunlulu selfassembledmultiepitopenanovaccinebasedonnovpparticlesinduceseffectiveandlastingprotectionagainsth3n2influenzavirus
AT shiyuhua selfassembledmultiepitopenanovaccinebasedonnovpparticlesinduceseffectiveandlastingprotectionagainsth3n2influenzavirus
AT shanyaming selfassembledmultiepitopenanovaccinebasedonnovpparticlesinduceseffectiveandlastingprotectionagainsth3n2influenzavirus