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A bat MERS-like coronavirus circulates in pangolins and utilizes human DPP4 and host proteases for cell entry
It is unknown whether pangolins, the most trafficked mammals, play a role in the zoonotic transmission of bat coronaviruses. We report the circulation of a novel MERS-like coronavirus in Malayan pangolins, named Manis javanica HKU4-related coronavirus (MjHKU4r-CoV). Among 86 animals, four tested pos...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier Inc.
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933427/ https://www.ncbi.nlm.nih.gov/pubmed/36803605 http://dx.doi.org/10.1016/j.cell.2023.01.019 |
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| author | Chen, Jing Yang, Xinglou Si, Haorui Gong, Qianchun Que, Tengcheng Li, Jing Li, Yang Wu, Chunguang Zhang, Wei Chen, Ying Luo, Yun Zhu, Yan Li, Bei Luo, Dongsheng Hu, Ben Lin, Haofeng Jiang, Rendi Jiang, Tingting Li, Qian Liu, Meiqin Xie, Shizhe Su, Jia Zheng, Xiaoshuang Li, Ang Yao, Yulin Yang, Yong Chen, Panyu Wu, Aiqiong He, Meihong Lin, Xinhua Tong, Yigang Hu, Yanling Shi, Zheng-Li Zhou, Peng |
| author_facet | Chen, Jing Yang, Xinglou Si, Haorui Gong, Qianchun Que, Tengcheng Li, Jing Li, Yang Wu, Chunguang Zhang, Wei Chen, Ying Luo, Yun Zhu, Yan Li, Bei Luo, Dongsheng Hu, Ben Lin, Haofeng Jiang, Rendi Jiang, Tingting Li, Qian Liu, Meiqin Xie, Shizhe Su, Jia Zheng, Xiaoshuang Li, Ang Yao, Yulin Yang, Yong Chen, Panyu Wu, Aiqiong He, Meihong Lin, Xinhua Tong, Yigang Hu, Yanling Shi, Zheng-Li Zhou, Peng |
| author_sort | Chen, Jing |
| collection | PubMed |
| description | It is unknown whether pangolins, the most trafficked mammals, play a role in the zoonotic transmission of bat coronaviruses. We report the circulation of a novel MERS-like coronavirus in Malayan pangolins, named Manis javanica HKU4-related coronavirus (MjHKU4r-CoV). Among 86 animals, four tested positive by pan-CoV PCR, and seven tested seropositive (11 and 12.8%). Four nearly identical (99.9%) genome sequences were obtained, and one virus was isolated (MjHKU4r-CoV-1). This virus utilizes human dipeptidyl peptidase-4 (hDPP4) as a receptor and host proteases for cell infection, which is enhanced by a furin cleavage site that is absent in all known bat HKU4r-CoVs. The MjHKU4r-CoV-1 spike shows higher binding affinity for hDPP4, and MjHKU4r-CoV-1 has a wider host range than bat HKU4-CoV. MjHKU4r-CoV-1 is infectious and pathogenic in human airways and intestinal organs and in hDPP4-transgenic mice. Our study highlights the importance of pangolins as reservoir hosts of coronaviruses poised for human disease emergence. |
| format | Online Article Text |
| id | pubmed-9933427 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | Elsevier Inc. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-99334272023-02-16 A bat MERS-like coronavirus circulates in pangolins and utilizes human DPP4 and host proteases for cell entry Chen, Jing Yang, Xinglou Si, Haorui Gong, Qianchun Que, Tengcheng Li, Jing Li, Yang Wu, Chunguang Zhang, Wei Chen, Ying Luo, Yun Zhu, Yan Li, Bei Luo, Dongsheng Hu, Ben Lin, Haofeng Jiang, Rendi Jiang, Tingting Li, Qian Liu, Meiqin Xie, Shizhe Su, Jia Zheng, Xiaoshuang Li, Ang Yao, Yulin Yang, Yong Chen, Panyu Wu, Aiqiong He, Meihong Lin, Xinhua Tong, Yigang Hu, Yanling Shi, Zheng-Li Zhou, Peng Cell Article It is unknown whether pangolins, the most trafficked mammals, play a role in the zoonotic transmission of bat coronaviruses. We report the circulation of a novel MERS-like coronavirus in Malayan pangolins, named Manis javanica HKU4-related coronavirus (MjHKU4r-CoV). Among 86 animals, four tested positive by pan-CoV PCR, and seven tested seropositive (11 and 12.8%). Four nearly identical (99.9%) genome sequences were obtained, and one virus was isolated (MjHKU4r-CoV-1). This virus utilizes human dipeptidyl peptidase-4 (hDPP4) as a receptor and host proteases for cell infection, which is enhanced by a furin cleavage site that is absent in all known bat HKU4r-CoVs. The MjHKU4r-CoV-1 spike shows higher binding affinity for hDPP4, and MjHKU4r-CoV-1 has a wider host range than bat HKU4-CoV. MjHKU4r-CoV-1 is infectious and pathogenic in human airways and intestinal organs and in hDPP4-transgenic mice. Our study highlights the importance of pangolins as reservoir hosts of coronaviruses poised for human disease emergence. Elsevier Inc. 2023-02-16 2023-02-16 /pmc/articles/PMC9933427/ /pubmed/36803605 http://dx.doi.org/10.1016/j.cell.2023.01.019 Text en © 2023 Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
| spellingShingle | Article Chen, Jing Yang, Xinglou Si, Haorui Gong, Qianchun Que, Tengcheng Li, Jing Li, Yang Wu, Chunguang Zhang, Wei Chen, Ying Luo, Yun Zhu, Yan Li, Bei Luo, Dongsheng Hu, Ben Lin, Haofeng Jiang, Rendi Jiang, Tingting Li, Qian Liu, Meiqin Xie, Shizhe Su, Jia Zheng, Xiaoshuang Li, Ang Yao, Yulin Yang, Yong Chen, Panyu Wu, Aiqiong He, Meihong Lin, Xinhua Tong, Yigang Hu, Yanling Shi, Zheng-Li Zhou, Peng A bat MERS-like coronavirus circulates in pangolins and utilizes human DPP4 and host proteases for cell entry |
| title | A bat MERS-like coronavirus circulates in pangolins and utilizes human DPP4 and host proteases for cell entry |
| title_full | A bat MERS-like coronavirus circulates in pangolins and utilizes human DPP4 and host proteases for cell entry |
| title_fullStr | A bat MERS-like coronavirus circulates in pangolins and utilizes human DPP4 and host proteases for cell entry |
| title_full_unstemmed | A bat MERS-like coronavirus circulates in pangolins and utilizes human DPP4 and host proteases for cell entry |
| title_short | A bat MERS-like coronavirus circulates in pangolins and utilizes human DPP4 and host proteases for cell entry |
| title_sort | bat mers-like coronavirus circulates in pangolins and utilizes human dpp4 and host proteases for cell entry |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933427/ https://www.ncbi.nlm.nih.gov/pubmed/36803605 http://dx.doi.org/10.1016/j.cell.2023.01.019 |
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