An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria

A broad host range (BHR)-inducible T7 RNA polymerase system was developed, enabling induction with isopropyl-β-d-thiogalactopyranoside (IPTG), similar to the Escherichia coli strain BL21(DE3) protocol, but it is now applicable in a wide range of bacteria. This system allows for high protein yields a...

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Detalles Bibliográficos
Autores principales: Greenwich, Jennifer L., Alakavuklar, Melene A., Fuqua, Clay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Other Genetic Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933640/
https://www.ncbi.nlm.nih.gov/pubmed/36645284
http://dx.doi.org/10.1128/mra.01119-22
Descripción
Sumario:A broad host range (BHR)-inducible T7 RNA polymerase system was developed, enabling induction with isopropyl-β-d-thiogalactopyranoside (IPTG), similar to the Escherichia coli strain BL21(DE3) protocol, but it is now applicable in a wide range of bacteria. This system allows for high protein yields and purification from diverse Gram-negative bacteria, including the native host.

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