An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria

A broad host range (BHR)-inducible T7 RNA polymerase system was developed, enabling induction with isopropyl-β-d-thiogalactopyranoside (IPTG), similar to the Escherichia coli strain BL21(DE3) protocol, but it is now applicable in a wide range of bacteria. This system allows for high protein yields a...

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Detalles Bibliográficos
Autores principales: Greenwich, Jennifer L., Alakavuklar, Melene A., Fuqua, Clay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Other Genetic Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933640/
https://www.ncbi.nlm.nih.gov/pubmed/36645284
http://dx.doi.org/10.1128/mra.01119-22
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author Greenwich, Jennifer L.
Alakavuklar, Melene A.
Fuqua, Clay
author_facet Greenwich, Jennifer L.
Alakavuklar, Melene A.
Fuqua, Clay
author_sort Greenwich, Jennifer L.
collection PubMed
description A broad host range (BHR)-inducible T7 RNA polymerase system was developed, enabling induction with isopropyl-β-d-thiogalactopyranoside (IPTG), similar to the Escherichia coli strain BL21(DE3) protocol, but it is now applicable in a wide range of bacteria. This system allows for high protein yields and purification from diverse Gram-negative bacteria, including the native host.
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spelling pubmed-99336402023-02-17 An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria Greenwich, Jennifer L. Alakavuklar, Melene A. Fuqua, Clay Microbiol Resour Announc Other Genetic Resources A broad host range (BHR)-inducible T7 RNA polymerase system was developed, enabling induction with isopropyl-β-d-thiogalactopyranoside (IPTG), similar to the Escherichia coli strain BL21(DE3) protocol, but it is now applicable in a wide range of bacteria. This system allows for high protein yields and purification from diverse Gram-negative bacteria, including the native host. American Society for Microbiology 2023-01-16 /pmc/articles/PMC9933640/ /pubmed/36645284 http://dx.doi.org/10.1128/mra.01119-22 Text en Copyright © 2023 Greenwich et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Other Genetic Resources
Greenwich, Jennifer L.
Alakavuklar, Melene A.
Fuqua, Clay
An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria
title An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria
title_full An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria
title_fullStr An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria
title_full_unstemmed An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria
title_short An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria
title_sort inducible t7 polymerase system for high-level protein expression in diverse gram-negative bacteria
topic Other Genetic Resources
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933640/
https://www.ncbi.nlm.nih.gov/pubmed/36645284
http://dx.doi.org/10.1128/mra.01119-22
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