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An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria
A broad host range (BHR)-inducible T7 RNA polymerase system was developed, enabling induction with isopropyl-β-d-thiogalactopyranoside (IPTG), similar to the Escherichia coli strain BL21(DE3) protocol, but it is now applicable in a wide range of bacteria. This system allows for high protein yields a...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Society for Microbiology
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933640/ https://www.ncbi.nlm.nih.gov/pubmed/36645284 http://dx.doi.org/10.1128/mra.01119-22 |