Cargando…

Frequent Transmission of Streptococcus pneumoniae Serotype 35B and 35D, Clonal Complex 558 Lineage, across Continents and the Formation of Multiple Clades in Japan

Streptococcus pneumoniae is a common bacterial pathogen that causes infections in children worldwide, even after administration of the pneumococcal conjugate vaccine. S. pneumoniae serotype 35B, especially the clonal complex 558 (CC558) lineage, has emerged globally following implementation of the 1...

Descripción completa

Detalles Bibliográficos
Autores principales: Shinohara, Koh, Fujisawa, Takao, Chang, Bin, Ito, Yutaka, Suga, Shigeru, Matsumura, Yasufumi, Yamamoto, Masaki, Nagao, Miki, Ohnishi, Makoto, Sugai, Motoyuki, Nakano, Satoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933736/
https://www.ncbi.nlm.nih.gov/pubmed/36651739
http://dx.doi.org/10.1128/aac.01083-22
_version_ 1784889732364238848
author Shinohara, Koh
Fujisawa, Takao
Chang, Bin
Ito, Yutaka
Suga, Shigeru
Matsumura, Yasufumi
Yamamoto, Masaki
Nagao, Miki
Ohnishi, Makoto
Sugai, Motoyuki
Nakano, Satoshi
author_facet Shinohara, Koh
Fujisawa, Takao
Chang, Bin
Ito, Yutaka
Suga, Shigeru
Matsumura, Yasufumi
Yamamoto, Masaki
Nagao, Miki
Ohnishi, Makoto
Sugai, Motoyuki
Nakano, Satoshi
author_sort Shinohara, Koh
collection PubMed
description Streptococcus pneumoniae is a common bacterial pathogen that causes infections in children worldwide, even after administration of the pneumococcal conjugate vaccine. S. pneumoniae serotype 35B, especially the clonal complex 558 (CC558) lineage, has emerged globally following implementation of the 13-valent pneumococcal conjugate vaccine. Serotype 35B strains are also associated with multidrug resistance to both β-lactams and non-β-lactam drugs. In addition, a novel serotype, 35D, which is closely related to 35B and differs in polysaccharide structure, was recently reported. However, the genetic relationship among globally disseminating serotype 35B and D (35B/D) strains remains unknown. To investigate the molecular epidemiology of global serotype 35B/D strains, we conducted a genomic analysis of serotype 35B/D strains from various continents, including those from the Japanese national surveillance collection. A total of 87 isolates were identified as serotype 35B/D in the Japanese surveillance collection (n = 1,358). All the isolates were assigned to either CC558 or CC2755. Serotype 35D isolates were interspersed with serotype 35B isolates. Phylogenetic analysis revealed the formation of multiple clusters by the Japanese serotype 35B/D-CC558 isolates among the foreign isolates, which suggested multiple events of introduction of the clone into Japan. The global 35B/D-CC558 strains were found to share specific penicillin-binding protein profiles, pbp1a-4, pbp2b-7, and pbp2x-7, associated with penicillin, cephalosporin, and carbapenem nonsusceptibility. Moreover, 88.5% of the Japanese 35B/D-CC558 and 35B/D-CC2755 isolates were found to harbor the Tn916-like integrative and conjugative elements Tn2009, Tn2010, and Tn6002, associated with multidrug resistance to macrolides and tetracyclines. The results of this study imply that serotype 35B/D-CC558 strains could be frequently transmitted intercontinentally.
format Online
Article
Text
id pubmed-9933736
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-99337362023-02-17 Frequent Transmission of Streptococcus pneumoniae Serotype 35B and 35D, Clonal Complex 558 Lineage, across Continents and the Formation of Multiple Clades in Japan Shinohara, Koh Fujisawa, Takao Chang, Bin Ito, Yutaka Suga, Shigeru Matsumura, Yasufumi Yamamoto, Masaki Nagao, Miki Ohnishi, Makoto Sugai, Motoyuki Nakano, Satoshi Antimicrob Agents Chemother Epidemiology and Surveillance Streptococcus pneumoniae is a common bacterial pathogen that causes infections in children worldwide, even after administration of the pneumococcal conjugate vaccine. S. pneumoniae serotype 35B, especially the clonal complex 558 (CC558) lineage, has emerged globally following implementation of the 13-valent pneumococcal conjugate vaccine. Serotype 35B strains are also associated with multidrug resistance to both β-lactams and non-β-lactam drugs. In addition, a novel serotype, 35D, which is closely related to 35B and differs in polysaccharide structure, was recently reported. However, the genetic relationship among globally disseminating serotype 35B and D (35B/D) strains remains unknown. To investigate the molecular epidemiology of global serotype 35B/D strains, we conducted a genomic analysis of serotype 35B/D strains from various continents, including those from the Japanese national surveillance collection. A total of 87 isolates were identified as serotype 35B/D in the Japanese surveillance collection (n = 1,358). All the isolates were assigned to either CC558 or CC2755. Serotype 35D isolates were interspersed with serotype 35B isolates. Phylogenetic analysis revealed the formation of multiple clusters by the Japanese serotype 35B/D-CC558 isolates among the foreign isolates, which suggested multiple events of introduction of the clone into Japan. The global 35B/D-CC558 strains were found to share specific penicillin-binding protein profiles, pbp1a-4, pbp2b-7, and pbp2x-7, associated with penicillin, cephalosporin, and carbapenem nonsusceptibility. Moreover, 88.5% of the Japanese 35B/D-CC558 and 35B/D-CC2755 isolates were found to harbor the Tn916-like integrative and conjugative elements Tn2009, Tn2010, and Tn6002, associated with multidrug resistance to macrolides and tetracyclines. The results of this study imply that serotype 35B/D-CC558 strains could be frequently transmitted intercontinentally. American Society for Microbiology 2023-01-18 /pmc/articles/PMC9933736/ /pubmed/36651739 http://dx.doi.org/10.1128/aac.01083-22 Text en Copyright © 2023 Shinohara et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Epidemiology and Surveillance
Shinohara, Koh
Fujisawa, Takao
Chang, Bin
Ito, Yutaka
Suga, Shigeru
Matsumura, Yasufumi
Yamamoto, Masaki
Nagao, Miki
Ohnishi, Makoto
Sugai, Motoyuki
Nakano, Satoshi
Frequent Transmission of Streptococcus pneumoniae Serotype 35B and 35D, Clonal Complex 558 Lineage, across Continents and the Formation of Multiple Clades in Japan
title Frequent Transmission of Streptococcus pneumoniae Serotype 35B and 35D, Clonal Complex 558 Lineage, across Continents and the Formation of Multiple Clades in Japan
title_full Frequent Transmission of Streptococcus pneumoniae Serotype 35B and 35D, Clonal Complex 558 Lineage, across Continents and the Formation of Multiple Clades in Japan
title_fullStr Frequent Transmission of Streptococcus pneumoniae Serotype 35B and 35D, Clonal Complex 558 Lineage, across Continents and the Formation of Multiple Clades in Japan
title_full_unstemmed Frequent Transmission of Streptococcus pneumoniae Serotype 35B and 35D, Clonal Complex 558 Lineage, across Continents and the Formation of Multiple Clades in Japan
title_short Frequent Transmission of Streptococcus pneumoniae Serotype 35B and 35D, Clonal Complex 558 Lineage, across Continents and the Formation of Multiple Clades in Japan
title_sort frequent transmission of streptococcus pneumoniae serotype 35b and 35d, clonal complex 558 lineage, across continents and the formation of multiple clades in japan
topic Epidemiology and Surveillance
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933736/
https://www.ncbi.nlm.nih.gov/pubmed/36651739
http://dx.doi.org/10.1128/aac.01083-22
work_keys_str_mv AT shinoharakoh frequenttransmissionofstreptococcuspneumoniaeserotype35band35dclonalcomplex558lineageacrosscontinentsandtheformationofmultiplecladesinjapan
AT fujisawatakao frequenttransmissionofstreptococcuspneumoniaeserotype35band35dclonalcomplex558lineageacrosscontinentsandtheformationofmultiplecladesinjapan
AT changbin frequenttransmissionofstreptococcuspneumoniaeserotype35band35dclonalcomplex558lineageacrosscontinentsandtheformationofmultiplecladesinjapan
AT itoyutaka frequenttransmissionofstreptococcuspneumoniaeserotype35band35dclonalcomplex558lineageacrosscontinentsandtheformationofmultiplecladesinjapan
AT sugashigeru frequenttransmissionofstreptococcuspneumoniaeserotype35band35dclonalcomplex558lineageacrosscontinentsandtheformationofmultiplecladesinjapan
AT matsumurayasufumi frequenttransmissionofstreptococcuspneumoniaeserotype35band35dclonalcomplex558lineageacrosscontinentsandtheformationofmultiplecladesinjapan
AT yamamotomasaki frequenttransmissionofstreptococcuspneumoniaeserotype35band35dclonalcomplex558lineageacrosscontinentsandtheformationofmultiplecladesinjapan
AT nagaomiki frequenttransmissionofstreptococcuspneumoniaeserotype35band35dclonalcomplex558lineageacrosscontinentsandtheformationofmultiplecladesinjapan
AT ohnishimakoto frequenttransmissionofstreptococcuspneumoniaeserotype35band35dclonalcomplex558lineageacrosscontinentsandtheformationofmultiplecladesinjapan
AT sugaimotoyuki frequenttransmissionofstreptococcuspneumoniaeserotype35band35dclonalcomplex558lineageacrosscontinentsandtheformationofmultiplecladesinjapan
AT nakanosatoshi frequenttransmissionofstreptococcuspneumoniaeserotype35band35dclonalcomplex558lineageacrosscontinentsandtheformationofmultiplecladesinjapan