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Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 gene
Coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), encode a proofreading exonuclease, nonstructural protein 14 (nsp14), that helps ensure replication competence at a low evolutionary rate compared with other RNA viruses. In the current pandemic, SARS-CoV-2 has acc...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933857/ https://www.ncbi.nlm.nih.gov/pubmed/36811085 http://dx.doi.org/10.1016/j.isci.2023.106210 |
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author | Takada, Kosuke Ueda, Mahoko Takahashi Shichinohe, Shintaro Kida, Yurie Ono, Chikako Matsuura, Yoshiharu Watanabe, Tokiko Nakagawa, So |
author_facet | Takada, Kosuke Ueda, Mahoko Takahashi Shichinohe, Shintaro Kida, Yurie Ono, Chikako Matsuura, Yoshiharu Watanabe, Tokiko Nakagawa, So |
author_sort | Takada, Kosuke |
collection | PubMed |
description | Coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), encode a proofreading exonuclease, nonstructural protein 14 (nsp14), that helps ensure replication competence at a low evolutionary rate compared with other RNA viruses. In the current pandemic, SARS-CoV-2 has accumulated diverse genomic mutations including in nsp14. Here, to clarify whether amino acid substitutions in nsp14 affect the genomic diversity and evolution of SARS-CoV-2, we searched for amino acid substitutions in nature that may interfere with nsp14 function. We found that viruses carrying a proline-to-leucine change at position 203 (P203L) have a high evolutionary rate and that a recombinant SARS-CoV-2 virus with the P203L mutation acquired more diverse genomic mutations than wild-type virus during its replication in hamsters. Our findings suggest that substitutions, such as P203L, in nsp14 may accelerate the genomic diversity of SARS-CoV-2, contributing to virus evolution during the pandemic. |
format | Online Article Text |
id | pubmed-9933857 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-99338572023-02-17 Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 gene Takada, Kosuke Ueda, Mahoko Takahashi Shichinohe, Shintaro Kida, Yurie Ono, Chikako Matsuura, Yoshiharu Watanabe, Tokiko Nakagawa, So iScience Article Coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), encode a proofreading exonuclease, nonstructural protein 14 (nsp14), that helps ensure replication competence at a low evolutionary rate compared with other RNA viruses. In the current pandemic, SARS-CoV-2 has accumulated diverse genomic mutations including in nsp14. Here, to clarify whether amino acid substitutions in nsp14 affect the genomic diversity and evolution of SARS-CoV-2, we searched for amino acid substitutions in nature that may interfere with nsp14 function. We found that viruses carrying a proline-to-leucine change at position 203 (P203L) have a high evolutionary rate and that a recombinant SARS-CoV-2 virus with the P203L mutation acquired more diverse genomic mutations than wild-type virus during its replication in hamsters. Our findings suggest that substitutions, such as P203L, in nsp14 may accelerate the genomic diversity of SARS-CoV-2, contributing to virus evolution during the pandemic. Elsevier 2023-02-16 /pmc/articles/PMC9933857/ /pubmed/36811085 http://dx.doi.org/10.1016/j.isci.2023.106210 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Takada, Kosuke Ueda, Mahoko Takahashi Shichinohe, Shintaro Kida, Yurie Ono, Chikako Matsuura, Yoshiharu Watanabe, Tokiko Nakagawa, So Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 gene |
title | Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 gene |
title_full | Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 gene |
title_fullStr | Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 gene |
title_full_unstemmed | Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 gene |
title_short | Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 gene |
title_sort | genomic diversity of sars-cov-2 can be accelerated by mutations in the nsp14 gene |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933857/ https://www.ncbi.nlm.nih.gov/pubmed/36811085 http://dx.doi.org/10.1016/j.isci.2023.106210 |
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