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Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 gene

Coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), encode a proofreading exonuclease, nonstructural protein 14 (nsp14), that helps ensure replication competence at a low evolutionary rate compared with other RNA viruses. In the current pandemic, SARS-CoV-2 has acc...

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Autores principales: Takada, Kosuke, Ueda, Mahoko Takahashi, Shichinohe, Shintaro, Kida, Yurie, Ono, Chikako, Matsuura, Yoshiharu, Watanabe, Tokiko, Nakagawa, So
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933857/
https://www.ncbi.nlm.nih.gov/pubmed/36811085
http://dx.doi.org/10.1016/j.isci.2023.106210
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author Takada, Kosuke
Ueda, Mahoko Takahashi
Shichinohe, Shintaro
Kida, Yurie
Ono, Chikako
Matsuura, Yoshiharu
Watanabe, Tokiko
Nakagawa, So
author_facet Takada, Kosuke
Ueda, Mahoko Takahashi
Shichinohe, Shintaro
Kida, Yurie
Ono, Chikako
Matsuura, Yoshiharu
Watanabe, Tokiko
Nakagawa, So
author_sort Takada, Kosuke
collection PubMed
description Coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), encode a proofreading exonuclease, nonstructural protein 14 (nsp14), that helps ensure replication competence at a low evolutionary rate compared with other RNA viruses. In the current pandemic, SARS-CoV-2 has accumulated diverse genomic mutations including in nsp14. Here, to clarify whether amino acid substitutions in nsp14 affect the genomic diversity and evolution of SARS-CoV-2, we searched for amino acid substitutions in nature that may interfere with nsp14 function. We found that viruses carrying a proline-to-leucine change at position 203 (P203L) have a high evolutionary rate and that a recombinant SARS-CoV-2 virus with the P203L mutation acquired more diverse genomic mutations than wild-type virus during its replication in hamsters. Our findings suggest that substitutions, such as P203L, in nsp14 may accelerate the genomic diversity of SARS-CoV-2, contributing to virus evolution during the pandemic.
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spelling pubmed-99338572023-02-17 Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 gene Takada, Kosuke Ueda, Mahoko Takahashi Shichinohe, Shintaro Kida, Yurie Ono, Chikako Matsuura, Yoshiharu Watanabe, Tokiko Nakagawa, So iScience Article Coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), encode a proofreading exonuclease, nonstructural protein 14 (nsp14), that helps ensure replication competence at a low evolutionary rate compared with other RNA viruses. In the current pandemic, SARS-CoV-2 has accumulated diverse genomic mutations including in nsp14. Here, to clarify whether amino acid substitutions in nsp14 affect the genomic diversity and evolution of SARS-CoV-2, we searched for amino acid substitutions in nature that may interfere with nsp14 function. We found that viruses carrying a proline-to-leucine change at position 203 (P203L) have a high evolutionary rate and that a recombinant SARS-CoV-2 virus with the P203L mutation acquired more diverse genomic mutations than wild-type virus during its replication in hamsters. Our findings suggest that substitutions, such as P203L, in nsp14 may accelerate the genomic diversity of SARS-CoV-2, contributing to virus evolution during the pandemic. Elsevier 2023-02-16 /pmc/articles/PMC9933857/ /pubmed/36811085 http://dx.doi.org/10.1016/j.isci.2023.106210 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Takada, Kosuke
Ueda, Mahoko Takahashi
Shichinohe, Shintaro
Kida, Yurie
Ono, Chikako
Matsuura, Yoshiharu
Watanabe, Tokiko
Nakagawa, So
Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 gene
title Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 gene
title_full Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 gene
title_fullStr Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 gene
title_full_unstemmed Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 gene
title_short Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 gene
title_sort genomic diversity of sars-cov-2 can be accelerated by mutations in the nsp14 gene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933857/
https://www.ncbi.nlm.nih.gov/pubmed/36811085
http://dx.doi.org/10.1016/j.isci.2023.106210
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