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Hepatitis B virus genotypes and drug resistance mutations circulating in blood donors in Beira, Mozambique

Hepatitis B virus (HBV) infects nearly 300 million people and is the leading cause of hepatitis and hepatocellular carcinoma worldwide. Despite the high burden of HBV in sub-Saharan Africa, countries such as Mozambique have limited data available on circulating HBV genotypes and the presence of drug...

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Autores principales: Mathew, Ann, Ismael, Nalia, Meeds, Heidi, Vubil, Adolfo, Zicai, Ana Flora, Mabunda, Nédio, Blackard, Jason T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9934330/
https://www.ncbi.nlm.nih.gov/pubmed/36795797
http://dx.doi.org/10.1371/journal.pone.0281855
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author Mathew, Ann
Ismael, Nalia
Meeds, Heidi
Vubil, Adolfo
Zicai, Ana Flora
Mabunda, Nédio
Blackard, Jason T.
author_facet Mathew, Ann
Ismael, Nalia
Meeds, Heidi
Vubil, Adolfo
Zicai, Ana Flora
Mabunda, Nédio
Blackard, Jason T.
author_sort Mathew, Ann
collection PubMed
description Hepatitis B virus (HBV) infects nearly 300 million people and is the leading cause of hepatitis and hepatocellular carcinoma worldwide. Despite the high burden of HBV in sub-Saharan Africa, countries such as Mozambique have limited data available on circulating HBV genotypes and the presence of drug resistance mutations. Blood donors from Beira, Mozambique were tested for HBV surface antigen (HBsAg) and HBV DNA at the Instituto Nacional de Saúde in Maputo, Mozambique. Regardless of HBsAg status, donors with detectable HBV DNA were evaluated for HBV genotype. PCR was performed with primers amplifying a 2.1–2.2 kilobase fragment of the HBV genome. PCR products were submitted for next generation sequencing (NGS), and consensus sequences were evaluated for HBV genotype, recombination, and the presence or absence of drug resistance mutations. Of the 1281 blood donors tested, 74 had quantifiable HBV DNA. The polymerase gene could be amplified from 45 of 58 (77.6%) individuals with chronic HBV infection and 12 of 16 (75%) with occult HBV infection. Among these 57, 51 (89.5%) sequences belonged to HBV genotype A1, while 6 (10.5%) were HBV genotype E. All genotype E sequences were E/A recombinants, and clustered separately from other genotype E references. Genotype A samples had a median viral load of 637 IU/mL, while genotype E samples had a median viral load of 476,084 IU/mL. No drug resistance mutations were observed in the consensus sequences. The current study demonstrates the genotypic diversity of HBV in blood donors in Mozambique, but the absence of dominant (consensus) drug resistance mutations. Studies in other at-risk populations are essential for understanding the epidemiology, risk of liver disease, and likelihood of treatment resistance in resource-limited settings.
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spelling pubmed-99343302023-02-17 Hepatitis B virus genotypes and drug resistance mutations circulating in blood donors in Beira, Mozambique Mathew, Ann Ismael, Nalia Meeds, Heidi Vubil, Adolfo Zicai, Ana Flora Mabunda, Nédio Blackard, Jason T. PLoS One Research Article Hepatitis B virus (HBV) infects nearly 300 million people and is the leading cause of hepatitis and hepatocellular carcinoma worldwide. Despite the high burden of HBV in sub-Saharan Africa, countries such as Mozambique have limited data available on circulating HBV genotypes and the presence of drug resistance mutations. Blood donors from Beira, Mozambique were tested for HBV surface antigen (HBsAg) and HBV DNA at the Instituto Nacional de Saúde in Maputo, Mozambique. Regardless of HBsAg status, donors with detectable HBV DNA were evaluated for HBV genotype. PCR was performed with primers amplifying a 2.1–2.2 kilobase fragment of the HBV genome. PCR products were submitted for next generation sequencing (NGS), and consensus sequences were evaluated for HBV genotype, recombination, and the presence or absence of drug resistance mutations. Of the 1281 blood donors tested, 74 had quantifiable HBV DNA. The polymerase gene could be amplified from 45 of 58 (77.6%) individuals with chronic HBV infection and 12 of 16 (75%) with occult HBV infection. Among these 57, 51 (89.5%) sequences belonged to HBV genotype A1, while 6 (10.5%) were HBV genotype E. All genotype E sequences were E/A recombinants, and clustered separately from other genotype E references. Genotype A samples had a median viral load of 637 IU/mL, while genotype E samples had a median viral load of 476,084 IU/mL. No drug resistance mutations were observed in the consensus sequences. The current study demonstrates the genotypic diversity of HBV in blood donors in Mozambique, but the absence of dominant (consensus) drug resistance mutations. Studies in other at-risk populations are essential for understanding the epidemiology, risk of liver disease, and likelihood of treatment resistance in resource-limited settings. Public Library of Science 2023-02-16 /pmc/articles/PMC9934330/ /pubmed/36795797 http://dx.doi.org/10.1371/journal.pone.0281855 Text en © 2023 Mathew et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Mathew, Ann
Ismael, Nalia
Meeds, Heidi
Vubil, Adolfo
Zicai, Ana Flora
Mabunda, Nédio
Blackard, Jason T.
Hepatitis B virus genotypes and drug resistance mutations circulating in blood donors in Beira, Mozambique
title Hepatitis B virus genotypes and drug resistance mutations circulating in blood donors in Beira, Mozambique
title_full Hepatitis B virus genotypes and drug resistance mutations circulating in blood donors in Beira, Mozambique
title_fullStr Hepatitis B virus genotypes and drug resistance mutations circulating in blood donors in Beira, Mozambique
title_full_unstemmed Hepatitis B virus genotypes and drug resistance mutations circulating in blood donors in Beira, Mozambique
title_short Hepatitis B virus genotypes and drug resistance mutations circulating in blood donors in Beira, Mozambique
title_sort hepatitis b virus genotypes and drug resistance mutations circulating in blood donors in beira, mozambique
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9934330/
https://www.ncbi.nlm.nih.gov/pubmed/36795797
http://dx.doi.org/10.1371/journal.pone.0281855
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