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Comparison of human amniotic membrane and collagen nerve wraps around sciatic nerve reverse autografts in a rat model()

Human amniotic membrane (hAM) and collagen nerve wraps are biomaterials that have been investigated as therapies for improving outcomes of peripheral nerve regeneration; however, their efficacy has not been compared. The purpose of this study is to compare the efficacy of collagen and human amniotic...

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Autores principales: Wolfe, Erin M., Mathis, Sydney A., de la Olivo Muñoz, Natalia, Ovadia, Steven A., Panthaki, Zubin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9934491/
https://www.ncbi.nlm.nih.gov/pubmed/36824162
http://dx.doi.org/10.1016/j.bbiosy.2022.100048
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author Wolfe, Erin M.
Mathis, Sydney A.
de la Olivo Muñoz, Natalia
Ovadia, Steven A.
Panthaki, Zubin J.
author_facet Wolfe, Erin M.
Mathis, Sydney A.
de la Olivo Muñoz, Natalia
Ovadia, Steven A.
Panthaki, Zubin J.
author_sort Wolfe, Erin M.
collection PubMed
description Human amniotic membrane (hAM) and collagen nerve wraps are biomaterials that have been investigated as therapies for improving outcomes of peripheral nerve regeneration; however, their efficacy has not been compared. The purpose of this study is to compare the efficacy of collagen and human amniotic membrane nerve wraps in a rodent sciatic nerve reverse autograft model. Lewis rats (n = 29) underwent sciatic nerve injury and repair in which a 10-mm gap was bridged with reverse autograft combined with either no nerve wrap (control), collagen nerve wrap or hAM nerve wrap. Behavioral analyses were performed at baseline and 4, 8 and 12 weeks. Electrophysiological studies were conducted at 8, 10 and 12 weeks. Additional outcomes assessed included gastrocnemius muscle weights, nerve adhesions, axonal regeneration and scarring at 12 weeks. Application of both collagen and hAM nerve wraps resulted in improvement of functional and histologic outcomes when compared with controls, with a greater magnitude of improvement for the experimental group treated with hAM nerve wraps. hAM-treated animals had significantly higher numbers of axons compared to control animals (p < 0.05) and significantly less perineural fibrosis than both control and collagen treated nerves (p < 0.05). The ratio of experimental to control gastrocnemius weights was significantly greater in hAM compared to control samples (p < 0.05). We conclude that hAM nerve wraps are a promising biomaterial that is effective for improving outcomes of peripheral nerve regeneration, resulting in superior nerve regeneration and functional recovery compared to collagen nerve wraps and controls.
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spelling pubmed-99344912023-02-22 Comparison of human amniotic membrane and collagen nerve wraps around sciatic nerve reverse autografts in a rat model() Wolfe, Erin M. Mathis, Sydney A. de la Olivo Muñoz, Natalia Ovadia, Steven A. Panthaki, Zubin J. Biomater Biosyst Research Article Human amniotic membrane (hAM) and collagen nerve wraps are biomaterials that have been investigated as therapies for improving outcomes of peripheral nerve regeneration; however, their efficacy has not been compared. The purpose of this study is to compare the efficacy of collagen and human amniotic membrane nerve wraps in a rodent sciatic nerve reverse autograft model. Lewis rats (n = 29) underwent sciatic nerve injury and repair in which a 10-mm gap was bridged with reverse autograft combined with either no nerve wrap (control), collagen nerve wrap or hAM nerve wrap. Behavioral analyses were performed at baseline and 4, 8 and 12 weeks. Electrophysiological studies were conducted at 8, 10 and 12 weeks. Additional outcomes assessed included gastrocnemius muscle weights, nerve adhesions, axonal regeneration and scarring at 12 weeks. Application of both collagen and hAM nerve wraps resulted in improvement of functional and histologic outcomes when compared with controls, with a greater magnitude of improvement for the experimental group treated with hAM nerve wraps. hAM-treated animals had significantly higher numbers of axons compared to control animals (p < 0.05) and significantly less perineural fibrosis than both control and collagen treated nerves (p < 0.05). The ratio of experimental to control gastrocnemius weights was significantly greater in hAM compared to control samples (p < 0.05). We conclude that hAM nerve wraps are a promising biomaterial that is effective for improving outcomes of peripheral nerve regeneration, resulting in superior nerve regeneration and functional recovery compared to collagen nerve wraps and controls. Elsevier 2022-04-07 /pmc/articles/PMC9934491/ /pubmed/36824162 http://dx.doi.org/10.1016/j.bbiosy.2022.100048 Text en © 2022 The Authors. Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Wolfe, Erin M.
Mathis, Sydney A.
de la Olivo Muñoz, Natalia
Ovadia, Steven A.
Panthaki, Zubin J.
Comparison of human amniotic membrane and collagen nerve wraps around sciatic nerve reverse autografts in a rat model()
title Comparison of human amniotic membrane and collagen nerve wraps around sciatic nerve reverse autografts in a rat model()
title_full Comparison of human amniotic membrane and collagen nerve wraps around sciatic nerve reverse autografts in a rat model()
title_fullStr Comparison of human amniotic membrane and collagen nerve wraps around sciatic nerve reverse autografts in a rat model()
title_full_unstemmed Comparison of human amniotic membrane and collagen nerve wraps around sciatic nerve reverse autografts in a rat model()
title_short Comparison of human amniotic membrane and collagen nerve wraps around sciatic nerve reverse autografts in a rat model()
title_sort comparison of human amniotic membrane and collagen nerve wraps around sciatic nerve reverse autografts in a rat model()
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9934491/
https://www.ncbi.nlm.nih.gov/pubmed/36824162
http://dx.doi.org/10.1016/j.bbiosy.2022.100048
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