The tetraspanin TSPAN5 regulates AMPAR exocytosis by interacting with the AP4 complex

Intracellular trafficking of AMPA receptors is a tightly regulated process which involves several adaptor proteins, and is crucial for the activity of excitatory synapses both in basal conditions and during synaptic plasticity. We found that, in rat hippocampal neurons, an intracellular pool of the...

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Autores principales: Moretto, Edoardo, Miozzo, Federico, Longatti, Anna, Bonnet, Caroline, Coussen, Francoise, Jaudon, Fanny, Cingolani, Lorenzo A, Passafaro, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9934860/
https://www.ncbi.nlm.nih.gov/pubmed/36795458
http://dx.doi.org/10.7554/eLife.76425
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author Moretto, Edoardo
Miozzo, Federico
Longatti, Anna
Bonnet, Caroline
Coussen, Francoise
Jaudon, Fanny
Cingolani, Lorenzo A
Passafaro, Maria
author_facet Moretto, Edoardo
Miozzo, Federico
Longatti, Anna
Bonnet, Caroline
Coussen, Francoise
Jaudon, Fanny
Cingolani, Lorenzo A
Passafaro, Maria
author_sort Moretto, Edoardo
collection PubMed
description Intracellular trafficking of AMPA receptors is a tightly regulated process which involves several adaptor proteins, and is crucial for the activity of excitatory synapses both in basal conditions and during synaptic plasticity. We found that, in rat hippocampal neurons, an intracellular pool of the tetraspanin TSPAN5 promotes exocytosis of AMPA receptors without affecting their internalisation. TSPAN5 mediates this function by interacting with the adaptor protein complex AP4 and Stargazin and possibly using recycling endosomes as a delivery route. This work highlights TSPAN5 as a new adaptor regulating AMPA receptor trafficking.
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spelling pubmed-99348602023-02-17 The tetraspanin TSPAN5 regulates AMPAR exocytosis by interacting with the AP4 complex Moretto, Edoardo Miozzo, Federico Longatti, Anna Bonnet, Caroline Coussen, Francoise Jaudon, Fanny Cingolani, Lorenzo A Passafaro, Maria eLife Neuroscience Intracellular trafficking of AMPA receptors is a tightly regulated process which involves several adaptor proteins, and is crucial for the activity of excitatory synapses both in basal conditions and during synaptic plasticity. We found that, in rat hippocampal neurons, an intracellular pool of the tetraspanin TSPAN5 promotes exocytosis of AMPA receptors without affecting their internalisation. TSPAN5 mediates this function by interacting with the adaptor protein complex AP4 and Stargazin and possibly using recycling endosomes as a delivery route. This work highlights TSPAN5 as a new adaptor regulating AMPA receptor trafficking. eLife Sciences Publications, Ltd 2023-02-16 /pmc/articles/PMC9934860/ /pubmed/36795458 http://dx.doi.org/10.7554/eLife.76425 Text en © 2023, Moretto et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Moretto, Edoardo
Miozzo, Federico
Longatti, Anna
Bonnet, Caroline
Coussen, Francoise
Jaudon, Fanny
Cingolani, Lorenzo A
Passafaro, Maria
The tetraspanin TSPAN5 regulates AMPAR exocytosis by interacting with the AP4 complex
title The tetraspanin TSPAN5 regulates AMPAR exocytosis by interacting with the AP4 complex
title_full The tetraspanin TSPAN5 regulates AMPAR exocytosis by interacting with the AP4 complex
title_fullStr The tetraspanin TSPAN5 regulates AMPAR exocytosis by interacting with the AP4 complex
title_full_unstemmed The tetraspanin TSPAN5 regulates AMPAR exocytosis by interacting with the AP4 complex
title_short The tetraspanin TSPAN5 regulates AMPAR exocytosis by interacting with the AP4 complex
title_sort tetraspanin tspan5 regulates ampar exocytosis by interacting with the ap4 complex
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9934860/
https://www.ncbi.nlm.nih.gov/pubmed/36795458
http://dx.doi.org/10.7554/eLife.76425
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