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Structures of transcription preinitiation complex engaged with the +1 nucleosome

The preinitiation complex (PIC) assembles on promoters of protein-coding genes to position RNA polymerase II (Pol II) for transcription initiation. Previous structural studies revealed the PIC on different promoters, but did not address how the PIC assembles within chromatin. In the yeast Saccharomy...

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Autores principales: Wang, Haibo, Schilbach, Sandra, Ninov, Momchil, Urlaub, Henning, Cramer, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9935396/
https://www.ncbi.nlm.nih.gov/pubmed/36411341
http://dx.doi.org/10.1038/s41594-022-00865-w
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author Wang, Haibo
Schilbach, Sandra
Ninov, Momchil
Urlaub, Henning
Cramer, Patrick
author_facet Wang, Haibo
Schilbach, Sandra
Ninov, Momchil
Urlaub, Henning
Cramer, Patrick
author_sort Wang, Haibo
collection PubMed
description The preinitiation complex (PIC) assembles on promoters of protein-coding genes to position RNA polymerase II (Pol II) for transcription initiation. Previous structural studies revealed the PIC on different promoters, but did not address how the PIC assembles within chromatin. In the yeast Saccharomyces cerevisiae, PIC assembly occurs adjacent to the +1 nucleosome that is located downstream of the core promoter. Here we present cryo-EM structures of the yeast PIC bound to promoter DNA and the +1 nucleosome located at three different positions. The general transcription factor TFIIH engages with the incoming downstream nucleosome and its translocase subunit Ssl2 (XPB in human TFIIH) drives the rotation of the +1 nucleosome leading to partial detachment of nucleosomal DNA and intimate interactions between TFIIH and the nucleosome. The structures provide insights into how transcription initiation can be influenced by the +1 nucleosome and may explain why the transcription start site is often located roughly 60 base pairs upstream of the dyad of the +1 nucleosome in yeast.
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spelling pubmed-99353962023-02-18 Structures of transcription preinitiation complex engaged with the +1 nucleosome Wang, Haibo Schilbach, Sandra Ninov, Momchil Urlaub, Henning Cramer, Patrick Nat Struct Mol Biol Article The preinitiation complex (PIC) assembles on promoters of protein-coding genes to position RNA polymerase II (Pol II) for transcription initiation. Previous structural studies revealed the PIC on different promoters, but did not address how the PIC assembles within chromatin. In the yeast Saccharomyces cerevisiae, PIC assembly occurs adjacent to the +1 nucleosome that is located downstream of the core promoter. Here we present cryo-EM structures of the yeast PIC bound to promoter DNA and the +1 nucleosome located at three different positions. The general transcription factor TFIIH engages with the incoming downstream nucleosome and its translocase subunit Ssl2 (XPB in human TFIIH) drives the rotation of the +1 nucleosome leading to partial detachment of nucleosomal DNA and intimate interactions between TFIIH and the nucleosome. The structures provide insights into how transcription initiation can be influenced by the +1 nucleosome and may explain why the transcription start site is often located roughly 60 base pairs upstream of the dyad of the +1 nucleosome in yeast. Nature Publishing Group US 2022-11-21 2023 /pmc/articles/PMC9935396/ /pubmed/36411341 http://dx.doi.org/10.1038/s41594-022-00865-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wang, Haibo
Schilbach, Sandra
Ninov, Momchil
Urlaub, Henning
Cramer, Patrick
Structures of transcription preinitiation complex engaged with the +1 nucleosome
title Structures of transcription preinitiation complex engaged with the +1 nucleosome
title_full Structures of transcription preinitiation complex engaged with the +1 nucleosome
title_fullStr Structures of transcription preinitiation complex engaged with the +1 nucleosome
title_full_unstemmed Structures of transcription preinitiation complex engaged with the +1 nucleosome
title_short Structures of transcription preinitiation complex engaged with the +1 nucleosome
title_sort structures of transcription preinitiation complex engaged with the +1 nucleosome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9935396/
https://www.ncbi.nlm.nih.gov/pubmed/36411341
http://dx.doi.org/10.1038/s41594-022-00865-w
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