Cargando…

Visualizing orthogonal RNAs simultaneously in live mammalian cells by fluorescence lifetime imaging microscopy (FLIM)

Visualization of RNAs in live cells is critical to understand biology of RNA dynamics and function in the complex cellular environment. Detection of RNAs with a fluorescent marker frequently involves genetically fusing an RNA aptamer tag to the RNA of interest, which binds to small molecules that ar...

Descripción completa

Detalles Bibliográficos
Autores principales:	Sarfraz, Nadia, Moscoso, Emilia, Oertel, Therese, Lee, Harrison J., Ranjit, Suman, Braselmann, Esther
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9935525/ https://www.ncbi.nlm.nih.gov/pubmed/36797241 http://dx.doi.org/10.1038/s41467-023-36531-y

Ejemplares similares

Establishing Riboglow-FLIM to visualize noncoding RNAs inside live zebrafish embryos
por: Sarfraz, Nadia, et al.
Publicado: (2023)

Use of Fluorescence Lifetime Imaging Microscopy (FLIM) as a Timer of Cell Cycle S Phase
por: Okkelman, Irina A., et al.
Publicado: (2016)

Streamlined histone-based fluorescence lifetime imaging microscopy (FLIM) for studying chromatin organisation
por: Sherrard, Alice, et al.
Publicado: (2018)

Fluorescence Lifetime Imaging Microscopy (FLIM) of Intracellular Transport by Means of Doubly Labelled siRNA Architectures
por: Doll, Larissa, et al.
Publicado: (2021)

Stability of retinol in liposomes as measured by fluorescence lifetime spectroscopy and FLIM
por: Sumrall, L., et al.
Publicado: (2023)

Signal peptide peptidase (SPP) dimer formation as assessed by fluorescence lifetime imaging microscopy (FLIM) in intact cells
por: Nyborg, Andrew C, et al.
Publicado: (2006)

Probing the Dynamics of Doxorubicin-DNA Intercalation during the Initial Activation of Apoptosis by Fluorescence Lifetime Imaging Microscopy (FLIM)
por: Chen, Nai-Tzu, et al.
Publicado: (2012)

Label-free assessment of pre-implantation embryo quality by the Fluorescence Lifetime Imaging Microscopy (FLIM)-phasor approach
por: Ma, Ning, et al.
Publicado: (2019)

Application of Rapid Fluorescence Lifetime Imaging Microscopy (RapidFLIM) to Examine Dynamics of Nanoparticle Uptake in Live Cells
por: Ahmed-Cox, Aria, et al.
Publicado: (2022)

Fluorescence Lifetime Imaging Microscopy (FLIM) reveals spatial-metabolic changes in 3D breast cancer spheroids
por: Karrobi, Kavon, et al.
Publicado: (2023)

Visualising varnish removal for conservation of paintings by fluorescence lifetime imaging (FLIM)
por: Wilda, Christine B., et al.
Publicado: (2023)

Accurate Rapid Lifetime Determination on Time-Gated FLIM Microscopy with Optical Sectioning
por: Silva, Susana F., et al.
Publicado: (2018)

Correction: Probing the Dynamics of Doxorubicin-DNA Intercalation during the Initial Activation of Apoptosis by Fluorescence Lifetime Imaging Microscopy (FLIM)
por: Chen, Nai-Tzu, et al.
Publicado: (2012)

Screening for protein-protein interactions using Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM)
por: Margineanu, Anca, et al.
Publicado: (2016)

Corrigendum: Screening for protein-protein interactions using Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM)
por: Margineanu, Anca, et al.
Publicado: (2016)

Adaptive optics for a time-resolved Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) in vivo
por: Coelho, Simao, et al.
Publicado: (2020)

A deeper understanding of intestinal organoid metabolism revealed by combining fluorescence lifetime imaging microscopy (FLIM) and extracellular flux analyses
por: Okkelman, Irina A., et al.
Publicado: (2019)

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM
por: Ishikawa-Ankerhold, Hellen C., et al.
Publicado: (2012)

A Rapid Method for Detecting Microplastics Based on Fluorescence Lifetime Imaging Technology (FLIM)
por: Zhou, Fang, et al.
Publicado: (2022)

Simultaneous Fluorescence and Phosphorescence Lifetime Imaging Microscopy in Living Cells
por: Jahn, Karolina, et al.
Publicado: (2015)

Machine Learning Methods for Fluorescence Lifetime Imaging (FLIM) Based Label-Free Detection of Microglia
por: Sagar, Md Abdul Kader, et al.
Publicado: (2020)

It's complicated: the interplay of Kif1c mRNA localization in cell protrusions, assembly of protein binding partners on the KIF1C protein, and cell migration
por: Sarfraz, Nadia, et al.
Publicado: (2023)

Quantifying nuclear wide chromatin compaction by phasor analysis of histone Förster resonance energy transfer (FRET) in frequency domain fluorescence lifetime imaging microscopy (FLIM) data
por: Liang, Zhen, et al.
Publicado: (2020)

Robust Bayesian Fluorescence Lifetime Estimation, Decay Model Selection and Instrument Response Determination for Low-Intensity FLIM Imaging
por: Rowley, Mark I., et al.
Publicado: (2016)

Practical and reliable FRET/FLIM pair of fluorescent proteins
por: Shcherbo, Dmitry, et al.
Publicado: (2009)

Correction: Robust Bayesian Fluorescence Lifetime Estimation, Decay Model Selection and Instrument Response Determination for Low-Intensity FLIM Imaging
por: Rowley, Mark I., et al.
Publicado: (2016)

Cleavable Linker Incorporation into a Synthetic Dye‐Nanobody‐Fluorescent Protein Assembly: FRET, FLIM and STED Microscopy
por: Aktalay, Ayse, et al.
Publicado: (2022)

Accepting from the best donor; analysis of long-lifetime donor fluorescent protein pairings to optimise dynamic FLIM-based FRET experiments
por: Martin, Kirsty J., et al.
Publicado: (2018)

FLIM-FRET analyzer: open source software for automation of lifetime-based FRET analysis
por: Kim, Jiho, et al.
Publicado: (2017)

In vivo melanin 3D quantification and z-epidermal distribution by multiphoton FLIM, phasor and Pseudo-FLIM analyses
por: Pena, Ana-Maria, et al.
Publicado: (2022)

Dynamic Cellular Cartography: Mapping the Local Determinants of Oligodendrocyte Transcription Factor 2 (OLIG2) Function in Live Cells Using Massively Parallel Fluorescence Correlation Spectroscopy Integrated with Fluorescence Lifetime Imaging Microscopy (mpFCS/FLIM)
por: Oasa, Sho, et al.
Publicado: (2021)

Metabolic fingerprinting of bacteria by fluorescence lifetime imaging microscopy
por: Bhattacharjee, Arunima, et al.
Publicado: (2017)

Light-field tomographic fluorescence lifetime imaging microscopy
por: Ma, Yayao, et al.
Publicado: (2023)

Separating NADH and NADPH fluorescence in live cells and tissues using FLIM
por: Blacker, Thomas S., et al.
Publicado: (2014)

The in vivo mechanics of the magnetotactic backbone as revealed by correlative FLIM-FRET and STED microscopy
por: Günther, Erika, et al.
Publicado: (2019)

A FLIM Microscopy Based on Acceptor-Detected Förster Resonance Energy Transfer
por: Delgadillo, Roberto F., et al.
Publicado: (2021)

Characterization of mitochondrial dysfunction due to laser damage by 2-photon FLIM microscopy
por: Alam, Shagufta Rehman, et al.
Publicado: (2022)

Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin
por: Seidenari, Stefania, et al.
Publicado: (2012)

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
por: Verboogen, Daniëlle R. J., et al.
Publicado: (2017)

Fluorescence Lifetime Imaging Microscopy of Porphyrins in Helicobacter pylori Biofilms
por: Battisti, Antonella, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_ovoiedct964pc8ohg6pr3ark05