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Precision-engineering of subunit vaccine particles for prevention of infectious diseases
Vaccines remain the best approach for the prevention of infectious diseases. Protein subunit vaccines are safe compared to live-attenuated whole cell vaccines but often show reduced immunogenicity. Subunit vaccines in particulate format show improved vaccine efficacy by inducing strong immune respon...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9935699/ https://www.ncbi.nlm.nih.gov/pubmed/36817419 http://dx.doi.org/10.3389/fimmu.2023.1131057 |
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author | Chen, Shuxiong Pounraj, Saranya Sivakumaran, Nivethika Kakkanat, Anjali Sam, Gayathri Kabir, Md. Tanvir Rehm, Bernd H. A. |
author_facet | Chen, Shuxiong Pounraj, Saranya Sivakumaran, Nivethika Kakkanat, Anjali Sam, Gayathri Kabir, Md. Tanvir Rehm, Bernd H. A. |
author_sort | Chen, Shuxiong |
collection | PubMed |
description | Vaccines remain the best approach for the prevention of infectious diseases. Protein subunit vaccines are safe compared to live-attenuated whole cell vaccines but often show reduced immunogenicity. Subunit vaccines in particulate format show improved vaccine efficacy by inducing strong immune responses leading to protective immunity against the respective pathogens. Antigens with proper conformation and function are often required to induce functional immune responses. Production of such antigens requiring post-translational modifications and/or composed of multiple complex domains in bacterial hosts remains challenging. Here, we discuss strategies to overcome these limitations toward the development of particulate vaccines eliciting desired humoral and cellular immune responses. We also describe innovative concepts of assembling particulate vaccine candidates with complex antigens bearing multiple post-translational modifications. The approaches include non-covalent attachments (e.g. biotin-avidin affinity) and covalent attachments (e.g. SpyCatcher-SpyTag) to attach post-translationally modified antigens to particles. |
format | Online Article Text |
id | pubmed-9935699 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99356992023-02-18 Precision-engineering of subunit vaccine particles for prevention of infectious diseases Chen, Shuxiong Pounraj, Saranya Sivakumaran, Nivethika Kakkanat, Anjali Sam, Gayathri Kabir, Md. Tanvir Rehm, Bernd H. A. Front Immunol Immunology Vaccines remain the best approach for the prevention of infectious diseases. Protein subunit vaccines are safe compared to live-attenuated whole cell vaccines but often show reduced immunogenicity. Subunit vaccines in particulate format show improved vaccine efficacy by inducing strong immune responses leading to protective immunity against the respective pathogens. Antigens with proper conformation and function are often required to induce functional immune responses. Production of such antigens requiring post-translational modifications and/or composed of multiple complex domains in bacterial hosts remains challenging. Here, we discuss strategies to overcome these limitations toward the development of particulate vaccines eliciting desired humoral and cellular immune responses. We also describe innovative concepts of assembling particulate vaccine candidates with complex antigens bearing multiple post-translational modifications. The approaches include non-covalent attachments (e.g. biotin-avidin affinity) and covalent attachments (e.g. SpyCatcher-SpyTag) to attach post-translationally modified antigens to particles. Frontiers Media S.A. 2023-02-03 /pmc/articles/PMC9935699/ /pubmed/36817419 http://dx.doi.org/10.3389/fimmu.2023.1131057 Text en Copyright © 2023 Chen, Pounraj, Sivakumaran, Kakkanat, Sam, Kabir and Rehm https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Chen, Shuxiong Pounraj, Saranya Sivakumaran, Nivethika Kakkanat, Anjali Sam, Gayathri Kabir, Md. Tanvir Rehm, Bernd H. A. Precision-engineering of subunit vaccine particles for prevention of infectious diseases |
title | Precision-engineering of subunit vaccine particles for prevention of infectious diseases |
title_full | Precision-engineering of subunit vaccine particles for prevention of infectious diseases |
title_fullStr | Precision-engineering of subunit vaccine particles for prevention of infectious diseases |
title_full_unstemmed | Precision-engineering of subunit vaccine particles for prevention of infectious diseases |
title_short | Precision-engineering of subunit vaccine particles for prevention of infectious diseases |
title_sort | precision-engineering of subunit vaccine particles for prevention of infectious diseases |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9935699/ https://www.ncbi.nlm.nih.gov/pubmed/36817419 http://dx.doi.org/10.3389/fimmu.2023.1131057 |
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