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Seasonal succession of microbial community co-occurrence patterns and community assembly mechanism in coal mining subsidence lakes

Coal mining subsidence lakes are classic hydrologic characteristics created by underground coal mining and represent severe anthropogenic disturbances and environmental challenges. However, the assembly mechanisms and diversity of microbial communities shaped by such environments are poorly understo...

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Autores principales: Fang, Wangkai, Fan, Tingyu, Xu, Liangji, Wang, Shun, Wang, Xingming, Lu, Akang, Chen, Yongchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9936157/
https://www.ncbi.nlm.nih.gov/pubmed/36819062
http://dx.doi.org/10.3389/fmicb.2023.1098236
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author Fang, Wangkai
Fan, Tingyu
Xu, Liangji
Wang, Shun
Wang, Xingming
Lu, Akang
Chen, Yongchun
author_facet Fang, Wangkai
Fan, Tingyu
Xu, Liangji
Wang, Shun
Wang, Xingming
Lu, Akang
Chen, Yongchun
author_sort Fang, Wangkai
collection PubMed
description Coal mining subsidence lakes are classic hydrologic characteristics created by underground coal mining and represent severe anthropogenic disturbances and environmental challenges. However, the assembly mechanisms and diversity of microbial communities shaped by such environments are poorly understood yet. In this study, we explored aquatic bacterial community diversity and ecological assembly processes in subsidence lakes during winter and summer using 16S rRNA gene sequencing. We observed that clear bacterial community structure was driven by seasonality more than by habitat, and the α-diversity and functional diversity of the bacterial community in summer were significantly higher than in winter (p < 0.001). Canonical correspondence analysis indicated that temperature and chlorophyll-a were the most crucial contributing factors influencing the community season variations in subsidence lakes. Specifically, temperature and chlorophyll-a explained 18.26 and 14.69% of the community season variation, respectively. The bacterial community variation was driven by deterministic processes in winter but dominated by stochastic processes in summer. Compared to winter, the network of bacterial communities in summer exhibited a higher average degree, modularity, and keystone taxa (hubs and connectors in a network), thereby forming a highly complex and stable community structure. These results illustrate the clear season heterogeneity of bacterial communities in subsidence lakes and provide new insights into revealing the effects of seasonal succession on microbial assembly processes in coal mining subsidence lake ecosystems.
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spelling pubmed-99361572023-02-18 Seasonal succession of microbial community co-occurrence patterns and community assembly mechanism in coal mining subsidence lakes Fang, Wangkai Fan, Tingyu Xu, Liangji Wang, Shun Wang, Xingming Lu, Akang Chen, Yongchun Front Microbiol Microbiology Coal mining subsidence lakes are classic hydrologic characteristics created by underground coal mining and represent severe anthropogenic disturbances and environmental challenges. However, the assembly mechanisms and diversity of microbial communities shaped by such environments are poorly understood yet. In this study, we explored aquatic bacterial community diversity and ecological assembly processes in subsidence lakes during winter and summer using 16S rRNA gene sequencing. We observed that clear bacterial community structure was driven by seasonality more than by habitat, and the α-diversity and functional diversity of the bacterial community in summer were significantly higher than in winter (p < 0.001). Canonical correspondence analysis indicated that temperature and chlorophyll-a were the most crucial contributing factors influencing the community season variations in subsidence lakes. Specifically, temperature and chlorophyll-a explained 18.26 and 14.69% of the community season variation, respectively. The bacterial community variation was driven by deterministic processes in winter but dominated by stochastic processes in summer. Compared to winter, the network of bacterial communities in summer exhibited a higher average degree, modularity, and keystone taxa (hubs and connectors in a network), thereby forming a highly complex and stable community structure. These results illustrate the clear season heterogeneity of bacterial communities in subsidence lakes and provide new insights into revealing the effects of seasonal succession on microbial assembly processes in coal mining subsidence lake ecosystems. Frontiers Media S.A. 2023-02-03 /pmc/articles/PMC9936157/ /pubmed/36819062 http://dx.doi.org/10.3389/fmicb.2023.1098236 Text en Copyright © 2023 Fang, Fan, Xu, Wang, Wang, Lu and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Fang, Wangkai
Fan, Tingyu
Xu, Liangji
Wang, Shun
Wang, Xingming
Lu, Akang
Chen, Yongchun
Seasonal succession of microbial community co-occurrence patterns and community assembly mechanism in coal mining subsidence lakes
title Seasonal succession of microbial community co-occurrence patterns and community assembly mechanism in coal mining subsidence lakes
title_full Seasonal succession of microbial community co-occurrence patterns and community assembly mechanism in coal mining subsidence lakes
title_fullStr Seasonal succession of microbial community co-occurrence patterns and community assembly mechanism in coal mining subsidence lakes
title_full_unstemmed Seasonal succession of microbial community co-occurrence patterns and community assembly mechanism in coal mining subsidence lakes
title_short Seasonal succession of microbial community co-occurrence patterns and community assembly mechanism in coal mining subsidence lakes
title_sort seasonal succession of microbial community co-occurrence patterns and community assembly mechanism in coal mining subsidence lakes
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9936157/
https://www.ncbi.nlm.nih.gov/pubmed/36819062
http://dx.doi.org/10.3389/fmicb.2023.1098236
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