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CRISPRi screen for enhancing heterologous α-amylase yield in Bacillus subtilis

Yield improvements in cell factories can potentially be obtained by fine-tuning the regulatory mechanisms for gene candidates. In pursuit of such candidates, we performed RNA-sequencing of two α-amylase producing Bacillus strains and predict hundreds of putative novel non-coding transcribed regions....

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Detalles Bibliográficos
Autores principales:	Geissler, Adrian Sven, Fehler, Annaleigh Ohrt, Poulsen, Line Dahl, González-Tortuero, Enrique, Kallehauge, Thomas Beuchert, Alkan, Ferhat, Anthon, Christian, Seemann, Stefan Ernst, Rasmussen, Michael Dolberg, Breüner, Anne, Hjort, Carsten, Vinther, Jeppe, Gorodkin, Jan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2022
Materias:	Original Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9936203/ https://www.ncbi.nlm.nih.gov/pubmed/36564025 http://dx.doi.org/10.1093/jimb/kuac028

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