Application of transposon insertion site sequencing method in the exploration of gene function in microalgae

Microalgae are a large group of organisms that can produce various useful substances through photosynthesis. Microalgae need to be genetically modified at the molecular level to become “Chassis Cells” for food, medicine, energy, and environmental protection and, consequently, obtain benefits from mi...

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Autores principales: Hu, Xiaobing, Fan, Yulong, Mao, Chengfeng, Chen, Hui, Wang, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9936982/
https://www.ncbi.nlm.nih.gov/pubmed/36819037
http://dx.doi.org/10.3389/fmicb.2023.1111794
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author Hu, Xiaobing
Fan, Yulong
Mao, Chengfeng
Chen, Hui
Wang, Qiang
author_facet Hu, Xiaobing
Fan, Yulong
Mao, Chengfeng
Chen, Hui
Wang, Qiang
author_sort Hu, Xiaobing
collection PubMed
description Microalgae are a large group of organisms that can produce various useful substances through photosynthesis. Microalgae need to be genetically modified at the molecular level to become “Chassis Cells” for food, medicine, energy, and environmental protection and, consequently, obtain benefits from microalgae resources. Insertional mutagenesis of microalgae using transposons is a practical possibility for understanding the function of microalgae genes. Theoretical and technical support is provided in this manuscript for applying transposons to microalgae gene function by summarizing the sequencing method of transposon insertion sites.
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spelling pubmed-99369822023-02-18 Application of transposon insertion site sequencing method in the exploration of gene function in microalgae Hu, Xiaobing Fan, Yulong Mao, Chengfeng Chen, Hui Wang, Qiang Front Microbiol Microbiology Microalgae are a large group of organisms that can produce various useful substances through photosynthesis. Microalgae need to be genetically modified at the molecular level to become “Chassis Cells” for food, medicine, energy, and environmental protection and, consequently, obtain benefits from microalgae resources. Insertional mutagenesis of microalgae using transposons is a practical possibility for understanding the function of microalgae genes. Theoretical and technical support is provided in this manuscript for applying transposons to microalgae gene function by summarizing the sequencing method of transposon insertion sites. Frontiers Media S.A. 2023-02-03 /pmc/articles/PMC9936982/ /pubmed/36819037 http://dx.doi.org/10.3389/fmicb.2023.1111794 Text en Copyright © 2023 Hu, Fan, Mao, Chen and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hu, Xiaobing
Fan, Yulong
Mao, Chengfeng
Chen, Hui
Wang, Qiang
Application of transposon insertion site sequencing method in the exploration of gene function in microalgae
title Application of transposon insertion site sequencing method in the exploration of gene function in microalgae
title_full Application of transposon insertion site sequencing method in the exploration of gene function in microalgae
title_fullStr Application of transposon insertion site sequencing method in the exploration of gene function in microalgae
title_full_unstemmed Application of transposon insertion site sequencing method in the exploration of gene function in microalgae
title_short Application of transposon insertion site sequencing method in the exploration of gene function in microalgae
title_sort application of transposon insertion site sequencing method in the exploration of gene function in microalgae
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9936982/
https://www.ncbi.nlm.nih.gov/pubmed/36819037
http://dx.doi.org/10.3389/fmicb.2023.1111794
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