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Study of Camellia sinensis diploid and triploid leaf development mechanism based on transcriptome and leaf characteristics

Polyploidization results in significant changes in the morphology and physiology of plants, with increased growth rate and genetic gains as the number of chromosomes increases. In this study, the leaf functional traits, photosynthetic characteristics, leaf cell structure and transcriptome of Camelli...

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Autores principales: Yao, Xinzhuan, Qi, Yong, Chen, Hufang, Zhang, Baohui, Chen, Zhengwu, Lu, Litang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9937487/
https://www.ncbi.nlm.nih.gov/pubmed/36800382
http://dx.doi.org/10.1371/journal.pone.0275652
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author Yao, Xinzhuan
Qi, Yong
Chen, Hufang
Zhang, Baohui
Chen, Zhengwu
Lu, Litang
author_facet Yao, Xinzhuan
Qi, Yong
Chen, Hufang
Zhang, Baohui
Chen, Zhengwu
Lu, Litang
author_sort Yao, Xinzhuan
collection PubMed
description Polyploidization results in significant changes in the morphology and physiology of plants, with increased growth rate and genetic gains as the number of chromosomes increases. In this study, the leaf functional traits, photosynthetic characteristics, leaf cell structure and transcriptome of Camellia sinensis were analyzed. The results showed that triploid tea had a significant growth advantage over diploid tea, the leaf area was 59.81% larger, and the photosynthetic capacity was greater. The morphological structure of triploid leaves was significantly different, the xylem of the veins was more developed, the cell gap between the palisade tissue and the sponge tissue was larger and the stomata of the triploid leaves were also larger. Transcriptome sequencing analysis revealed that in triploid tea, the changes in leaf morphology and physiological characteristics were affected by the expression of certain key regulatory genes. We identified a large number of genes that may play important roles in leaf development, especially genes involved in photosynthesis, cell division, hormone synthesis and stomata development. This research will enhance our understanding of the molecular mechanism underlying tea and stomata development and provide a basis for molecular breeding of high-quality and high-yield tea varieties.
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spelling pubmed-99374872023-02-18 Study of Camellia sinensis diploid and triploid leaf development mechanism based on transcriptome and leaf characteristics Yao, Xinzhuan Qi, Yong Chen, Hufang Zhang, Baohui Chen, Zhengwu Lu, Litang PLoS One Research Article Polyploidization results in significant changes in the morphology and physiology of plants, with increased growth rate and genetic gains as the number of chromosomes increases. In this study, the leaf functional traits, photosynthetic characteristics, leaf cell structure and transcriptome of Camellia sinensis were analyzed. The results showed that triploid tea had a significant growth advantage over diploid tea, the leaf area was 59.81% larger, and the photosynthetic capacity was greater. The morphological structure of triploid leaves was significantly different, the xylem of the veins was more developed, the cell gap between the palisade tissue and the sponge tissue was larger and the stomata of the triploid leaves were also larger. Transcriptome sequencing analysis revealed that in triploid tea, the changes in leaf morphology and physiological characteristics were affected by the expression of certain key regulatory genes. We identified a large number of genes that may play important roles in leaf development, especially genes involved in photosynthesis, cell division, hormone synthesis and stomata development. This research will enhance our understanding of the molecular mechanism underlying tea and stomata development and provide a basis for molecular breeding of high-quality and high-yield tea varieties. Public Library of Science 2023-02-17 /pmc/articles/PMC9937487/ /pubmed/36800382 http://dx.doi.org/10.1371/journal.pone.0275652 Text en © 2023 Yao et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yao, Xinzhuan
Qi, Yong
Chen, Hufang
Zhang, Baohui
Chen, Zhengwu
Lu, Litang
Study of Camellia sinensis diploid and triploid leaf development mechanism based on transcriptome and leaf characteristics
title Study of Camellia sinensis diploid and triploid leaf development mechanism based on transcriptome and leaf characteristics
title_full Study of Camellia sinensis diploid and triploid leaf development mechanism based on transcriptome and leaf characteristics
title_fullStr Study of Camellia sinensis diploid and triploid leaf development mechanism based on transcriptome and leaf characteristics
title_full_unstemmed Study of Camellia sinensis diploid and triploid leaf development mechanism based on transcriptome and leaf characteristics
title_short Study of Camellia sinensis diploid and triploid leaf development mechanism based on transcriptome and leaf characteristics
title_sort study of camellia sinensis diploid and triploid leaf development mechanism based on transcriptome and leaf characteristics
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9937487/
https://www.ncbi.nlm.nih.gov/pubmed/36800382
http://dx.doi.org/10.1371/journal.pone.0275652
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