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A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins
The western corn rootworm (WCR) Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) remains one of the economically most important pests of maize (Zea mays) due to its adaptive capabilities to pest management options. This includes the ability to develop resistance to some of the commercial p...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9937505/ https://www.ncbi.nlm.nih.gov/pubmed/36800363 http://dx.doi.org/10.1371/journal.pone.0267220 |
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author | Jabeur, Rania Guyon, Virginie Toth, Szabolcs Pereira, Adriano E. Huynh, Man P. Selmani, Zakia Boland, Erin Bosio, Mickael Beuf, Laurent Clark, Pete Vallenet, David Achouak, Wafa Audiffrin, Carine Torney, François Paul, Wyatt Heulin, Thierry Hibbard, Bruce E. Toepfer, Stefan Sallaud, Christophe |
author_facet | Jabeur, Rania Guyon, Virginie Toth, Szabolcs Pereira, Adriano E. Huynh, Man P. Selmani, Zakia Boland, Erin Bosio, Mickael Beuf, Laurent Clark, Pete Vallenet, David Achouak, Wafa Audiffrin, Carine Torney, François Paul, Wyatt Heulin, Thierry Hibbard, Bruce E. Toepfer, Stefan Sallaud, Christophe |
author_sort | Jabeur, Rania |
collection | PubMed |
description | The western corn rootworm (WCR) Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) remains one of the economically most important pests of maize (Zea mays) due to its adaptive capabilities to pest management options. This includes the ability to develop resistance to some of the commercial pesticidal proteins originating from different strains of Bacillus thuringiensis. Although urgently needed, the discovery of new, environmentally safe agents with new modes of action is a challenge. In this study we report the discovery of a new family of binary pesticidal proteins isolated from several Chryseobacterium species. These novel binary proteins, referred to as GDI0005A and GDI0006A, produced as recombinant proteins, prevent growth and increase mortality of WCR larvae, as does the bacteria. These effects were found both in susceptible and resistant WCR colonies to Cry3Bb1 and Cry34Ab1/Cry35Ab1 (reassigned Gpp34Ab1/Tpp35Ab1). This suggests GDI0005A and GDI0006A may not share the same binding sites as those commercially deployed proteins and thereby possess a new mode of action. This paves the way towards the development of novel biological or biotechnological management solutions urgently needed against rootworms. |
format | Online Article Text |
id | pubmed-9937505 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-99375052023-02-18 A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins Jabeur, Rania Guyon, Virginie Toth, Szabolcs Pereira, Adriano E. Huynh, Man P. Selmani, Zakia Boland, Erin Bosio, Mickael Beuf, Laurent Clark, Pete Vallenet, David Achouak, Wafa Audiffrin, Carine Torney, François Paul, Wyatt Heulin, Thierry Hibbard, Bruce E. Toepfer, Stefan Sallaud, Christophe PLoS One Research Article The western corn rootworm (WCR) Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) remains one of the economically most important pests of maize (Zea mays) due to its adaptive capabilities to pest management options. This includes the ability to develop resistance to some of the commercial pesticidal proteins originating from different strains of Bacillus thuringiensis. Although urgently needed, the discovery of new, environmentally safe agents with new modes of action is a challenge. In this study we report the discovery of a new family of binary pesticidal proteins isolated from several Chryseobacterium species. These novel binary proteins, referred to as GDI0005A and GDI0006A, produced as recombinant proteins, prevent growth and increase mortality of WCR larvae, as does the bacteria. These effects were found both in susceptible and resistant WCR colonies to Cry3Bb1 and Cry34Ab1/Cry35Ab1 (reassigned Gpp34Ab1/Tpp35Ab1). This suggests GDI0005A and GDI0006A may not share the same binding sites as those commercially deployed proteins and thereby possess a new mode of action. This paves the way towards the development of novel biological or biotechnological management solutions urgently needed against rootworms. Public Library of Science 2023-02-17 /pmc/articles/PMC9937505/ /pubmed/36800363 http://dx.doi.org/10.1371/journal.pone.0267220 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Jabeur, Rania Guyon, Virginie Toth, Szabolcs Pereira, Adriano E. Huynh, Man P. Selmani, Zakia Boland, Erin Bosio, Mickael Beuf, Laurent Clark, Pete Vallenet, David Achouak, Wafa Audiffrin, Carine Torney, François Paul, Wyatt Heulin, Thierry Hibbard, Bruce E. Toepfer, Stefan Sallaud, Christophe A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins |
title | A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins |
title_full | A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins |
title_fullStr | A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins |
title_full_unstemmed | A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins |
title_short | A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins |
title_sort | novel binary pesticidal protein from chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9937505/ https://www.ncbi.nlm.nih.gov/pubmed/36800363 http://dx.doi.org/10.1371/journal.pone.0267220 |
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