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A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins

The western corn rootworm (WCR) Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) remains one of the economically most important pests of maize (Zea mays) due to its adaptive capabilities to pest management options. This includes the ability to develop resistance to some of the commercial p...

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Autores principales: Jabeur, Rania, Guyon, Virginie, Toth, Szabolcs, Pereira, Adriano E., Huynh, Man P., Selmani, Zakia, Boland, Erin, Bosio, Mickael, Beuf, Laurent, Clark, Pete, Vallenet, David, Achouak, Wafa, Audiffrin, Carine, Torney, François, Paul, Wyatt, Heulin, Thierry, Hibbard, Bruce E., Toepfer, Stefan, Sallaud, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9937505/
https://www.ncbi.nlm.nih.gov/pubmed/36800363
http://dx.doi.org/10.1371/journal.pone.0267220
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author Jabeur, Rania
Guyon, Virginie
Toth, Szabolcs
Pereira, Adriano E.
Huynh, Man P.
Selmani, Zakia
Boland, Erin
Bosio, Mickael
Beuf, Laurent
Clark, Pete
Vallenet, David
Achouak, Wafa
Audiffrin, Carine
Torney, François
Paul, Wyatt
Heulin, Thierry
Hibbard, Bruce E.
Toepfer, Stefan
Sallaud, Christophe
author_facet Jabeur, Rania
Guyon, Virginie
Toth, Szabolcs
Pereira, Adriano E.
Huynh, Man P.
Selmani, Zakia
Boland, Erin
Bosio, Mickael
Beuf, Laurent
Clark, Pete
Vallenet, David
Achouak, Wafa
Audiffrin, Carine
Torney, François
Paul, Wyatt
Heulin, Thierry
Hibbard, Bruce E.
Toepfer, Stefan
Sallaud, Christophe
author_sort Jabeur, Rania
collection PubMed
description The western corn rootworm (WCR) Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) remains one of the economically most important pests of maize (Zea mays) due to its adaptive capabilities to pest management options. This includes the ability to develop resistance to some of the commercial pesticidal proteins originating from different strains of Bacillus thuringiensis. Although urgently needed, the discovery of new, environmentally safe agents with new modes of action is a challenge. In this study we report the discovery of a new family of binary pesticidal proteins isolated from several Chryseobacterium species. These novel binary proteins, referred to as GDI0005A and GDI0006A, produced as recombinant proteins, prevent growth and increase mortality of WCR larvae, as does the bacteria. These effects were found both in susceptible and resistant WCR colonies to Cry3Bb1 and Cry34Ab1/Cry35Ab1 (reassigned Gpp34Ab1/Tpp35Ab1). This suggests GDI0005A and GDI0006A may not share the same binding sites as those commercially deployed proteins and thereby possess a new mode of action. This paves the way towards the development of novel biological or biotechnological management solutions urgently needed against rootworms.
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spelling pubmed-99375052023-02-18 A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins Jabeur, Rania Guyon, Virginie Toth, Szabolcs Pereira, Adriano E. Huynh, Man P. Selmani, Zakia Boland, Erin Bosio, Mickael Beuf, Laurent Clark, Pete Vallenet, David Achouak, Wafa Audiffrin, Carine Torney, François Paul, Wyatt Heulin, Thierry Hibbard, Bruce E. Toepfer, Stefan Sallaud, Christophe PLoS One Research Article The western corn rootworm (WCR) Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) remains one of the economically most important pests of maize (Zea mays) due to its adaptive capabilities to pest management options. This includes the ability to develop resistance to some of the commercial pesticidal proteins originating from different strains of Bacillus thuringiensis. Although urgently needed, the discovery of new, environmentally safe agents with new modes of action is a challenge. In this study we report the discovery of a new family of binary pesticidal proteins isolated from several Chryseobacterium species. These novel binary proteins, referred to as GDI0005A and GDI0006A, produced as recombinant proteins, prevent growth and increase mortality of WCR larvae, as does the bacteria. These effects were found both in susceptible and resistant WCR colonies to Cry3Bb1 and Cry34Ab1/Cry35Ab1 (reassigned Gpp34Ab1/Tpp35Ab1). This suggests GDI0005A and GDI0006A may not share the same binding sites as those commercially deployed proteins and thereby possess a new mode of action. This paves the way towards the development of novel biological or biotechnological management solutions urgently needed against rootworms. Public Library of Science 2023-02-17 /pmc/articles/PMC9937505/ /pubmed/36800363 http://dx.doi.org/10.1371/journal.pone.0267220 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Jabeur, Rania
Guyon, Virginie
Toth, Szabolcs
Pereira, Adriano E.
Huynh, Man P.
Selmani, Zakia
Boland, Erin
Bosio, Mickael
Beuf, Laurent
Clark, Pete
Vallenet, David
Achouak, Wafa
Audiffrin, Carine
Torney, François
Paul, Wyatt
Heulin, Thierry
Hibbard, Bruce E.
Toepfer, Stefan
Sallaud, Christophe
A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins
title A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins
title_full A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins
title_fullStr A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins
title_full_unstemmed A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins
title_short A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins
title_sort novel binary pesticidal protein from chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9937505/
https://www.ncbi.nlm.nih.gov/pubmed/36800363
http://dx.doi.org/10.1371/journal.pone.0267220
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